Determination Stability of 99mTc-Etambutol Radiopharmaceuticals for Tuberculosis Detected

Anna Roseliana, Witarti Witarti, Enny Lestari, Maula Eka Sriyani, Widyastuti Widjaksana


99mTc-ethambutol radiopharmaceutical is a diagnostic kit based on the anti-tuberculosis drug, ethambutol, labeled with radioactive tracer 99mTc.  This radiopharmaceutical is a result of research development in Centre for Radioisotope and Radiopharmaceutical Technology, BATAN. 99mTc-ethambutol radiopharmaceutical in the hospital used as a diagnostic agent for tuberculosis detected, particularly tuberculosis in extra pulmonary organs that is not easily detected by existing  conventional methods. The aim of study to find out how stable ethambutol kit when stored at certain temperature and how stable ethambutol stability after labelled  with 99mTc.  These stability data are required by hospitals which used 99mTc-ethambutol . The stability test performed by measuring the radiochemical purity of  ethambutol kits which were stored at certain time period and labelled with 99mTc using thin layer chromatography (TLC) method. Radiochemical purity tests were also performed on 99mTc-ethambutol at certain time period after its labeling process.  Based on experiment results, it was obtained that ethambutol kit was still stable for up to 9 months when stored at refrigerator temperature 8 oC with percentage of radiochemical purity average > 85% and up to 11 months when stored in deep freezer temperature -80 oC with percentage of radiochemical purity average 90%. While 99mTc-ethambutol was still stable for up to 4 hours after labeling with  percentage of radiochemical purity more than > 85%.


radiopharmaceuticals; ethambutol kit; detection; tuberculosis; stability

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S. S. Das, A. V. Hall, D. W. Wareham, and K. E. Britton, ”Infection Imaging with Radiopharmaceutical in 21st Century,” Brazilian Archives of Biology and Technology, vol. 45 (Spe), pp. 25-37, September. 2002.

S. S. Das, and K. E. Britton, ”Bacterial Infection Imaging,” World. J. Med, vol. 2, no. 3, pp. 173-179, July. 2003.

J. Verma, A. K. Singh, A. Bhatnagar, S. Sen, and M. Bose, “Radiolabeling of ethambutol with technetium-99m and its evaluation for detection of Tuberculosis,” World Journal of Nuclear Medicine, vol. 4, no. 1, pp. 35-46, January. 2005.

N. K. Oekar, Kustiwa, dan E. Isabela, “Pengembangan senyawa bertanda99mTc-Etambutol untuk diagnosis Tuberkulosis ; 1. Penandaan etambutol dengan radio-nuklida teknesium-99m,” Prosiding Seminar Sains dan Teknologi Nuklir, Puslitbang Teknik Nuklir, BATAN, Bandung, 2005, pp. 137-145.

N. K. Oekar, Kustiwa, dan E. Susilawati, “Pengembangan senyawa bertanda 99mTc-Etambutol untuk diagnosis Tuberkulosis ; 1. Karakterisasi Fisiko-Kimia dan Mikrobiologis,” Jurnal Sains dan Teknologi Nuklir Indonesia, vol. 8, no. 1, pp. 17-28, Februari . 2007.

N. K. Oekar, “Kit-Diagnostik Berbasis Teknik Nuklir untuk Penatalaksanaan Penyakit Tuberkulosis (TBC),” Majalah Kedokteran Indonesia, vol. 58, no.10, Oktober. 2008.

H. Wangsaatmadja dan N. K. Oekar, “Profil Farmako Kinetika Radiofarmaka Etambutol Bertanda Teknesium -99m sebagai Sediaan Sidik Tuberkulosis,” Jurnal Farmasi Indonesia, vol. 5, no. 4, pp. 190-196, Juli. 2011.

Departemen Kesehatan RI, Farmakope Indonesia, Ed IV, Jakarta, 1995.

A. H. S. Kartamihardja, Y. Kurniawati, and R. Gunawan, “Diagnostic value of 99mTc-ethambutol scintigraphy in tuberculosis: compared to microbiological and histopathological tests”. World Journal of Nuclear Medicine, Vol 32,no 1, pp 60–68, Januari 2018


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