The Antibacterial Activity of Liquid Soap supplemented with Extracts combination of Cyperus rotundus L. and Flowers of Plumeria acuminata, Michelia alba, or Cananga odorata Against Staphylococcus aureus and Escherichia coli Bacteria

Sri Mulyani, Ari Kusumawardani, Aulia Ageng Pangesti

Abstract

Secondary metabolites found in white Frangipani flowers (Plumeria acuminata), white tropical Magnolia flowers (Michelia alba), Ylang flower (Cananga Odorata), and Nutgrass (Cyperus rotundus L.) are thought to be employed as antibacterial active ingredients in detergent. This research aims to create liquid antibacterial soap using an extract mixture of Nutgrass with white Frangipani flower (F1), white tropical Magnolia flower (F2), or Ylang flower (F3). Phytochemical screening tests for the ethanol extracts of Nutgrass and those three flowers, pH tests, total active ingredients tests, and antibacterial activity tests against S. aureus and E. coli bacteria were carried out during the formulation of the liquid soap preparation with the addition of these extracts. The extraction procedure was maceration with a 70% ethanol solvent, yielding extracts of 9.28 percent frangipani flower, 8.12 percent white tropical Magnolia flowers, 9.8 percent Ylang flower, and 8.88 percent Nutgrass. The liquid soap quality testing findings met the established norms (SNI 4085:2017). This research shows that the soap formulas (F0, F1, F2, and F3) can inhibit S. aureus and E. coli germs in antibacterial activity tests using the disc diffusion method. Based on the diameter of the inhibition zone, the antibacterial activity against E. coli of liquid soap with Nutgrass & white tropical Magnolia flower extracts (F2) is not significantly different from liquid soap without extracts (F0). The F2 formula shows to be less effective than both the two liquid soaps containing extracts of Nutgrass & white Frangipani flower (F1) and Nutgrass & Ylang flower (F3). However, the three formulas of liquid soaps (F1, F2, and F3) tend to be more effective in antibacterial activity against S. aureus than a liquid soap that did not contain extracts (F0).

Keywords

liquid soap, antibacterial, white frangipani flower, white tropical magnolia's flower, nutgrass

Full Text:

PDF

References

[1] M. R. Chirani, E. Kowsari, T. Teymourian, & S. Ramakrishna, Environmental impact of increased soap consumption during COVID-19 pandemic: Biodegradable soap production and sustainable packaging. Science of The Total Environment, Vol 796, 2021.

[2] S. Akita, M. Fujioka, T. Akita, J. Tanaka, A. Masunaga, & T. Kawahara, "Effects of hand hygiene using 4% chlorhexidine gluconate or natural soap during hand rubbing followed by alcohol-based 1% chlorhexidine gluconate sanitizer lotion in the operating room". Advances in Wound Care, vol.11, No.1,pp. 1-9,2022.

[3] A. Widyasanti, A. S.Septianur, & S.Rosalinda, (2019). Liquid Soap Making From Castor Oil With Various Infused Oil With Tea Consentrations. JTIP Indonesia, vol.11 no.1, pp. 11–18,2019.

[4] Sari, R., & Ferdinan, A. Pengujian Aktivitas Antibakteri Sabun Cair dari Ekstrak Kulit Daun Lidah Buaya. Pharm Sci, vol.4, no 3, pp. 111–120, 2017

[5] Moningka, M. V, Pareta, D., & Potalangi, N. (). Formulasi dan Uji Aktivitas Antibakteri Sediaan Sabun Cair Ekstrak Daun Pala Myristica fragrans Houtt. Jurnal Biofarmasetikal Tropis, vol.3, no 2,pp. 17–26, 2020

[6] R. M. Putra, A. Fahrurroji, & B. Wijayanto, Optimasi Formulasi Sabun Mandi Cair Ekstrak Etanol Rimpang Jahe Merah (Zingiber Officinale Rosc. Var Rubrum) Dengan Metode Simplex Lattice Design. Jurnal Teknosains, vol.5, no.2, pp. 81–116, 2016.

