Optimization of Antibacterial Edible Film Formulation Based on Chitosan, Velvet Bean Ethanol Extract, and Cinnamon Essential Oil
Abstract
The existing research on edible films as packaging materials has maintained that they are better in constituent materials, composition, and functionality. Due to their good bioactivity, edible films are utilized for packaging, which is considered bioactive. The recent study focused on using chitosan-velvet bean (Mucuna pruriens (L.) DC.) based edible bioactive packaging formulation for antibacterial activity. Velvet bean ethanol extract (V) accompanied by cinnamon (Cinnamomum burmannii (Ness) BL) essential oil (C) which is effective as an antibacterial against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. Based on this study, the ascertained concentrations of V and C can generate the most optimum edible film associated with antibacterial activity and characterize the physical properties and morphology of the most optimum formulation of antibacterial edible film. It was proved that by addition of 30% V and 3.0% C, the most optimum edible film can be produced, which has maximum antibacterial activity against E. coli ATCC 25922 (19.36 mm strong) and S. aureus ATCC 25923 (18.94 mm strong). Moreover, this formulation boosts the thickness, tensile strength, and solubility of the film and simultaneously reduces the film's elongation, WVTR, and WVP. The edible bioactive packaging formulation produced thickness, tensile strength, solubility, percentage elongation, WVTR, and WVP values of 0.179 mm, 0.318 Mpa, 0.057%, 10.096%, and 3.747 g/m2.d, 8.586 g/m.d, respectively. The surface of the edible film still had some degree of porosity and texture, according to the morphology.
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[1] F. H. M. Saleh, A. Y. Nugroho, and M. R. Juliantama, “Pembuatan Edible Film dari Pati Singkong sebagai Pengemas Makanan,” Teknoin, vol. 23, no. 1, 2017, doi: https://doi.org/10.20885/teknoin.vol23.iss1.art5.
[2] A. Novelino, “Sampah Plastik 2021 Naik ke 11,6 Juta Ton, KLHK Sindir Belanja Online,” CNN Indonesia, 2022. [Online]. Available: https://www.cnnindonesia.com/nasional/20220225173203-20-764215/sampah-plastik-2021-naik-ke-116-juta-ton-klhk-sindir-belanja-online [accessed Mar. 11, 2024].
[3] C. Nurhanisah, Y; Devina, “Berapa Lama Sampah Plastik Bisa Terurai?,” IndonesiaBaik.id, 2023. [Online]. Available: https://indonesiabaik.id/infografis/berapa-lama-sampah-plastik-bisa-terurai. [accessed Mar. 14, 2024].
[4] M. Alpha, “Dampak Sampah Plastik Bagi Lingkungan Hidup,” Yayasan Bina Bhakti Lingkungan, 2022. [Online]. Available: https://www.yayasanbinabhaktilingkungan.or.id/dampak-sampah-plastik/. [accessed Mar. 11, 2024].
[5] Y. Taheri et al., “Paving Luteolin Therapeutic Potentialities and Agro‐Food‐Pharma Applications: Emphasis on In Vivo Pharmacological Effects and Bioavailability Traits,” Oxid. Med. Cell. Longev., vol. 2021, no. 1, p. 1987588, 2021.
[6] R. Utami, L. U. Khasanah, K. K. Yuniter, and G. J. Manuhara, “Pengaruh Oleoresin Daun Kayu Manis (Cinnamomum burmanii) DuaTahap terhadap Karakteristik Edible Film Tapioka,” Caraka Tani J. Sustain. Agric., vol. 32, no. 1, pp. 55–67, 2017, doi: 10.20961/carakatani.v32i1.15474.
[7] S. A. A. Mohamed, M. El-Sakhawy, and M. A.-M. El-Sakhawy, “Polysaccharides, Protein and Lipid-based Natural Edible Films in Food Packaging: A Review,” Carbohydr. Polym., vol. 238, p. 116178, 2020, doi: 10.1016/j.carbpol.2020.116178.
[8] L. U. Widodo, S. N. Wati, and N. M. Vivi, “Pembuatan Edible Film dari Labu Kuning dan Kitosan dengan Gliserol sebagai Plasticizer,” J. Teknol. Pangan, vol. 13, no. 1, pp. 59–65, 2019, doi: 10.33005/jtp.v13i1.151.
[9] F. Hayati, E. N. Dewi, and S. Suharto, “Karakteristik dan Aktivitas Antioksidan Edible Film Alginat dengan Penambahan Serbuk Spirulina platensis (Characteristic And Antioxidant Activity of Alginate Edible Film With The Addition Of Spirulina platensis Powder),” Saintek Perikan. Indones. J. Fish. Sci. Technol., vol. 16, no. 4, pp. 286–293, 2020, doi: 10.14710/ijfst.16.4.286-293.
