Phenolic and Flavonoid Responses of Bauji Shallots to Ammonium Sulfate under Floating Cultivation

Syafira Zulfa Hidayanti, Susilawati Susilawati, Suwandi Suwandi

Abstract

Shallot is a high value horticultural crop valued for its flavor, aroma, and medicinal properties, and is rich in phenolics and flavonoids that contribute to antioxidant activity. Limited dryland and fluctuating demand in South Sumatra highlight the need for alternative systems such as floating cultivation. This study aimed to evaluate the effects of different ammonium sulfate (ZA) doses on the bulb yield, total phenolic, and flavonoid contents of Bauji shallots grown under floating cultivation. The experiment used a randomized complete block design with three replications and four ZA fertilizer treatments (0, 60, 180, and 360 kg ha⁻¹). Observed variables included total phenolic and flavonoid contents measured by spectrophotometry and dry bulb weight. The results indicated that the ZA application significantly increased dry bulb weight and total phenolic content but did not affect flavonoids. The highest bulb yield and flavonoid content were obtained with 60 kg ha⁻¹, whereas phenolics peaked under the control (0 kg ha⁻¹), suggesting a trade-off between yield and secondary metabolite accumulation. Correlation analysis revealed a positive association between bulb weight and flavonoid content, whereas phenolics were negatively related to fertilizer input. As the first report on Bauji shallots under floating cultivation, these findings indicate that moderate ZA fertilization (60 kg ha⁻¹) optimizes bulb yield while maintaining bioactive compound levels. However, further multi-season studies are needed for broader recommendations.

Keywords

fertilization; flavonoid content; medicinal plant; phenolic compounds; plant extracts

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References

Al-Khayri, J. M., Sahana, G. R., Nagella, P., Joseph, B. V., Alessa, F. M., & Al-Mssallem, M. Q. (2022). Flavonoids as potential anti-inflammatory molecules: A review. Molecules, 27(9), 2901. https://doi.org/10.3390/molecules27092901

Aminah, Tomayahu, N., & Abidin, Z. (2017). Penetapan kadar flavonoid total ekstrak etanol kulit buah alpukat (Persea americana Mill.) dengan metode spektofotometri UV-Vis. Jurnal Fitofarmaka Indonesia, 4(2), 226–230. https://doi.org/10.33096/jffi.v4i2.265

Armanto, M. E., Wildayana, E., & Syakina, B. (2018). Dynamics, degradation and future challenges of wetlands in South Sumatra Province, Indonesia. E3S Web of Conferences, 68(1), 04001. https://doi.org/10.1051/e3sconf/20186804001

Buchory, G. N., Anwar, S., & Kristanto, B. A. (2020). Pertumbuhan, produksi simplisia, dan kandungan fenolik total selasih (Ocimum basilicum L.) pada berbagai taraf cekaman kekeringan dan waktu panen. Jurnal Agrotek, 5(2), 37–48. https://doi.org/10.33096/agrotek.v5i2.166

Chen, L. H., Xu, M., Cheng, Z., & Yang, L. T. (2024). Effects of nitrogen deficiency on the photosynthesis, chlorophyll a fluorescence, antioxidant system, and sulfur compounds in Oryza sativa. International Journal of Molecular Sciences, 25(19), 10409. https://doi.org/10.3390/ijms251910409

Davies, K. M., Jibran, R., Zhou, Y., Albert, N. W., Brummell, D. A., Jordan, B. R., ... & Schwinn, K. E. (2020). The evolution of flavonoid biosynthesis: A bryophyte perspective. Frontiers in Plant Science, 11(7), 508087. https://doi.org/10.3389/fpls.2020.00007

Hadiawati, L., Fitrotin, U., Suriadi, A., & Nazam, M. (2023). Application of silicon foliar spray to increase growth and yield of shallot (Allium ascalonicum L.) under sprinkler and furrow irrigation system. IOP Conference Series: Earth and Environmental Science, 1253(1), 012056. https://doi.org/10.1088/1755-1315/1253/1/012056

Hasanah, Y., Mawarni, L., Hanum, H., Sipayung, R., & Ramadhan, M. T. (2021). The role of sulfur and paclobutrazol on the growth of shallots (Allium ascalonicum (L.) Sanren F-1 varieties from true shallot seed. IOP Conference Series: Earth and Environmental Science, 782(4), 042039. https://doi.org/10.1088/1755-1315/782/4/042039

