Efek Pemberian Ekstrak Etanol Daun Tephrosia vogelii Hook. f. terhadap Histologi Hati Tikus Putih Jantan Rattus norvegicus

Dewi Ratnasari, Valentina Adimurti Kusumaningtyas, Arie Hardian

Abstract

Tephrosia vogelii Hook.f. tanaman yang secara tradisional digunakan untuk pengobatan demam, diare, penyakit kulit, malaria, dan meningitis, serta sebagai pestisida alami. Penelitian ini bertujuan untuk mengetahui efek toksik pemberian berulang ekstrak etanol daun T. vogelii terhadap berat dan histologi hati serta menentukan konsentrasi ekstrak etanol T. vogelii yang  aman terhadap liver tikus putih (Rattus norvegicus) jantan. Objek dalam penelitian ini daun T. vogelii. Metode penelitian menggunakan desain Rancangan Acak Lengkap yang terdiri dari 5 perlakuan dan 6 kali pengulangan. Penelitian menggunakan parasetamol 50 mg/kg BB sebagai kontrol positif, air mineral sebagai kontrol negatif dan larutan uji ekstrak etanol daun T. vogelii dengan dosis 50mg/kg BB, 100 mg/kg BB dan 200 mg/kg BB dilakukan selama 28 hari. Parameter yang diamati adalah berat hati, inflamasi sel, dan steatosis. Hasil penelitian menunjukkan bahwa ekstrak etanol daun T. vogelii tidak berpengaruh nyata terhadap indeks organ (P>0,05), tidak menimbulkan inflamasi dan steatosis. Esktrak etanol daun T. vogelii dengan dosis 50, 100, dan 200 mg/kg BB tidak berpengaruh terhadap indeks organ, dan aman terhadap hati tikus putih jantan.

Effect of Ethanolic Extract of Tephrosia vogelii Hook. f. Leaves on the Liver Histology of Male White Rats of Rattus norvegicus. Tephrosia vogelii Hook.f. is a plant traditionally used to treat fever, diarrhea, skin diseases, malaria, meningitis, and as a natural pesticide. This study aims to determine the toxic effect of repeated administration of T. vogelii leaves ethanol extract on liver weight and histology, and to establish the safe concentration of T. vogelii leaves ethanol extract for the liver of male white rats (Rattus norvegicus). The research method focuses on the leaf part. The study used a Completely Randomized Design with five treatments and six replications. Treatments included 50 mg/kg BW of paracetamol as a positive control, mineral water as a negative control, and test solutions of ethanol extract of T. vogelii leaves at doses of 50 mg/Kg BW, 100 mg/kg BW, and 200 mg/kg BW, administered over 28 days. The observed parameters were liver weight, cell inflammation, and steatosis. The research results show that the ethanol extract of T. vogelii has no significant effect on the organ index (P>0.05) and does not cause inflammation or steatosis. The ethanol extract of T. vogelii at doses of 50, 100, and 200 mg/kg BW does not affect the organ index and is safe for the liver of male white rats.

Keywords

organ indeks; inflammation; steatosis; Tephrosia vogelii; white rats

Full Text:

PDF

References

Arora, S.K., Verma, P.R., Itankar, P.R., Prasad, S.K., and Nakhate, K.T., 2021. Evaluation of Pancreatic Regeneration Activity of Tephrosia purpurea Leaves in Rats with Streptozotocin-Induced Diabetes. Journal of Traditional and Complementary Medicine, 11, 435–445. https://doi.org/10.1016/j.jtcme.2021.03.001.

Aryaeian, N., Alipour, R., Jafari Karegar, S., Soleimani, M., Hosseini, A., and Hekmatdoost, A., 2024. Saffron Effects on Liver Enzymes, Antioxidant Capacity, Insulin, Inflammation and Genes Expression of Lipolysis and Lipogenesis in a Rat Model of Non-Alcoholic Fatty Liver Disease. Clinical Nutrition Open Science, 53, 95–107. https://doi.org/10.1016/j.nutos.2023.12.004.

Bawa, I., Uti, D.E., Itodo, M.O., Umoru, G.U., Zakari, S., and Obeten, U.N., 2022. Effect of Solvent Extracts of Tephrosia Vogelii Leaves and Stem on Lipid Profile of Poloxamer 407-Induced Hyperlipidemic Rats. Ibnosina Journal of Medicine and Biomedical Sciences, 14, 135–144. https://doi.org/10.1055/s-0042-1760223.

Chali, Y.N., and Sime, T.S., 2019. Phytochemical Investigation and Characterization of the Chemical Constituents from Root Extracts of Tephrosia vogeli. International Journal of Novel Research in Engineering and Science, 6, 1–14.