[10] D. Huri and F. C. Nisa, “Pengaruh Konsentrasi Gliserol dan Ekstrak Ampas Kulit Apel terhadap Karakteristik Fisik dan Kimia Edible Film [In Press Oktober 2014],” J. pangan dan Agroindustri, vol. 2, no. 4, pp. 29–40, 2014, [Online]. Available: https://jpa.ub.ac.id/index.php/jpa/article/view/75. [accessed Mar. 12, 2024].
[11] S. R. D. Ariani, “Produksi Suplemen Antioksidan Alami Berbahan Baku Koro Benguk (Mucuna pruriens (L.) DC.) Pra-Fermentasi sebagai Peluang Usaha Baru di Era Pandemik,” in Seminar Nasional Kimia dan Pendidikan Kimia (SN-KPK), 2020, vol. 12, pp. 13–22. [Online]. Available: https://jurnal.uns.ac.id/snkpk/article/view/46966. [accessed Mar. 10, 2024].
[12] D. Yuniharni, “Uji Aktivitas Antibakteri Senyawa Flavonoid Total dan Tanin Total dari Ekstrak Daun Jambu Monyet (Anacardium occidentale. L),” Quim. J. Kim. Sains Dan Terap., vol. 3, no. 1, pp. 30–37, 2021, [Online]. Available: http://repositori.usu.ac.id/handle/123456789/21778. [accessed Mar. 10, 2024].
[13] R. Resianingrum, W. Atmaka, L. U. Khasanah, K. KAWIJI, R. Utami, and D. Praseptiangga, “Characterization of Cassava Starch-based Edible Film Enriched with Lemongrass Oil (Cymbopogon citratus),” Nusant. Biosci., vol. 8, no. 2, pp. 278–282, 2016, doi: 10.13057/nusbiosci/n080223.
[14] S. M. Rizki and R. S. Panjaitan, “Efektivitas Antifungi dari Minyak Atsiri Kulit Batang Kayu Manis (Cinnamommum burmanni) terhadap Candida albicans,” EduChemia (Jurnal Kim. dan Pendidikan), vol. 3, no. 2, pp. 172–183, 2018, doi: 10.30870/educhemia.v3i2.4560.
[15] S. R. D. Ariani, S. Nurul, and S. Falentina, “Isolation and Identification of Essential Oils from Bitter Ginger (Zingiber amaricans BL.), Fragrant Ginger (Zingiber aromaticum Val.) and Shampoo Ginger (Zingiber zerumbet (L.) Smith) RhizomesGrown in Jumapolo Karaganyar Central Java Indonesia,” J. Kim. Dan Pendidik. Kim, vol. 6, pp. 242–251, 2021, doi: 10.20961/jkpk.v6i2.48987.
[16] S. R. D. Ariani, L. Purniasari, U. Basyiroh, and E. Evangelista, “Chemical composition, antibacterial and antioxidant activities of essential oils from peels of four citrus species growing in Indonesia,” J. Essent. Oil Bear. Plants, vol. 25, no. 4, pp. 741–757, 2022.
[17] S. R. D. Ariani, S. Matsjeh, and B. Purwono, “QuantitativeAnalysis of L-Dopa (L-3, 4-dihydroxyphenylalanine) Content inExtracts of Various Products from Velvet Bean (Mucuna pruriens (L.) DC.) Tempe Processing,” Orient. J. Chem., vol. 32, no. 6, p. 2921, 2016, doi: 10.13005/ojc/320611.
[18] T. H. Kinasih, W. Sumarni, and E. B. Susatyo, “Pemanfaat Cangkang Kepiting Bakau Dan Plasticizer Gliserol Sebagai Edible Coating Buah Jambu Biji Merah,” J. MIPA, vol. 42, no. 1, pp. 7–15, 2019, [Online]. Available: http://journal.unnes.ac.id/nju/index.php/JM. [accessed Mar. 15, 2024].
[19] T. H. Kinasih, W. Sumarni, and E. B. Susatyo, “Pemanfaatan Cangkang Kepiting Bakau dan Plasticizer Ggliserol sebagai Edible Coating Buah Jambu Biji Merah,” Indones. J. Math. Nat. Sci., vol. 42, no. 1, pp. 7–15, 2019, [Online]. Available: http://journal.unnes.ac.id/nju/index.php/JM. [accessed Mar. 15, 2024].
[20] E. Kumalasari and E. Prihandiwati, “Pemanfaatan Limbah Daun Bawang Dayak (Eleutherine palmifolia L. Merr) sebagai Krim Anti Aging dengan Emulgator Anionik dan Nonionik,” J. Insa. Farm. Indones., vol. 2, no. 2, pp. 222–230, 2019, doi: 10.36387/jifi.v2i2.386.
[21] R. Dewi and E. Marniza, “Aktivitas Antibakteri Gel Lidah Buaya terhadap Staphylococcus aureus,” J. Saintek Lahan Kering, vol. 2, no. 2, pp. 61–62, 2019, doi: 10.32938/slk.v2i2.888.