Hasbi, H., Lakitan, B., & Herlinda, S. (2017). Persepsi petani terhadap budidaya cabai sistem pertanian terapung di Desa Pelabuhan Dalam, Ogan Ilir. Jurnal Lahan Suboptimal, 6(2), 126–133. https://doi.org/10.33230/JLSO.6.2.2017.297

Hasrianda, E. F., & Setiarto, R. H. B. (2022). Potensi rekayasa genetik bawang putih terhadap kandungan senyawa komponen bioaktif allicin dan kajian sifat fungsionalnya. Jurnal Pangan, 31(2), 167–190. https://doi.org/10.33964/jp.v31i2.586

He, J., Wang, Y., Li, H., Ma, J., Yue, X., Liang, X., ... & Liu, R. (2025). Controlled release fertilizer improving paddy yield and nitrogen use efficiency by reducing soil residual nitrogen and leaching losses in the yellow river irrigation area. Plants, 14(3), 408. https://doi.org/10.3390/plants14030408

Idly, N. S., Susilawati, S., Suwandi, S., & Oksilia, O. (2024). Aplikasi sulfur terhadap karakter agronomi, morfologi dan aktivitas antioksidan bawang merah (Allium ascalonicum L.) dibudidaya terapung. Jurnal Penelitian Pertanian Terapan, 24(4), 619–629. https://doi.org/10.25181/jppt.v24i4.3449

Jin, Q., Liu, T., Qiao, Y., Liu, D., Yang, L., Mao, H., ... & Zhan, Y. (2023). Oxidative stress and inflammation in diabetic nephropathy: Role of polyphenols. Frontiers in Immunology, 14, 1185317. https://doi.org/10.3389/fimmu.2023.1185317

Kopustinskiene, D. M., Jakstas, V., Savickas, A., & Bernatoniene, J. (2020). Flavonoids as anticancer agents. Nutrients, 12(2), 457. https://doi.org/10.3390/nu12020457Lestari, Y., & Maftu’Ah, E. (2021). Amelioration and variety selection to increase shallot yield in peatlands. IOP Conference Series: Earth and Environmental Science, 648(1), 012168. https://doi.org/10.1088/1755-1315/648/1/012168

Ma, Y., Zhang, S., Feng, D., Duan, N., Rong, L., Wu, Z., & Shen, Y. (2023). Effect of different doses of nitrogen fertilization on bioactive compounds and antioxidant activity of brown rice. Frontiers in Nutrition, 10, 1071874. https://doi.org/10.3389/fnut.2023.1071874

Marlin, M., Simarmata, M., Salamah, U., & Nurcholis, W. (2022). Effect of nitrogen and potassium application on growth, total phenolic, flavonoid contents, and antioxidant activity of Eleutherine palmifolia. AIMS Agriculture and Food, 7(3), 580–593. https://doi.org/10.3934/agrfood.2022036

Maulana, F., Batubara, I., & Nurcholis, W. (2024). Productivity of phenolic, flavonoid, and antioxidant in Justicia gendarussa Burm. f. by different shade and dose of nitrogen fertilizer. Yuzuncu Yil University Journal of Agricultural Sciences, 34(4), 596–607. https://doi.org/10.29133/yyutbd.1300943

Muthia, R., Bin Jamaludin, W., & Damayanti, L. (2023). Karakterisasi dan penetapan kadar fenol total ekstrak etanol umbi bawang dayak (Eleutherine bulbosa urb.) berdasarkan variasi waktu tumbuh tanaman. Jurnal Ilmiah Farmasi (Scientific Journal of Pharmacy) Special Edition, 83–93. Retrieved from https://journal.uii.ac.id/JIF/article/view/28991

Narvekar, A. S., & Tharayil, N. (2021). Nitrogen fertilization influences the quantity, composition, and tissue association of foliar phenolics in strawberries. Frontiers in Plant Science, 12, 613839. https://doi.org/10.3389/fpls.2021.613839

Powlson, D. S., & Dawson, C. J. (2022). Use of ammonium sulphate as a sulphur fertilizer: Implications for ammonia volatilization. Soil Use and Management, 38(1), 622–634. https://doi.org/10.1111/sum.12733

Pyka, L. M., Al-Maruf, A. A., Braun, B., Shamsuzzoha, M., & Jenkins, J. C. (2020). Floating gardening in coastal Bangladesh: Evidence of sustainable farming for food security under climate change. Journal of Agriculture, Food and Environment, 01(04), 161–168. https://doi.org/10.47440/jafe.2020.1424

