Phosphorus Analysis in Meat using UV-Vis Spectrophotometry with SnCl2 and Hydrazine Sulfate Reduction

Yussi Pratiwi, Rizky Amelia, Aulia Natasya

Abstract

This study aimed to optimize phosphorus analysis in meat using a molybdenum blue reaction involving SnCl2 and hydrazine sulfate as reducing agents to establish the most effective conditions for phosphorus detection. Meat, an essential source of nutrients like phosphorus, plays a vital role in human health, particularly bone and tooth strength. However, overconsumption of phosphorus can lead to health issues such as hyperphosphatemia, making regular monitoring of phosphorus levels in food necessary. The experiment used SnCl2 and hydrazine sulfate under varying acidic conditions to produce a stable blue complex indicative of phosphorus presence. The complex exhibited maximum absorbance in the 689–729 nm wavelength range. Validation of the method showed high linearity with R2 values of 0.9983 and 0.9984 for SnCl2 and hydrazine sulfate, respectively. The molar absorptivity for SnCl2 was 2.093 x 105 L mol-1 cm-1 and 7.92 x 104 L mol-1 cm-1 for hydrazine sulfate. Detection and quantification limits were established, and the %RSD values in each sample ranged from 1.08% to 1.93%, conforming to standard requirements. Upon analysis of meat samples, including duck, chicken, and beef, the phosphorus levels did not meet the Indonesian Nutritionist Association (PERSAGI) standards. This result emphasizes the need for regular phosphorus analysis in meat products to prevent health risks associated with excessive phosphorus intake, such as hyperphosphatemia.

Keywords

Phosphor; SnCl2; Hydrazine Sulphate; UV-Vis Spectrophotometry; Method Validation

Full Text:

PDF

References

[1] A. Emhar, J. M. M. Aji, and T. Agustin, "Analisis Rantai Pasokan (Supply Chain) Daging Sapi di Kabupaten Jember," Berkah Ilmiah Pertanian, vol. 1, no. 3, pp. 53–61, 2014.

Google Scholar

[2] H. C. J. Godfray, P. Aveyard, T. Garnett, J. W. Hall, T. J. Key, J. Lorimer, R. J. Pierrehumbert, P. Scarborough, and S. A. Jebb, "Meat consumption, health, and the environment," Science, vol. 361, no. 6399, 2018,

doi: 10.1126/science.aam5324.

[3] S. Almatsier, Prinsip Dasar Ilmu Gizi. Jakarta: Gramedia Pustaka Utama, 2004,

ISBN: 9789796556861.

[4] K. Vo, L. Bui, G. Oko, E. Okoi, and S. Ayomide, "Validation of UV-Vis Spectrophotometry for Phosphorous Molybdenum Determination in Soils of Vietnam," Chem. Sci. Int. J., vol. 23, no. 2, pp. 1–15, 2018,

doi: 10.9734/csji/2018/42128.

[5] M. S. Calvo and J. Uribarri, "Contributions to Total Phosphorus Intake: All Sources Considered," Semin. Dial., vol. 26, no. 1, pp. 54–61, 2013,

doi: 10.1111/sdi.12042.

[6] B. G. Geisert, G. E. Erickson, T. J. Klopfenstein, C. N. Macken, M. K. Luebbe, and J. C. MacDonald, "Phosphorus requirement and excretion of finishing beef cattle fed different concentrations of phosphorus," J. Anim. Sci., vol. 88, no. 7, pp. 2393-2402, 2010,

doi: 10.2527/jas.2008-1435.

[7] L. A. Calò, V. Savica, and P. A. Davis, "Phosphate Content of Beverages in Addition to Food Phosphate Additives: Real and Insidious Danger for Renal Patients," J. Ren. Nutr., vol. 22, no. 2, pp. 292–293, 2012,

doi: 10.1053/j.jrn.2011.07.008.

[8] E. A. Friedman, "Consequences and management of hyperphosphatemia in patients with renal insufficiency," Kidney Int. Suppl., vol. 67, pp. S1-S7, 2005,

doi: 10.1111/j.1523-1755.2005.09500.x.

[9] J. Serna and C. Bergwitz, "Importance of dietary phosphorus for bone metabolism and healthy aging," Nutrients, vol. 12, no. 10, 3001, 2020,

doi: 10.3390/nu12103001.

[10] C. J. Vorland, E. R. Stremke, R. N. Moorthi, and K. M. Hill Gallant, "Effects of excessive dietary phosphorus intake on bone health," Curr. Osteoporos. Rep., vol. 15, pp. 473-482, 2017,

doi: 10.1007/s11914-017-0398-4.

[11] A. Jastrzȩbska, "Modifications of spectrophotometric methods for total phosphorus determination in meat samples," Chem. Pap., vol. 63, no. 1, pp. 47–54, 2009,

doi: 10.2478/s11696-008-0091-2.

