ANTIBACTERIAL ACTIVITY OF XANTHONE FROM ETHYL ACETATE EXTRACT OF THE STEAM BARK OF Garcinia picrorrhiza Miq

Muharni Muharni, Elfita Elfita, Emil Pertiwi

Abstract

A compound was isolated from the ethyl acetate extract of stem bark Garcinia picrorrhiza. The extraction was conducted by maseration. Separation and purification were done by chromatography method. The structure of compound was established using UV, IR, and 1H NMR spectroscopy. The antibacteria activity of the isolated compound was tested by paper disk difusion method and minimum inhibitory concentration (MIC)   value was determined by using well difusion method examined against bacteria Escherechia coli, Salmonella typhi, Bacillus subtilis and Staphyloccocus aureus. The isolated compound was a yellow solids (43.8 mg) with melting point 171 – 172 oC. Based on spectroscopy data compared with data from the literature, the isolated  compound is a known compound of oxygenated xanthone group with structure 1,4,5-trihydroxy-2-(3methylbut-2-enyl)xanthone. The compound exhibited an antibacterial activity against Bacillus subtilis only with MIC of 62.5 µg/mL.

Keywords

antibacterial; Garcinia picrorrhiza Miq; xanthone

Full Text:

PDF

References

Astuti, M. D., and Taslim, E., 2009. Senyawa Fenolat dari Kayu Batang Garcinia picrorrohiza Miq. Jurnal Saint dan Tetapan Kimia 2(1), 94-103.

Bennett, G. J., and Lee, H. H., 1989. Xanthones from Guttiferae. Phytochemistry 28 (4), 967-998.

Chandra, R., Vinay, D., Abhimanyu, K. J., and Kumar, S., 2011. Detection of Antimicrobial Activity of Oscimum sanctum (Tulsi) and Trigonella foenum graecum (Methi) Against Some Selected Bacterial and Fungsal Strains. Research Journal of Pharmaceutical, Biological and Chemical Sciences 2(4), 809.

Elya, B., Atiek, S., and Farida, 2009. Ekstrak Kulit Batang Manggis Hutan (Garcinia rigida Miq). Jurnal Majalah Ilmu Kefarmasian 6(1), 9-17.

Fajarwati, L., 2008. Isolasi Biflavonoid dari Fraksi Diklorometana pada Kayu Batang Garcinia Picrorrhiza Miq. (Sesoot). Tesis. Surabaya. Institut Teknologi Sepuluh November.

Harwati, S. T., 2009. Dua Senyawa Fenolat Dari Ranting Garcinia Picrorrhiza Miq. Tesis. Surabaya. Institut Teknologi Sepuluh November.

Joseph, G. S., Jayaprakasha, G. K., Selvi, A. T., Jena, B. S., and Sakariah, K. K., 2005. Antiaflatoxigenic and Antioxidant Activities of Garcinia Extracts. International Journal of Food Microbiology 101, 153-160

Lannang, A. M., Komguem, J., Ngninzeko, F. N., Tangmoua, J. G., Lonsti, D., Ajaz, A., Choudhary, M. I., Ranjit, R., Devkota, K. P., and Sondengam, B. L., 2005. Bagangxanthone A and B, Two Xanthones from the Stem Bark of Garcinia polyantha Oliv. Phytochemistry 66, 2351-2355

Novida, and Rizani, 2009. Benzofenon, Biflavonoid Dan Flavonoid DariI Kulit Batang Garcinia picrorrhiza Miq : Isolasi, Penentuan Struktur dan Uji Antioksidan. Tesis. Institut Teknolgi Sebelas November. Surabaya.

Mandy, W., 2013, Methods for Antimicrobial Susceptibility Testing. British Society for Antimicrobial Chemotherapy.

Muharni, Elfita and Perucha, B., 2015, Isolasi Stigmasterol Dari Ekstrak Etil Asetat Biji Garcinia picrorrhiza Miq. Prosiding seminar MIPAnet. Universitas Sriwijaya. Palembang.

Radji, M., Atiek, S., and Nuning, I., 2004. Uji Mutagenisitas dan Anti Kanker Ekstrak Aseton dan n-heksan dari Kulit Batang Garcinia picrorrhiza Miq. Majalah Ilmu Kefarmasian 1(2), 69-78.

Ritthiwigrom, T., Surat, L., and Stephen, G. P., 2013. Chemical Constituents and Biological Activities of Garcinia cowa Roxb. Journal of Science and Technology 7(2), 212-231.

Rishan, S., 2014. Determination of Minimum Inhibitory Concentration of Cycloserine in Multidrug Resistant Mycobacterium tuberculosis Isolates. Jordan Journal of Biological Sciences 7(2), 139 – 145.

Soemiati, A., 2004. Isolasi dan Penentuan Senyawa Kimia serta Uji Aktivitas Biologi dari Buah Tanaman Garcinia dulcis Kurz dan Kulit Batang serta Akar Tanaman Garcinia picrorrhiza Miq. Tesis. Jakarta. Universitas Indonesia.

Soemiati, A., Kosela, S., and Hanafi, 2007. Senyawa triterpenoid dan asam 3-hidroksi-isonikotinat dari ekstrak diklorometana akar Garcinia picrorrhiza Miq. Jurnal Bahan Alam Indonesia 6(2), 73-75.

Wuryanti, and Murnah, 2009. Uji Ekstrak Bawang Bombay Terhadap Anti Bakteri Gram Negatif Pseudomonas aeruginosa dengan Metode Difusi Cakram. Jurnal Sains dan Matematika 17(3), 151-158.

DOI: https://doi.org/10.20961/alchemy.13.2.4346.250-261

Refbacks

  • There are currently no refbacks.