Ramadhani, N., Samudra, A. G., & Pratiwi, L. W. I. (2020). Analisis penetapan kadar flavonoid sari jeruk kalamansi (Citrofortunella microcarpa) dengan metode spektrofotometri UV-VIS. Jurnal Mandala Pharmacon Indonesia, 6(01), 53–58. https://doi.org/10.35311/jmpi.v6i01.57

Rosyada, A. G., Prihastuti, C. C., Sari, D. N. I., Setiawati, S., Ichsyani, M., Laksitasari, A., ... & Kurniawan, A. A. (2023). Aktivitas antibiofilm ekstrak etanol kulit bawang merah (Allium cepa L.) dalam menghambat pembentukan biofilm Staphylococcus aureus ATCC 25923. Jurnal Kedokteran Gigi Universitas Padjadjaran, 35(1), 34–40. https://doi.org/10.24198/jkg.v35i1.42451

Rosyid, A. H. A., Viana, C. D. N., & Saputro, W. A. (2021). Penerapan model box jenkins (ARIMA) dalam peramalan harga konsumen bawang merah di Provinsi Jawa Tengah. Jurnal Agri Wiralodra, 13(1), 29–37. https://doi.org/10.31943/agriwiralodra.v13i1.19

Rudrapal, M., Khairnar, S. J., Khan, J., Dukhyil, A. B., Ansari, M. A., Alomary, M. N., ... & Devi, R. (2022). Dietary polyphenols and their role in oxidative stress-induced human diseases: Insights into protective effects, antioxidant potentials and mechanism(s) of action. Frontiers in Pharmacology, 13, 806470. https://doi.org/10.3389/fphar.2022.806470

Sarjani, A. S., Palupi, E. R., Suhartanto, M. R., & Purwanto, Y. A. (2018). Pengaruh suhu ruang simpan dan perlakuan pasca penyimpanan terhadap mutu dan produktivitas umbi benih bawang merah (Allium cepa L. group Aggregatum). Jurnal Hortikultura Indonesia, 9(2), 111–121. https://doi.org/10.29244/jhi.9.2.111-121

Shafira, A. E., Aziz, S. A., Farid, M., Ridwan, T., & Batubara, I. (2024). Effect of light intensities and nitrogen fertilizer dosages on growth, phenolics, and flavonoid production of Adenostemma lavenia. Journal of Agricultural Engineering, 13(1), 113–122. https://doi.org/10.23960/jtep-l.v13i1.114-123

Shahrajabian, M. H., Sun, W., & Cheng, Q. (2020). Chinese onion, and shallot, originated in Asia, medicinal plants for healthy daily recipes. Notulae Scientia Biologicae, 12(2), 197–207. https://doi.org/10.15835/nsb12210725

Sharma, A., Shahzad, B., Rehman, A., Bhardwaj, R., Landi, M., & Zheng, B. (2019). Response of phenylpropanoid pathway and the role of polyphenols in plants under abiotic stress. Molecules, 24(13), 2452. https://doi.org/10.3390/molecules24132452

Shi, T., Su, Y., Lan, Y., Duan, C., & Yu, K. (2024). The molecular basis of flavonoid biosynthesis response to water, light, and temperature in grape berries. Frontiers in Plant Science, 15(1), 1441893. https://doi.org/10.3389/fpls.2024.1441893

Siaga, E., Lakitan, B., & Bernas, S. M. (2019). Floating seedbed for preparing rice seedlings under unpredictable flooding occurrence at tropical riparian Wetland. Bulgarian Journal of Agricultural Science, 25(2), 326–336. Retrieved from https://www.agrojournal.org/25/02-16.pdf

Sofian, A., Rahim, S. E., Rosmiah, R., Aminah, I. S., Astuti, D. T., Amir, N., ... & Lusia, M. (2023). Application of floating rice science and technology on the Lebak Swamp Land in Agrotourism Tekno 44 Gelebak Dalam Village. Altifani Journal: International Journal of Community Engagement, 4(1), 46–51. https://doi.org/10.32502/altifani.v4i1.6886

Solagracya, J. H. P., Hanum, H., & Sarifuddin. (2025). Optimizing shallot plant growth and plant nutrient through site-specific fertilizer dose evaluation. Indonesian Journal of Agricultural Research, 8(1), 32–39. https://doi.org/10.32734/injar.v8i1.17133

Statistics of Indonesia. (2024). Weekly average consumption of several food items commodity per capita, 2007-2024. Retrieved from https://www.bps.go.id/id/statistics-table/1/OTUwIzE%3D/rata-rata-konsumsi-per-kapita-seminggu-beberapa-macam-bahan-makanan-penting--2007-2022.html?