[12] M. Singh, R. Walia, A. Gupta, P. Chawla, H. Kumar, A. Thakur, R. Kaushik, and N. Kumar, "Colorimetric sensing approaches based on silver nanoparticles aggregation for determination of toxic metal ions in a water sample: a review," Int. J. Environ. Anal. Chem., 2021,

doi: 10.1080/03067319.2021.1873315.

[13] Y. Guo, C. Liu, R. Ye, and Q. Duan, "Advances in water quality detection by UV-vis spectroscopy," Appl. Sci., vol. 10, no. 19, 6874, 2020,

doi: 10.3390/app10196874.

[14] Mahadevaiah, M. S. Y. Kumar, M. S. A. Galil et al., “A Simple Spectrophotometric Determination of Phosphate in Sugarcane Juices, Water and Detergent Samples,” J. Chem., vol. 4, 2007,

doi: 10.1155/2007/576560.

[15] N. K. Ibnul and C. P. Tripp, "A simple solution to the problem of selective detection of phosphate and arsenate by the molybdenum blue method," Talanta, vol. 238, 123043, 2022,

doi: 10.1016/j.talanta.2021.123043.

[16] X. Lin, J. Zhang, H. Chen, and L. Han, "Determination of available phosphorus in alkaline soil by molybdenum blue spectrophotometry," IOP Conf. Ser.: Earth Environ. Sci., vol. 781, no. 5, p. 052003, 2021,

doi: 10.1088/1755-1315/781/5/052003.

[17] M. Radojevic and V. Bashkin, Practical Environmental Analysis. Cambridge: The Royal Society of Chemistry, 1999,

ISBN: 9780854046799.

[18] E. A. Nagul, I. D. McKelvie, P. Worsfold, and S. D. Kolev, "The molybdenum blue reaction for the determination of orthophosphate revisited: Opening the black box," Anal. Chim. Acta, vol. 890, pp. 60-82, 2015,

doi: 10.1016/j.aca.2015.07.030.

[19] S. A. Apriliyani, Y. Martono, C. A. Riyanto et al., “Validation of UV-VIS Spectrophotometric Methods for Determination of Insulin Levels from Lesser Yam (Dioscorea esculenta L.),” Jurnal Kimia Sains & Aplikasi, vol. 21, no. 4, pp. 161-165, 2018,

doi: 10.14710/jksa.21.4.161-165.

[20] Pescok, Modern Methods of Chemical Analysis, 2nd ed. New York: John Wiley & Sons, 1976,

ISBN: 9789681809065.

[21] I. Khaldun, Kimia Analisa Instrumen: Buku untuk mahasiswa. Aceh: Syiah Kuala University Press, 2018,

ISBN: 9786025679698.

[22] Harmita, "Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya," Majalah Ilmu Kefarmasian, vol. 1, no. 3, pp. 117-135, 2004,

doi: 10.7454/psr.v1i3.3375.

[23] D. Sistesya and H. Sutanto, "Sifat optis lapisan ZnO: Ag yang dideposisi di atas substrat kaca menggunakan metode chemical solution deposition (Csd) dan aplikasinya pada degradasi zat warna methylene blue," Youngster Phys. J., vol. 2, no. 3, pp. 71-80, 2013.

Google Scholar

[24] D. Hanwar, D. E. Nitoviani, and A. Suhendi, "Validation of Atomic Absorption Spectrometry Method for Contamination Determination of Lead (Pb) and Cadmium (Cd) in Methanol Extract and Product of Curcuma xanthorrhiza Roxb," JKPK (Jurnal Kimia dan Pendidikan Kimia), vol. 2, no. 3, pp. 198-205, 2017,

doi: 10.20961/jkpk.v2i3.11968.

[25] S. Rusmalina, E. R. Wati, K. Khasanah & B. D. Madusari, “Analisis Kuantitatif Cemaran Logam pada Produk Inovasi Sediaan Jamu Serbuk Instan Kunyit Asam Melalui Pembentukan Kompleks Logam-Ditizone,” Benzena Pharmaceutical Scientific Journal, vol. 2, no. 1, 2023,

doi: 10.31941/benzena.v2i01.2383.

[26] T. K. Pulliainen & H. C. Wallin, “Determination of Total Phosphorus in Foods by Colorimetry: Summary of NMKL Collaborative Study,” Journal of AOAC INTERNATIONAL, vol. 79, no. 1, pp. 1408-1410, 2020,

doi: 10.1093/jaoac/79.6.1408.

DOI: https://doi.org/10.20961/jkpk.v8i3.78607

Refbacks

  • There are currently no refbacks.


Bookmark and Share