Structure, Amylograph, and Digestibility Properties of Adlay (Coix lacryma-jobi L) Seed Starch

Siti masithah Fiqtinovri, Didi Adriansyah, Risnafaty .


Jelai (Coix lacryma jobi L.) that is cultivated in Bulungan Regency has thin and soft skin, yellowish brown in color and slightly shiny surface. The high content of starch and other components in barley increases the potential of Jelai as a food that has functional properties. This study aims to examine the relationship between the structural characteristics, amylograph properties, and digestion of Jelai natural starch (PAJ) and compare it with commercial cassava starch (PS) and corn starch (PJ). The research is started with the isolation process of jelai starch and then structural observations were carried out by SEM test, amylograph properties through RVA test, and digestibility including rapidly digestible starch (RDS), slowly digestible starch (SDS), and resistant starch (RS). PAJ has a low amylose content and a polygonal morphology with a clearly visible central pore / channel. The crystallinity of PAJ is slightly high with A-type crystalline, the same as PJ and PS. The starch structure in PAJ contributed to a slightly lower gelatinization temperature compared to PJ and the lowest setback viscosity compared to PJ and PS which indicated that PAJ was more resistant to retrogradation, as well as the highest RS levels and the lowest RDS compared to PJ and PS. 


Jelai native starch; cassava starch; corn starch; starch structure; amylograph properties; starch digestibility


Bai, X., Dong, Z., Wu, X., Tong, J., & Zhou, J. (2013). Changes in crystalline structure of microspheres of corn starch and amylose under isothermal and temperature cycling treatments. Industrial Crops and Products, 51, 220–223.

Bamforth, C. W. (2005). Food , Fermentation and Micro-organisms. USA: Blackwell Publishing Company.

Chen, J., Chen, Y., Ge, H., Wu, C., Pang, J., & Miao, S. (2019). Multi-scale structure , pasting and digestibility of adlay (Coixlachryma-jobi L .) seed starch. Food Hydrocolloids, 89(November 2018), 885–891.

Chi, C., Li, X., Zhang, Y., Chen, L., Li, L., & Wang, Z. (2017). Food & Function. Food & Function, (January).

Copeland, L., Blazek, J., Salman, H., & Tang, M. C. (2009). Food Hydrocolloids Form and functionality of starch. Food Hydrocolloids, 23(6), 1527–1534.

Corke, H., Kong, H., & Kong, H. (2016). Coix : Overview, 1–6.

Dona, A. C., Pages, G., Gilbert, R. G., & Kuchel, P. W. (2010). Digestion of starch: In vivo and in vitro kinetic models used to characterise oligosaccharide or glucose release. Carbohydrate Polymers, 80(3), 599–617.

Englyst, H. N., Kingman, S. M., & Cummings, J. H. (1992). Classification and measurement of nutritionally important starch fractions. In European Journal of Clinical Nutrition.

Englyst, K., Goux, A., Meynier, A., Quigley, M., Englyst, H., Brack, O., & Vinoy, S. (2018). Inter-laboratory validation of the starch digestibility method for determination of rapidly digestible and slowly digestible starch. Food Chemistry, 245(August 2017), 1183–1189.

Fiqtinovri, S. M. (2020). Karakteristik Kimia dan Amilografi Mocaf (Modified Cassava Flour) Singkong Gajah (Manihot Utilissima), 6(April), 49–56.

Fiqtinovri, S. M., Benita, A. M., Marseno, D. W., & Pranoto, Y. (2020). Sifat Fisik, Amilograf, dan Morfologi Pati Biji Lai (Durio kutejensis) Asetilasi Menggunakan Asetat Anhidrat. AgriTECH, 40(1), 74.

Gidley, M. J., Hanashiro, I., Hani, N. M., Hill, S. E., Huber, A., Jane, J. L., Liu Q., Morris G.A., Rolland-Sabate A., Striegel A. M., Gilbert, R. G. (2010). Reliable measurements of the size distributions of starch molecules in solution: Current dilemmas and recommendations. Carbohydrate Polymers, 79(2), 255–261.

Goñi, I., Garcia-Alonso, A., & Saura-Calixto, F. (1997). A starch hydrolysis procedure to estimate glycemic index. Nutrition Research.

Htoon, A., Shrestha, A. K., Flanagan, B. M., Lopez-Rubio, A., Bird, A. R., Gilbert, E. P., & Gidley, M. J. (2009). Effects of processing high amylose maize starches under controlled conditions on structural organisation and amylase digestibility. Carbohydrate Polymers, 75(2), 236–245.

Kesarwani, A., Chiang, P. Y., & Chen, S. S. (2016). Rapid Visco Analyzer Measurements of japonica Rice Cultivars to Study Interrelationship between Pasting Properties and Farming System. International Journal of Agronomy, 2016(February).