[7] W. S. Rita, N. P. E.Vinaprilliani, & I. W. G. Gunawan, “Formulasi Sediaan Sabun Padat Minyak Atsiri Serai Dapur (Cymbopogon citratus DC.) Sebagai Antibakteri Terhadap Escherichia coli dan Staphylococcus aureus”. Cakra Kimia (Indonesian E-Journal of Applied Chemistry, vol.6, no.2,pp. 152–160. 2018

[8] L. Handayani, “Pengaruh Kandungan Deterjen Pada Limbah Rumah Tangga Terhadap Kelangsungan Hidup Udang Galah (Macrobracium Rosenbergii). Sebatik, vol.24. nop.1,pp. 75–80, 2020.

DOI:10.46984/sebatik.v24i1.937

[9] A. Widyasanti, A. T. Winaya, & S. Rosalinda,” Pembuatan Sabun Cair Berbahan Baku Minyak Kelapa Dengan Berbagai Variasi Konsentrasi Ekstrak Teh Putih”. Agrointek, vol.13,no.2,pp. 132–142,2019. DOI:10.21107/agrointek.v13i2.5102

[10] Dieringer, G., Cabrera R, L., Lara, M., Loya, L., & Reyes-Castillo, P. (1999). Beetle pollination and floral thermogenicity in Magnolia tamaulipana (Magnoliaceae). International Journal of Plant Sciences, 160(1), 64-71.

[11] D.Y. Reyna, and J. A.V.-García. "Flower of the heart, Magnolia yajlachhi (subsect. Talauma, Magnoliaceae), a new species of ceremonial, medicinal, conservation and nurse tree relevance in the Zapotec culture, Sierra Norte de Oaxaca, Mexico." Phytotaxa vol.393, no.1,pp. 21-34,2019.

[12] V. Shanmugam, S. Manoharan, , S. Anandan, , & Murugan, "Performance of dye-sensitized solar cells fabricated with extracts from fruits of ivy gourd and flowers of red frangipani as sensitizers". Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol.104,pp. 35-40,2013.

[13] S. Mulyani, K. S. Aristia, A. P.Sabrina, A. Arfiah, & B. Niam, "Potential of Water Extract of The White Frangipani (Plumeria acuminate) and Hibiscus (Hibiscus tiliaceus) Leaves Powder as Textile Natural Dyes". JKPK (Jurnal Kimia dan Pendidikan Kimia), vol.5, no.1,pp. 100-109, 2020.

[14] R. Pujiarti, T. B. Widowati, Kasmudjo, & S. Sunarta, “Kualitas, Kompoisis Kimia, dan Aktivitas Antioksidan Minyak Kenanga (Cananga odorata),” J. Ilmu Kehutan., vol. 9, no. 1, pp. 3–11, 2015.

[15] J. Jin et al., “The floral transcriptome of ylang ylang (Cananga odorata var. fruticosa) uncovers biosynthetic pathways for volatile organic compounds and a multifunctional and novel sesquiterpene synthase,” J. Exp. Bot., vol. 66, no. 13, pp. 3959–3975, 2015, doi: 10.1093/jxb/erv196.

[16] M. Brokl, M. L. Fauconnier, C. Benini, G. Lognay, P. Du Jardin, & J. F. Focant, “Improvement of ylang-ylang essential oil characterization by GC×GC-TOFMS,” Molecules, vol. 18, no. 2, pp. 1783–1797, 2013, doi: 10.3390/molecules18021783.

[17] L. T. H. Tan et al., “Traditional uses, phytochemistry, and bioactivities of Cananga odorata (ylang-ylang),” Evidence-based Complement. Altern. Med., vol. 2015, pp. 1–30, 2015, doi: 10.1155/2015/896314.

