Synthesis of Nano Silver using Bioreductor of Tristaniopsis merguensis Leaf Extracts and Its Antibacterial Activity Test

Verry Andre Fabiani, Megawati Ayu Putri, Marhan Ebit Saputra, Della Puspita Indriyani

Abstract

Synthesis nanosilver from pelawan leaf extract (Tristaniopsis merguensis) has been carried out. The variables of this study were variations in the concentration of AgNO3 1 mM, 1.5 mM and 2 mM. Pelawan leaf extract acted as a bioreductor that converts Ag+ to Ag0. The synthesis was carried out in a ratio of 1: 4 (opposition leaf extract: AgNO3 solution) at 70 °C for 1.5 hours. The results of the UV-Vis analysis showed the maximum at the 1 mM; 1.5 mM; 2 mM AgNO3 concentration were 391 nm, 392 nm and 400 nm, respectively. XRD analysis explained that the resulting nanosilver was crystalline and according the Scherrer equation an average particle size was of 22.8 nm. The antibacterial activity test of nanosilver was carried out by disc method, nanosilver showed the existence of strong antibacterial activity against E. coli and S. aureus bacteria.

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Keywords

antibacterial; pelawan leaf; particle size; nanosilver

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References

H. A. Ariyanta & S. Wahyuni, “Preparasi Nanopartikel Perak dengan Metode Reduksi dan Aplikasinya Sebagai Antibakteri Penyebab Infeksi,” Indones. J. Chem. Sci., vol. 3, no. 1, pp. 1–6, 2014.

A. Haryono & S. B. Harmami, “Aplikasi Nanopartikel Perak pada Serat Katun sebagai Produk Jadi Tekstil Antimikroba,” J. Kim. Indones., vol. 5, no. 1, pp. 1–6, 2010.

Z. Xiu, Q. Zhang, H. L. Puppala, V. L. Colvin, & P. J. J. Alvarez, “Negligible Particle-Specific Antibacterial Activity of Silver Nanoparticles,” Nano Lett., vol. 12, pp. 4271–4275, 2012.

N. Yarli, “Ekologi pohon pelawan (Tristaniopis merguensis Griff.) sebagai inang jamur pelawan di Kabupaten Bangka Tengah,” IPB, 2011.

L. Verotta, M. D. Agli, A. Giolito, M. Guerrini, P. Cabalion, & E. Bosisio, “In Vitro Antiplasmodial Activity of Extracts of Tristaniopsis Species and Identification of the Active Consti-tuents : Ellagic Acid and 3 , 4 , 5-Trimethoxyphenyl-(6′-O-galloyl )- O-B-D-glucopyranoside,” J. Nat. Prod., vol. 64, no. 5, pp. 603–607, 2001.

V. A. Fabiani, F. Sutanti, D. Silvia, & M. A. Putri, “Green Synthesis Nano-partikel Perak Menggunakan Ekstrak Daun Pucuk (Idat (Cratoxlyum glaucum) sebagai Bioreduktor,” Indo. J. Pure App. Chem, vol. 1, no. 2, pp. 68–76, 2018.

M. Julkarnain, A. K. Mondal, M. Rahman, & S. Rana, “Preparation and Properties of Chemically Reduced Cu and Ag Nanoparticles,” in Inter-national Conference on Mechanical, Industrial and Materials Engineering, 2013, vol. 2013, pp. 636–640.

S. K. Ghosh, S. Kundu, M. Mandal, & T. Pal, “Silver and Gold Nanocluster Catalyzed Reduction of Methylene Blue by Arsine in a Micellar Medium,” Langmuir, vol. 18, no. 23, pp. 8756–8760, 2002.

Y. Masakke, Sulfikar, & M. Rasyid, “Biosintesis Partikel-nano Perak Menggunakan Ekstrak Metanol Daun Manggis ( Garcinia mangostana L . ),” J. Sainsmat, vol. IV, no. 1, pp. 28–41, 2015.

S. Arief, W. Rahma, D. V. Wellia, & Zulhadjri, “Green Synthesis Nano-partikel Ag dengan Menggunakan Ekstrak Gambir sebagai Bioreduktor,” in SEMIRATA 2015 Bidang MIPA BKS-PTN Barat, 2015, pp. 233–238.

M. D. Purnamasari, “Sintesis Anti-bakteri Nanopartikel Perak Mengguna-kan Bioreduktor Ekstrak Daun Sirih (Piper betle Linn) dengan Iradiasi Microwave,” 2015.

E. Y. Lembang & M. M. Zakir, “Sintesis Nanopartikel Perak dengan Metode Reduksi Menggunakan Bio-reduktor Ekstrak Daun Ketapang (Terminalia catappa),” Universitas Hasanuddin, 2013.

T. Wahyudi, D. Sugiyana, & Q. Hemly, “Sintesis Nanopartikel Perak dan Uji Aktivitasnya Terhadap Bakteri E. coli dan S. aureus,” Arena Tekst., vol. 26, no. 1, pp. 55–60, 2011.

K. N. Thakkar, S. S. Mhatre, & R. Y. Parikh, “Biological synthesis of metallic nanoparticles,” Nanomedicine Nano-technology, Biol. Med., vol. 6, no. 2, pp. 257–262, 2010.

V. Kumar & S. K. Yadav, “Plant-mediated synthesis of silver and gold nanoparticles and their applications,” J Chem Technol Biotechnol, vol. 84, no. June 2008, pp. 151–157, 2009.

W. R. O. Yanti & Astuti, “Sintesis Nanokristal Perak Menggunakan Ekstrak Kulit Buah Manggis (Garcinia mangostana L.),” J. Fis. Unand, vol. 7, no. 3, pp. 286–291, 2018.

S. Jain & M. S. Mehata, “Medicinal Plant Leaf Extract and Pure Flavonoid Mediated Green Synthesis of Silver Nanoparticles and their Enhanced Antibacterial Property,” Sci. Rep., vol. 7, no. November, pp. 1–13, 2017.

S. D. Solomon, M. Bahadory, A. V Jeyarajasingam, S. A. Rutkowsky, & C. Boritz, “Synthesis and Study of Silver Nanoparticles,” J. Chem. Educ., vol. 84, no. 2, pp. 322–325, 2007.

S. Nikmatin, A. Maddu, S. Purwanto, T. Mandang, & A. Purwanto, “Analisa Struktur Mikro Pemanfaatan Limbah Kulit Rotan Menjadi Nanopartikel Selulosa Sebagai Pengganti Serat Sintesis,” J. Biofisika, vol. 6, no. 1, pp. 41–49, 2013.

W. Davis & T. Stout, “Disc Plate Method of Microbiological Antibiotic Assay,” Appl. Microbiol., vol. 22, no. 4, pp. 659–665, 1971.

B. Biswas, K. Rogers, F. Mclaughlin, D. Daniels, & A. Yadav, “Anti-microbial Activities of Leaf Extracts of Guava (Psidium guajava L .) on Two Gram-Negative and Gram-Positive Bacteria,” Int. J. Microbiol., vol. 2013, pp. 1–7, 2013.

DOI: https://doi.org/10.20961/jkpk.v4i3.34617

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