Kim, M. J., Choi, S. J., Shin, S. I., Sohn, M. R., Lee, C. J., Kim, Y., Cho W. I., Moon, T. W. (2008). Resistant glutarate starch from adlay: Preparation and properties. Carbohydrate Polymers, 74(4), 787–796.

Kittipongpatana, O. S., Sirithunyalug, J., & Laenger, R. (2006). Preparation and physicochemical properties of sodium carboxymethyl mungbean starches. Carbohydrate Polymers, 63(1), 105–112.

Li, C., Chen, G., Ran, C., Liu, L., Wang, S., & Xu, Y. (2019). Adlay starch-gluten composite gel : E ff ects of adlay starch on rheological and structural properties of gluten gel to molecular and physico-chemical characteristics. Food Chemistry, 289(September 2018), 121–129.

Li, J., & Corke, H. (1999). Physicochemical properties of normal and waxy job’s tears (Coix lachryma-jobi L.) starch. Cereal Chemistry, 76(3), 413–416.

Liu, X., Wu, J., Xu, J., Mao, D., Yang, Y., & Wang, Z. (2016). The impact of heat-moisture treatment on the molecular structure and physicochemical properties of Coix seed starches, 1–13.

Liu, X., Zhang, B., Xu, J., Mao, D., Yang, Y., & Wang, Z. (2016). Rapid determination of the crude starch content of Coix seed and comparing the pasting and textural properties of the starches, 1–8.

Man, J., Cai, J., Cai, C., Xu, B., Huai, H., & Wei, C. (2012). Comparison of physicochemical properties of starches from seed and rhizome of lotus. Carbohydrate Polymers, 88(2), 676–683.

Miao, M., Jiang, B., Cui, S. W., Zhang, T., & Jin, Z. (2015). Slowly Digestible Starch—A Review. Critical Reviews in Food Science and Nutrition, 55(12), 1642–1657.

Piroski, C. S., Bedin, A. C., Lacerda, L. G., Nogueira, A., & Demiate, I. M. (2021). In Vitro Digestibility of Starch from Ready-to-Eat Cassava and Corn Flours. Brazilian Archives of Biology and Technology, 64, 1–14.

Shafie, B., Cheng, S. C., Lee, H. H., & Yiu, P. H. (2016). Characterization and classification of whole-grain rice based on rapid visco analyzer (RVA) pasting profile. International Food Research Journal, 23(5), 2138–2143.


Syahputri, D. A., & Wardani, A. K. (2015). PENGARUH FERMENTASI JALI ( Coix lacryma jobi-L) PADA PROSES PEMBUATAN TEPUNG TERHADAP KARAKTERISTIK FISIK DAN KIMIA COOKIES DAN ROTI TAWAR Effects of Jali ( Coix lacryma jobi-L ) Fermentation in Flour Produstion on Physical and Chemical Characteristics o, 3(3), 984–995.

Topping, D. L., Bajka, B. H., Bird, A. R., Clarke, J. M., Cobiac, L., Conlon, M. A., Morell M. K., Toden, S. (2008). Resistant starches as a vehicle for delivering health benefits to the human large bowel. Microbial Ecology in Health and Disease, 20(2), 103–108.

Topping, D. L., & Clifton, P. M. (2001). Short-chain fatty acids and human colonic function: Roles of resistant starch and nonstarch polysaccharides. Physiological Reviews, 81(3), 1031–1064.

Ulfa, G. M., Putri, W. D. R., Fibrianto, K., Prihatiningtyas, R., & Widjanarko, S. B. (2020). The influence of temperature in swelling power, solubility, and water binding capacity of pregelatinised sweet potato starch. IOP Conference Series: Earth and Environmental Science, 475(1).

Vesterinen, E., Myllärinen, P., Forssell, P., Söderling, E., & Autio, K. (2002). Structural properties in relation to oral enzymatic digestibility of starch gels based on pure starch components and high amylose content. Food Hydrocolloids, 16(2), 161–167.

Wang, L., Chen, C., Su, A., Zhang, Y., Yuan, J., & Ju, X. (2016). Structural characterization of phenolic compounds and antioxidant activity of the phenolic-rich fraction from defatted adlay ( Coix lachryma-jobi L . var . ma-yuen Stapf ) seed meal. FOOD CHEMISTRY, 196, 509–517.

Wu, T. T., Charles, A. L., & Huang, T. C. (2007). Determination of the contents of the main biochemical compounds of Adlay (Coxi lachrymal-jobi). Food Chemistry, 104(4), 1509–1515.

Yu, S., Ma, Y., Menager, L., & Sun, D. W. (2012). Physicochemical Properties of Starch and Flour from Different Rice Cultivars. Food and Bioprocess Technology, 5(2), 626–637.

Zhu, F. (2017). Coix: chemical composition and health effects. Trends in Food Science & Technology.


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