[18] F. Rahim, C. Deviarny, R. Yenti, & P. Ramadani, “ari rimpang rumput teki (Cyperus rotundus L.) untuk pengobatan nyeri sendi pada tikus putih jantan”. SCIENTIA: Jurnal Farmasi dan Kesehatan, vol.6, no.1,pp. 1-6, 2016.

[19] O. M. Oladunni, O. O.Abass, & A. I. Adisa, "Studies on physicochemical properties of the Oil, Minerals and Nutritional composition of Nut of Nutgrass (Cyperus rotundus)". American Journal of Food Technology, vol.6, no.12, pp. 1061-1064, 2016.

[20] D.Balarak,H. Mardanshahi, K. Chandrika, & C.S. Felice, "Thermodynamic Studies of the Removal of Lead from Synthetic Wastewater using Cyperus rotundus (Nut grass)". Oriental Journal of Chemistry, vol.35, no.5, pp. 1529-1534, 2019.

[21] L. R. Dita, , & J.Triastuti, "Utilization of agar Gracilaria sp. as a natural thickener on liquid bath soap formulation". In IOP Conference Series: Earth and Environmental Science Vol. 441, No. 1, p. 01 2021. IOP Publishing.

[22] E.Gyedu-Akoto, D. Yabani, J. Sefa, & D. Owusu, "Natural skin-care products: the case of soap made from cocoa pod husk potash". Advances in Research, vol.4.no.6, pp. 365-370,2015.

[23] C. Nurhayati, N.Susilawati, T. Susanto, W. Marthalia, & A. K. Nugroho, "Utilization of gambier leaf extract (Uncaria gambier roxb) as antibacterial and natural dyes in making liquid bath soap with natrium lauryl ether sulphate". In IOP Conference Series: Earth and Environmental Science Vol. 963, No. 1, p. 012059,2022. IOP Publishing.

[24] S.A Ariyani & H. Hidayati, " Penambahan Gel Lidah Buaya Sebagai Antibakteri Pada Sabun Mandi Cair Berbahan Dasar Minyak Kelapa. Jurnal Industri Hasil Perkebunan, vol. 13, no.1,pp. 11–18. 2018

[25] E. B. Minarno, “ Skrining Fitokimia dan Kandungan Total Flavanoid Pada Buah Carica pubescens Lenne & K. Koch di Kawasan Bromo, Cangar, dan Dataran Tinggi Dieng”. El-Hayah, vol. 5, no.2,pp. 73–82, 2015. DOI:10.4269/ajtmh.1986.35.167

[26] S. P.Pasaribu, W.Nuriah, & Erwin. “ Uji Toksisitas dan Aktivitas Antibakteri Berbagai Fraksi Ekstrak Daun Tanaman Kamboja (Plumeria acuminate Ait.)”. Jurnal Kimia Mulawarman, vol.10,no.2,pp 94–99,2013.

[27] F. C. Korompis,. P. V. Y. Yamlean, & W. A. Lolo, “ Formulasi dan Uji Efektivitas Antibakteri Sediaan Sabun Cair Ekstrak Etanol Daun Kersen (Muntingia Calabura L.) Terhadap Bakteri Staphlococcous epidermidis. Pharmacon, vol.9, no.1, pp. 30–37,2020. https://doi.org/10.35799/pha.9.2020.27407

[28] N. N. Rupiniasih, I. Syamsuddin, & A. R. Razak, “ Aktivitas Antibakteri Fraksi N-Heksan, Kloroform, Etil Asetat Bunga Kamboja (Plumeria Alba) Terhadap Bakteri Staphylococcus Aureus dan Salmonella typhi. KOVALEN: Jurnal Riset Kimia, vol.5, no.2,pp 173–181,2015.

[29] Sulistyarsi, A., & Cahyani, F. M. (2018). Potensi Ekstrak Daun Kamboja Putih dari Madiun dan Magetan Sebagai Penghambat Pertumbuhan Bakteri Salmonella Typhosa (In Vitro). Seminar Nasional Hasil Penelitian Dan Pengabdian Kepada Masyarakat UNIPMA, UNIPMA, 219–227.