[22] D. R. Sari and S. B. Prayitno, “Pengaruh Perendaman Ekstrak Bawang Putih (Allium Sativum) Terhadap Kelulushidupan dan Histologi Ginjal Ikan Lele (Clarias Gariepinus) yang Diinfeksi Bakteri ‘Edwardsiella Tarda,’” J. Aquac. Manag. Technol., vol. 3, no. 4, pp. 126–133, 2014, [Online]. Available: http://ejournal-s1.undip.ac.id/index.php/jamt. [accessed Mar. 9, 2024].
[23] D. Molnar, D. Novotni, M. Kurek, D. Iveković, H. Bionda, and M. Ščetar, “Characteristics of Edible Films Enriched with Fruit by Products and Their Application on Cookies,” Food Hydrocoll., vol. 135, p. 108191, 2023, doi: 10.1016/j.foodhyd.2022.108191.
[24] N. I. F. Wahab, F. Y. P. Tyassena, and F. Junianti, “Pembuatan Edible Film Berbahan Baku Karagenan dengan Variasi Suhu Pemanasan dan Konsentrasi Gliserol,” Jtkm, vol. 2, no. 2, pp. 98–102, 2023, doi: 10.61844/jtkm.v2i2.676.
[25] Z. K. Ayuningrum, “Pengaruh Minyak Atsiri Kulit Batang Kayu Manis (Cinnamomum burmanii) terhadap Karakteristik Edible Film dan Kualitas Daging Sapi Segar (Skripsi),” 2015.
[26] A. Taqi, K. A. Askar, K. Nagy, L. Mutihac, and L. Stamatin, “Effect of Different Concentrations of Olive Oil and Oleic Acid on the Mechanical Properties ofAlbumen (Egg White) Edible Films,” African J. Biotechnol., vol. 10, no. 60, pp. 12963–12972, 2011, doi: 10.5897/AJB11.1971.
[27] Y. Afriyah, W. D. R. Putri, and S. D. Wijayanti, “Penambahan Aloe vera L. dengan Tepung Sukun (Artocarpus communis) dan Ganyong (Canna edulis Ker.) terhadap Karakteristik Edible Film [In Press September 2015],” J. Pangan dan Agroindustri, vol. 3, no. 4, 2015, [Online]. Available: https://jpa.ub.ac.id/index.php/jpa/article/view/254. [accessed Mar. 10, 2024].
[28] J. M. Krochta and C. de Mulder-Johnston, “Edible and Biodegradable Polymer Films: Challenges and Opportunities.,” 1997, [Online]. Available: https://search.worldcat.org/en/title/45381874. [accessed Mar. 14, 2024].
[29] R. Utami, L. U. Khasanah, K. K. Yuniter, and G. J. Manuhara, “Pengaruh Oleoresin Daun Kayu Manis (Cinnamomum burmanii) Dua Tahap terhadap Karakteristik Edible Film Tapioka,” Caraka Tani J. Sustain. Agric., vol. 32, no. 1, p. 55, 2018, doi: 10.20961/carakatani.v32i1.15474.
[30] R. Arham, M. T. Mulyati, M. Metusalach, and S. Salengke, “Physical and Mechanical Properties of Agar based Edible Film with Glycerol Plasticizer.,” Int. Food Res. J., vol. 23, no. 4, 2016, [Online]. Available: http://www.ifrj.upm.edu.my/. [accessed Mar. 18, 2024].
[31] R. Dwimayasanti and B. Kumayanjati, “Karakterisasi Edible Film dari Karagenan dan Kitosan dengan Metode Layer by Layer,” J. Pascapanen dan Bioteknol. Kelaut. dan Perikan., vol. 14, no. 2, p. 141, 2019, doi: 10.15578/jpbkp.v14i2.603.
[32] S. Benavides, R. Villalobos-Carvajal, and J. E. Reyes, “Physical, Mechanical and Antibacterial Properties of Alginate Film: Effect of the Crosslinking Degree and Oregano Essential Oil Concentration,” J. Food Eng., vol. 110, no. 2, pp. 232–239, 2012, doi: 10.1016/j.jfoodeng.2011.05.023.
[33] L. H. Cheng, A. Abd Karim, M. H. Norziah, and C. C. Seow, “Modification of the Microstructural and Physical Properties ofKonjac Glucomannan-based Films by Alkali and Sodium Carboxymethyl cellulose,” Food Res. Int., vol. 35, no. 9, pp. 829–836, 2002, doi: 10.1016/S0963-9969(02)00086-8.
[34] P. Alonso, S. F. Pastor., and A. Guerrero, "Application of Cinnamon Essential Oil in Active Food Packaging: A Review," Applied Sciences, vol. 14, no. 15, pp. 1-19, 2024. doi: 10.3390/app14156554.
[35] F. Yulistiani, D. R. D. Kurnia, M. Agustina, and Y. Istiqlaliyah, “Pembuatan Edible Film Antibakteri Berbahan Dasar Pektin Albedo Semangka, Sagu, dan Ekstrak Bawang Putih,” Fluida, vol. 12, no. 1, pp. 29–34, 2019, doi: 10.35313/fluida.v12i1.1621.
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