Synthesin and Characterization Chitosan Film with Silver Nanoparticle Addition As A Multiresistant Antibacteria Material

Endang Susilowati, Sri Retno Dwi Ariani, Lina Mahardiani, Luthfia Izzati

Abstract

The synthesis of chitosan films with the addition of silver nanoparticles (AgNps) has been carried out in 2 stages. The first stage is to make colloidal silver nanoparticles with chitosan as a stabilizer and a reducing agent with NaOH as an accelerator and assisted by microwave irradiation. The second stage is to make a film from colloidal silver nanoparticles-chitosan by casting method. The formation of silver nanoparticles was identified using a UV-Vis spectrophotometer and TEM. Chitosan films with the addition of AgNps were characterized by chemical, physical and mechanical properties. Furthermore, the antibacterial activity was tested against multi-resistant bacteria ESBL and MRSA. The results showed that the formation of AgNps was indicated by the appearance of an absorption band at 400-413 nm with a size of less than 6 nm. The FTIR spectra showed that there was a slight shift at the 1604 cm-1 peak which indicated that the AgNps interacted with the NH2 group on D-glucosamine of chitosan. The films morphology with addition Ag NPs tends to be rough in surface and cross-sectional. The presence of AgNps tends to increase the swelling value, tensile strength and film elasticity. Chitosan film with the addition of silver nanoparticles has antibacterial activity against multi-resistant bacteria ESBL and MRSA. The anti-bacterial activity of the film was derived solely from the silver nanoparticles.

Keywords

Chitosan film; silver Nanoparticles; antibacterial; multiresistant

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