Synthesin and Characterization Chitosan Film with Silver Nanoparticle Addition As A Multiresistant Antibacteria Material

Endang Susilowati, Sri Retno Dwi Ariani, Lina Mahardiani, Luthfia Izzati


The synthesis of chitosan films with the addition of silver nanoparticles (AgNps) has been carried out in 2 stages. The first stage is to make colloidal silver nanoparticles with chitosan as a stabilizer and a reducing agent with NaOH as an accelerator and assisted by microwave irradiation. The second stage is to make a film from colloidal silver nanoparticles-chitosan by casting method. The formation of silver nanoparticles was identified using a UV-Vis spectrophotometer and TEM. Chitosan films with the addition of AgNps were characterized by chemical, physical and mechanical properties. Furthermore, the antibacterial activity was tested against multi-resistant bacteria ESBL and MRSA. The results showed that the formation of AgNps was indicated by the appearance of an absorption band at 400-413 nm with a size of less than 6 nm. The FTIR spectra showed that there was a slight shift at the 1604 cm-1 peak which indicated that the AgNps interacted with the NH2 group on D-glucosamine of chitosan. The films morphology with addition Ag NPs tends to be rough in surface and cross-sectional. The presence of AgNps tends to increase the swelling value, tensile strength and film elasticity. Chitosan film with the addition of silver nanoparticles has antibacterial activity against multi-resistant bacteria ESBL and MRSA. The anti-bacterial activity of the film was derived solely from the silver nanoparticles.


Chitosan film; silver Nanoparticles; antibacterial; multiresistant

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[1] S. W. Ngan, Vanessa., “Honey in wound care,” 2005.
Available: google scholar

[2] Y. J. Shahidi, F., Arachchi, J. K. V., and Jeon, “Food Application of Chitin and Chitosan,” Trend Food Sci. Technol., vol. 10, pp. 37–51, 1999.

[3] F. Prisiska, “The Influence of Chitosan on Elongation and Tensile Strength Characteristic of Wound Healing Biomembrane Abstract,” pp. 1–14, 2010.
Available: google scholar

[4] B. Balicka-ramisz, A., Wojtasz-pajak, A., & Pilarczyk, “Antibacterial and antifungal activity of chitosan,” Science (80-. )., vol. 2, pp. 406–408, 2005.
Available: google scholar

[5] R. Kalaivani et al., “Synthesis of chitosan mediated silver nanoparticles (Ag NPs) for potential antimicrobial applications,” Front. Lab. Med., vol. 2, no. 1, pp. 30–35, 2018.

[6] K. Renuka, A. Kapil, S. Kabra, and E. Al., “Reduced susceptibility to ciprofloxacin and gyra gene mutation in north Indian strains of Salmonella enterica serotype Typhi and serotype Paratyphi A,” vol. 10, no. Microb Drug Resist, pp. 146–153, 2004.
DOI: 10.1089/1076629041310028

[7] S. Chakraborty, J. Deokule, and et al Garg P, “Concomitant infection of enterotoxigenic Escherichia coli in an outbreak of cholera caused by Vibrio cholerae O1 and O139 in Ahmedabad, India,” vol. 7, no. J Clin Microbiol, pp. 514–516, 2001.
DOI: 10.1128/JCM.39.9.3241-3246.2001

[8] S. T. Odonkor and K. K. Addo, “Review article Bacteria Resistance to Antibiotics : Recent Trends and Challenges,” vol. 2, no. 4, pp. 1204–1210, 2011.
Available: google scholar

[9] S. Uzunović et al., “Meticilin-rezistentni S. Aureus (MRSA) i gram-negativne bakterije koje proizvode ß-laktamaze proširenog spektra (ESBL) i plazmidom-posredovane AmpC ß-laktamaze kao uzročnici bolničkih i vanbolničkih infekcija kože i mekih tkiva,” Med. Glas., vol. 12, no. 2, pp. 157–168, 2015.