Statistics of South Sumatra Province. (2024a). Population by province (thousand people), 2024. Retrieved from https://sumsel.bps.go.id/id/statistics-table/2/NTczIzI%3D/jumlah-penduduk-menurut-provinsi.html?

Statistics of South Sumatra Province. (2024b). Horticultural crop statistics of South Sumatra Province 2023. Retrieved from https://sumsel.bps.go.id/id/publication/2024/07/01/8207449e54f1d4c1ee04bddd/statistik-tanaman-hortikultura-provinsi-sumatera-selatan-2023.html

Suminartika, E., Deliana, Y., Hapsari, H., & Fatimah, S. (2022). The effect of input factor and optimization of input factor of shallot farm. IOP Conference Series: Earth and Environmental Science, 1107(1), 012110. https://doi.org/10.1088/1755-1315/1107/1/012110

Susilawati, Irmawati, Sukarmi, S., Ammar, M., Kurnianingsih, A., Yusnita, & Yayandra. (2021). Growth and yield of shallot under several levels of soil water. Russian Journal of Agricultural and Socio-Economic Sciences, 114(6), 199–206. https://doi.org/10.18551/rjoas.2021-06.23

Susilawati, Irmawati, Sukarmi, S., & Ammar, M. (2022). The application of chicken manure and NPK fertilizer on growth and yield of shallot plant in tidal land of Banyuasin Regency. Jurnal Lahan Suboptimal : Journal of Suboptimal Lands, 11(2), 197–205. https://doi.org/10.36706/jlso.11.2.2022.582

Tome, V. D., Rai, I. N., Dwiyani, R., & Wijana, G. (2022). Effect of quality seed and improvement of cultivation technology to increase the yield of shallots (Allium cepa L. Var. Aggregatum). GSC Biological and Pharmaceutical Sciences, 21(1), 238–246. https://doi.org/10.30574/gscbps.2022.21.1.0306

Wawan, & Fikrawati. (2021). Hasil tanaman selada (Lactuca sativa L.) pada komposisi medium berbeda yang dipupuk dengan urea dalam sistem budidaya terapung lahan rawa gambut. Jurnal Agroekotek, 13(2), 153–165. Retrieved from https://jurnal.untirta.ac.id/index.php/jav/article/download/13155/8121

Wildayana, E., & Armanto, M. E. (2018). Lebak swamp typology and rice production potency in Jakabaring South Sumatra. Agriekonomika, 7(1), 30–36. https://doi.org/10.21107/agriekonomika.v7i1.2513

Wu, H., Naren, G., Han, C., Elsheery, N. I., & Zhang, L. (2024). Exploring the flavonoid biosynthesis pathway of two ecotypes of Leymus chinensis using transcriptomic and metabolomic analysis. Agronomy, 14(8), 1839. https://doi.org/10.3390/agronomy14081839

Xie, N., Guo, Q., Li, H., Yuan, G., Gui, Q., Xiao, Y., ... & Yang, L. (2024). Integrated transcriptomic and WGCNA analyses reveal candidate genes regulating mainly flavonoid biosynthesis in Litsea coreana var. sinensis. BMC Plant Biology, 24(1), 231. https://doi.org/10.1186/s12870-024-04949-1

Yadav, V., Wang, Z., Wei, C., Amo, A., Ahmed, B., Yang, X., & Zhang, X. (2020). Phenylpropanoid pathway engineering: An emerging approach towards plant defense. Pathogens, 9(4), 312. https://doi.org/10.3390/pathogens9040312

Ye, Z., Qin, N., Fu, S., Zhang, H., Zhu, C., Dai, S., ... & Li, J. (2025). Targeted metabolomic and transcriptomic analyses provide insights into flavonoid biosynthesis in the grain of Foxtail millet. BMC Genomics, 26(1), 610. https://doi.org/10.1186/s12864-025-11780-x

Yuniarti, E., Noviyanti, E., Radiastuti, N., Kosasih, J., Aminah, S., Suryadi, Y., & Susilowati, D. N. (2023). Shallot growth and production responses to application of microorganisms based-biostimulant and NPK fertilizer combinations on acid soil. IOP Conference Series: Earth and Environmental Science, 1253(1), 012040. https://doi.org/10.1088/1755-1315/1253/1/012040

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