Fitmawati, F., Titrawani, T., and Safitri, W., 2019. Struktur Histologi Hati Tikus Putih (Rattus norvegicus Berkenhout 1769) Dengan Pemberian Ramuan Tradisional Masyarakat Melayu Lingga, Kepulauan Riau. EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi, 3, 11–19. https://doi.org/10.33019/ekotonia.v3i1.753.

Goh, G.B.-B., Leow, W.Q., Liang, S., Wan, W.K., Lim, T.K.H., Tan, C.K., and Chang, P.E., 2019. Quantification of Hepatic Steatosis in Chronic Liver Disease Using Novel Automated Method of Second Harmonic Generation and Two-Photon Excited Fluorescence. Scientific Reports, 9, 2975. https://doi.org/10.1038/s41598-019-39783-1.

Hasan, A.E.Z., Setiyono, A., and Afrian, M., 2022. Hepatoprotective Activity of Propolis Trigona Spp., Hibiscus Sabdariffa, and Myrmeleon Sp. in Rats Induced by Paracetamol. Current Biochemistry, 9, 38–50. https://doi.org/10.29244/cb.9.1.4.

Hidayat, M., Prahastuti, S., Rakasiwi, A.S., Prisilia, S., and Hasan, K., 2022. Sub-Chronic Toxicity Study of Green Peas Protein Hydrolysate in Rats. Toxicology Reports, 9, 735–742. https://doi.org/10.1016/j.toxrep.2022.03.020.

Husna, F., Suyatna, F.D., Arozal, W., and Purwaningsih, E.H., 2019. Model Hewan Coba pada Penelitian Diabetes. Pharmaceutical Sciences and Research, 6, 131–141. https://doi.org/10.7454/psr.v6i3.4531.

Isdadiyanto, S., and Tana, S., 2019. Struktur Histologi Hepar Tikus Wistar (Rattus norvegicus) Jantan Setelah Pemberian Teh Kombucha Konsentrasi 75% dengan Waktu Fermentasi yang Berbeda. Bioma: Berkala Ilmiah Biologi, 21, 165–172. https://doi.org/10.14710/bioma.21.2.165-172.

Islam, M.T., Quispe, C., Islam, Md.A., Ali, E.S., Saha, S., Asha, U.H., Mondal, M., Razis, A.F.A., Sunusi, U., Kamal, R.M., Kumar, M., and Sharifi-Rad, J., 2021. Effects of Nerol on Paracetamol-Induced Liver Damage in Wistar Albino Rats. Biomedicine & Pharmacotherapy, 140, 111732. https://doi.org/10.1016/j.biopha.2021.111732.

Kansatwad, P.B., Ranvir, G.D., Ballurkar, B. V, Rajurkar, S.R., Jadhav, N.D., and Ghadge, P.M., 2020. Evaluation of Hepatoprotective and Antioxidant Activity of Tephrosia purpurea L. against Carbon Tetrachloride Intoxicated Wistar Rats. The Pharma Innovation Journal, 9, 290–296.

Kerebba, N., Oyedeji, A.O., Byamukama, R., Kuria, S.K., and Oyedeji, O.O., 2019. Pesticidal Activity of Tithonia diversifolia (Hemsl.) A. Gray and Tephrosia vogelii (Hook f.); Phytochemical Isolation and Characterization: A Review. South African Journal of Botany, 121, 366–376. https://doi.org/10.1016/j.sajb.2018.11.024.

Kusumaningtyas, V.A., Hardianti, N.S., Melina, M., Juliawaty, L.D., and Syah, Y.M., 2020. A Cytotoxic Flavanone from The Pod Peels of Theprosia Vogelii Hook.f. Jurnal Kimia Valensi, 6, 140–145. https://doi.org/10.15408/jkv.v6i2.17551.

Kusumaningtyas, V.A., Supratman, U., Aryanti, P.T.P., Melina, M., and Juliawaty, L.D., 2024. Tephrosin and Obovatachalcone with Antibacterial Activity from Tephrosia vogelii Hook.f. Natural Product Research, 1–6. https://doi.org/10.1080/14786419.2024.2405878.

Lazic, S.E., Semenova, E., and Williams, D.P., 2020. Determining Organ Weight Toxicity with Bayesian Causal Models: Improving on the Analysis of Relative Organ Weights. Scientific Reports, 10, 6625. https://doi.org/10.1038/s41598-020-63465-y.

Maronpot, R.R., n.d. Liver - Inflammation. U.S. Department of Health and Human Service.