[10] S. F. G. El-Kheshen, A. A., & El-Rab, “Effect of reducing and protecting agents on size of silver nanoparticles and their anti-bacterial activity,” J. Mater. Sci., vol. 4 (1), no. Materials in Medicine, pp. 53–65, 2012.
Available: google scholar

[11] C. S. Cao, X. L., Cheng, C., Ma, Y. L., & Zhao, “Preparation of silver nanoparticles with antimicrobial activities and the researches of their biocompatibilities,” J. Mater. Sci., vol. 21(10), no. Materials in medicine, pp. 2861–2868, 2010.
DOI: 10.1007/s10856-010-4133-2

[12] V. Bozanic, D.K., Dmitrijevic-Brankovic, S., Bibic, N., Luyt, A. S., Djokovic, “Silver Nanoparticles Encapsulated in Glycogen Biopolymer: Morphology, Optical and AntimicrobialProperties,” no. Carbohydrate Polymers, 2011.

[13] K. Darraoudi, M., Ahmad, M.B., Abdullah A.H., Ibrahim, N.A., Shameli, “Effect of Accelerator in Green Synthesis of SilverNanoparticles,” Int. J. Mol. Sci., vol. 11, pp. 3898–3905, 2010.
doi: 10.3390/ijms11103898

[14] H. A. Ariyanta, “Silver Nanoparticles Poration by Reduction Method and Its Application as Antibacterial for Cause of Wound Infection,” J. MKMI, pp. 36–42, 2014.
DOI: 10.30597/mkmi.v10i1.477

[15] M. Y. Ahmad, M.B., Lim, J.J., Shameli, K., Ibrahim, N.A., Tay, “Synthesis of Silver Nanoparticles in Chitosan, Gelatin and Chitosan/Gelatin Bionanocomposites by a Chemical Reducing Agent and Their Characterization,” vol. 16, no. Molecules, pp. 7232–7248, 2011.
doi: 10.3390/molecules16097237

[16] V. Bozanic, D.K., Trandafilovic, L.V., Luyt, A.S., Djokovic, “Green Synthesis and Optical Properties of Silver-Chitosan Complexes and Nanocomposites,” vol. 70, no. Reactive and Fuctional Polymer, pp. 869–873, 2010.

[17] S. Endang, Maryani, Ashadi, and U. Hasanah, “SINTESIS KITOSAN HIDROLISAT SECARA ENZIMATIS MENGGUNAKAN ENZIM PAPAIN,” Semin. Nas. Kim. DAN Pendidik. Kim. VII, 2015.
Available: google scholar

[18] G. B. Ortiz-Duartea, G., Perez-Cabrerab, L.E., Artes-Hernandezc, F., Martinez-Hernandezc, “Ag-Chitosan Nanocomposites in Edible Coatings Affect The Quality of Fresh-cut Melon,” Postharvest Biol. ang Technol., vol. 147, pp. 174–184, 2018.

[19] T. Wahyudi, D. Sugiyana, and Q. Helmy, “Sintesis Nanopartikel Perak dan Uji Aktivitasnya terhadap Bakteri E.coli dan S.aureus,” Balai Besar Tekst., vol. 26, no. 1, pp. 55–60, 2011.

[20] E. Susilowati, T. Triyono, Santosa, and Kartini, “Synthesis of Silver-Chitosan Nanocomposites Colloidal by Glucose as Reducing Agent,” Indones. J. Chem., vol. 15(1), pp. 29–35.

[21] E. Susilowati, Maryani, Ashadi, and Marwan, “Fabrication of silver-chitosan nanocomposite films and their antibacterial activity,” IOP Conf. Ser. Mater. Sci. Eng., vol. 858, no. 1, 2020.

[22] M. Rai, A. Yadav, and A. Gade, “Silver nanoparticles as a new generation of antimicrobials,” Biotechnol. Adv., vol. 27, no. 1, pp. 76–83, 2009.


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