[30] M. A. Husni, H. Muriana, Helwati, , & Nuraini. Antimicrobial Activity of N-Hexane Extracts of Red Frangipani (Plumeria rocea). Jurnal Natural, vol.13, no.1, 28–33,2013.

[31] A. J.Pananginan, V. Hariyadi, Paat, , & Y. Saroinsong, (). Formulasi dan Uji Aktivitas Antibakteri Sediaan Sabun Cair Ekstrak Daun Jarak Tintir (Jatropha Multifidi L.). Jurnal Biofarmasetikal Tropis, vol.3, no.1, 148–158, 2020.

[32] R. Rinaldi, F. Fauziah, & R. Mastura, “Formulasi dan Uji Daya Hambat Sabun Cair Ekstrak Etanol Serai Wangi (Cymbopogon Nardus L) Terhadap Pertumbuhan Staplylococcus aureus”. Jurnal Riset Kefarmasian Indonesia, vol.3,no.1, 45–57, 2021. https://doi.org/10.33759/jrki.v3i1.115

[33] S. A. Dimpudus, P. V. Y.Yamlean, & A.Yudistira, “Formulasi Sediaan Sabun Cair Antiseptik Ekstrak Etanol Bunga Pacar Air (Impatiens Balsamina L.) dan Uji Efektivitasnya Terhadap Bakteri Staphylococcus Aureus Secara In Vitro”. In PHARMACONJurnal Ilmiah Farmasi-UNSRAT Vol. 6, no.3, 2017.

[34] A.Widyasanti, & J. M. Rohani, “Pembuatan Sabun Padat Transparan Berbasis Minyak Zaitun Dengan Penambahan Ekstrak Teh Putih. Jurnal Penelitian Teh Dan Kina, vol.20,no.1,pp. 13–29, 2017.

[35] T. S. Julianto, Minyak Atsiri Bunga Indonesia, ISBN 978-602-401-205-2, 2016, Yogyakarta: Deeppublish.

[36] R., Paramitha, Athaillah, R. Rambe, & Selvina. “Pengujian Aktivitas Antibakteri Sabun Cair dari Ekstrak Etanol Buah Pepaya (Carica Papaya L) Pada Bakteri Staphylococcus aureus. Forte Journal, vol.1,no.1, pp.12–18, 2021.

[37] A. W.Soehendro, G. J. Manuhara & E. Nurhartadi,” Pengaruh Suhu Terhadap Aktivitas Antioksidan dan Antimikrobia Ekstraksi Biji Melinjo ( Gnetum gnemon L .) Dengan Pelarut Etanol dan Air”. Jurnal Teknosains Pangan, vol.4 no.4,pp. 15–24, 2015.

[38] A. Z. Robbia, Y. Yahdi, & Y. K. Dewi, Perbandingan Pengaruh Ekstrak Lidah Buaya (Aloe vera) dan Ekstrak Daun Sirih (Piper betle Linn) Terhadap Kualitas Produk Hand Soap. Jurnal Pijar Mipa, 16(2), 228–234. 2021, DOI:10.29303/jpm.v16i2.2452.

[39] P. Y.Irmayanti, N. P. A. D Wijayanti,& C. I. S. Arisanti, “Sediaan Sabun Mandi Cair dari Ekstrak Kulit Manggis (Garcinia Mangostana Linn.)”. Jurnal Kimia, vol.8, no.2, pp. 237–242, 2014.

[40] D. W. Lestari, V. Atika, A.Isnaini, Haerudin, & T.K. Arrta, “Pengaruh pH Ekstraksi Pada Pewarnaan Batik Sutera Menggunakan Pewarna Alami Kulit Kayu Mahoni (Switenia Mahagoni”). Jurnal Rekayasa Proses vol.14, no.1, pp. 74–81,2020.

DOI: 10.22146/jrekpros.54439


Refbacks

  • There are currently no refbacks.