Ningrum, E.W.C., Isdadiyanto, S., and Mardiati, S.M., 2020. Histopatologi Pankreas Tikus Putih (Rattus norvegicus L.) yang Diberi Pakan Tinggi Lemak dan Paparan Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss). Buletin Anatomi dan Fisiologi, 5, 129–137. https://doi.org/10.14710/baf.5.2.2020.129-137.

Orchard, G.E., 2013. Pigments and Minerals, in: Bancroft’s Theory and Practice of Histological Techniques. Elsevier, pp. 239–270. https://doi.org/10.1016/B978-0-7020-4226-3.00013-5.

Pedoman Uji Toksisitas Praklinik secara In Vivo, 2022. Peraturan Badan Pengawas Obat dan Makanan No. 10 Tahun 2022. Badan Pengawan Obat dan Makanan. Jakarta.

Safira, M.N., Apridamayanti, P., Kurniawan, H., Fajriaty, I., Nugraha, F., Nurbaeti, S.N., and Pratiwi, L., 2023. Pengaruh Pemberian Kombinasi Ekstrak Kulit Pisang dan Kulit Nanas terhadap Indeks Organ Tikus Wistar. Journal Syifa Sciences and Clinical Research, 4, 227–236. https://doi.org/10.37311/jsscr.v4i1.13910.

Sameemabegum, S., Thilagavathi, R., Kumarnallasivan, P., and Sivakumar, T., 2024. Hepatoprotective Effect of Ethanolic Extract of Whole Plant of Tephrosia barberi J. R. Drumm. in Paracetamol Induced Hepatotoxicity in Rats. Journal of Natural Remedies, 24, 1525–1535. https://doi.org/10.18311/jnr/2024/36162.

Samuel, V.J., Mahesh, A.R., and Murugan, V., 2019. Phytochemical and Pharmacological Aspects of Tephrosia Genus: A Brief Review. Journal of Applied Pharmaceutical Science, 9, 117–125. https://doi.org/10.7324/JAPS.2019.90317.

Shah, R., Parmar, S., Bhatt, P., and Chanda, S., 2011. Evaluation of Hepatoprotective Activity of Ethyl Acetate Fraction of Tephrosia Purpurea, Pharmacologyonline.

Sharma, T., Pandey, B., Shrestha, B.K., Koju, G.M., Thusa, R., and Karki, N., 2020. Phytochemical Screening of Medicinal Plants and Study of the Effect of Phytoconstituents in Seed Germination. Tribhuvan University Journal, 35, 1–11. https://doi.org/10.3126/tuj.v35i2.36183.

Sidabutar, D.M., Kairupan, C.F., and Durry, M., 2016. Pengaruh Pemberian Ekstrak Daun Cengkeh (Syzygium aromaticum) terhadap Gambaran Histopatologik Hati Tikus Wistar yang Diberikan Parasetamol Dosis Toksik. Jurnal e-Biomedik, 4. https://doi.org/10.35790/ebm.4.1.2016.12224.

Sijid, S.A., Muthiadin, C., Zulkarnain, Z., and Hidayat, Ar.S., 2020. Pengaruh Pemberian Tuak terhadap Gambaran Histopatologi Hati Mencit (Mus musculus) ICR Jantan. Jurnal Pendidikan Matematika dan IPA, 11, 193–205. https://doi.org/10.26418/jpmipa.v11i2.36623.

Sudisma, I.G.N., Soma, I.G., Sudira, I.W., and Rastiti, N.M., 2023. Respon Klinis dan Fisiologis Tikus Putih (Rattus norvegicus) yang Diberikan Ekstrak Bunga Kecubung (Datura metel L.) Sebagai Anestesi. Jurnal Sain Veteriner, 41, 323–335. https://doi.org/10.22146/jsv.74314.

Teja, P.T.H.S., Arjana, A.A.G., Setiasih, N.L.E., and Merdana, I.M., 2021. The Impact Oral Administration of Minyak Rajas on Liver Histopatology and Aminotranspherase Activities in Male Kampong Chicken. Indonesia Medicus Veterinus, 10, 233–244. https://doi.org/10.19087/imv.2021.10.2.233.

Wowor, M., Loho, L., and Lintong, P.M., 2018. Gambaran Histopatologik Hati Tikus Wistar yang Diberikan Sari Buah Naga Merah (Hylocereus polyrhisus) dan Parasetamol. Jurnal e-Biomedik, 6, 16–20. https://doi.org/10.35790/ebm.6.1.2018.18680.

DOI: https://doi.org/10.20961/alchemy.21.2.97702.326-336

Refbacks

  • There are currently no refbacks.