Uji Toleransi Logam Berat Bakteri Hidrokarbonoklastik dan Uji Kemampuan Micrococcus sp. LII61 dalam Menurunkan Kromium (Cr VI), Tembaga (Cu II), Seng (Zn II)

Achmad Zainal Abidin, Elga Renjana, Fatimah Fatimah, Ni’matuzahroh Ni’matuzahroh

Abstract


This research aimed to know: (1) tolerance level of hydrocarbon-degrading bacteria collected from Biology Laboratory of Airlangga University i.e. Acinetobacter sp P2(1) Bacillus subtilis 3KP, Micrococcus sp. LII61, Pseudomonas putida T1(8), at various concentration of heavy metal chromium (Cr6+), copper (Cu2+), zinc (Zn2+), and combination of that three metals; (2) growing ability of selected bacteria in lowering metals concentration; and (3) reduction percentage of metal concentration by selected bacteria. Tolerance test was performed using streak plate method on two types of solid mediums, i.e. Mineral Salt Medium (MSM) agar and Mueller-Hinton Agar (MHA) with a stratified concentration and 72 hours of incubation time. Bacterial growth and metal concentration reduction test were carried out in liquid medium containing single metal or combined metals. Viability of bacteria was calculated using Total Plate Count (TPC) method. Logarithmic of TPC were analyzed statistically using Brown-Forsythe test followed by Games-Howell. The results showed: (1) All bacteria was resistant to either single heavy metal or combined metals on both medium. However, Micrococcus sp. LII61, had the highest tolerance compared to the other three bacteria, which was able to grow at Zn+2 1000 ppm in both medium. Micrococcus sp. LII61, was the selected bacteria to be tested for its ability to reduce heavy metal by single heavy metal and combined metals for 3 and 7 days of incubation time. (2) Ability levels of Micrococcus sp. LII61, to grow in some heavy metals was Zn2+ > Cu2+ > Cr6+ consecutively. (3) Ability levels of Micrococcus sp. LII61, in reducing heavy metals was Cu2+ > Zn2+ > Cr6+ consecutively. Percentage decrease of Cu2+ in single metal was 66.15%, while in combination metals was 54.25% at 3 days incubation. Percentage decrease of Cu2+ after seven day incubation in single metals and combination metals were 90.13% and 74.72% respectively.

Keywords


Hydrocarbon-degrading bacteria, heavy metals, Micrococcus sp. LII61, tolerance

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References


Acikel, Y.S. (2011). Use of biosurfactants in the removal of heavy metal ions from soils. Environmental Pollution 20: 182-223.

Alam, M.Z, Ahmad, S, & Malik, A. (2011). Prevalence of heavy metal resistance in bacteria isolated from tannery effluents and affected soil. Environmental Monitoring and Assessment, 178: 281:291.

Ali, H. & Khan, E. (2018). Trophic transfer, bioaccumulation, and biomagnification of non-essential hazardous heavy metals and metalloids in food chain/webs-concepst and implications for wildlife and human health. Human and Ecological Risk Asssessment: An International Journal, 24(8): 1-24.

Anwar, M., Ali, S., & Al-Taee, A. (2017). Evaluation of heavy emtals resistant Micrococcus sp. isolated from Rivers in Basra, Iraq. Journal of Bioremediation and Biodegradation, 8 (1): 2-4.

Benmalek, Y. & Fardeau, M.L. (2016). Isolation and characterization of metal-resistant bacterial strain from wastewater and evaluation of its capacity in metal-ions removal using living and dry bacterial cells. Internation Journal Environmental Science and Technology, 13: 2153-2162.

Binupriya, A.R, Sathiskumar, M, Swaminathan, K, Jeong E.S., Yun, S.E., & Pattabi, S. (2006). Biosorption of metal ions from aqueous solution and electroplating industry wastewater by 7Aspergillus japonicus: Phytotoxicity studies. Bulletin Environmental Contamination and Toxicoly, 77: 219-27

Cabral, L, Giovanella, P, Gianello, C, Bento, F.M, Andreazza, R, Camargo, F.A.O. (2012). Isolation and characterization of bacteria from mercury contaminated sites in Rio Grande do Sul, Brazil, and assessment of methylmercury removal capability of a Pseudomonas putida V1 strain. Biodegradation, 24: 319-331.

Cabrero, A, Fernandez,S, Mirada, F, & Garcia, J. (1998). Effects of copper and zinc on the activated sludge bacteria growth kinetics. Water Research, 32(5): 1355-1362.

Chen, X, Shi, J, Chen, Y, Xu, X, Xu, S, & Wang, Y. (2006). Tolerance and biosorption of copper and zinc by Pseudomonas putida CZ1 isolated from metal polluted soil. Canadian Journal of Microbiology , 52 (4): 308-316.

Chen, X.C, Wang, Y.P, Lin, Q, Shi, J.Y, Wu, W.X, & Chen, Y.X. (2005). Biosorption of copper (II) and zinc (II) from aqueous solution by Pseudomonas putida CZ1. Colloids and Surfaces B, 46: 101-107.

Chunxi, K, Pingxiao, W, yuewu, L, Bo, R, Nengwu, Z, & Zhi, D. (2014). Estimates of heavymetal tolerance and chromium (VI) reducing ability of Pseudomonas aeruginosa CCTCC AB93066: chromium (VI) toxicity and environmental parameters optimization. World Journal Microbiology and Biotechnology, 30: 2733-2746.

Cristani, M, Naccari, C, Nostro, A, Pizzimenti, A, Trombetta, D, & Pizzimenti, F. (2012). Possible use of Serratia marcescens in toxic metal biosorption. Environmental Science and Pollution Research, 19(1): 161-168.

Gupta, P. & Diwan, B. (2017). Bacterial exopolysaccharide mediated heavy metal removal: A review on biosynthesis, mechanism and remediation strategies. Biotechnology Reports, 13: 58-71.

Hassen, A, Saidi, N, Cherif, M, & Boudabus, A. (1998). Resistance of environmental bacteria to heavy metal. Bioresource Technology, 64 (1): 7-15.

Huang, F, &g, Z, Guo, C, Lu, G, Gu, R,R, Liu, H, & Zhang, H. (2013). Biosorption of Cd (II) by live and dead cells of Bacillus cereus RC-1 isolated from cadmium-contaminated soil. Colloids and Surfaces B: Biointerfaces, 107: 11-18.

Irawati, W, Parhusip, A.J.N, & Sopiah, R.N. (2015). Heavy metals biosorption. Microbiology Indonesia, 9(4): 163-170.

Jiang, W, Saxena, A, Song, b, Ward, B.B, Beveridge T.J, & Myneni S.C.B. (2004). Elucidation of functional groups on Gram-positif and Gram-negative bacterial surfaces using infrared spectroscopy. Langmuir, 20: 11433-11442.

Kilic, N.K.. & Donmez, G. (2008). Environmental conditions affecting exopolysaccharide production by Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp. Journal of Hazardous Materials, 154: 1019-1024.

Kumar, R, Nongkhlaw, M, Acharya, C, & Joshi, S.R. (2013). Growth media composition and heavy metal tolerance behaviour of bacteria characterized from the sub-serface soil of uranium rich ore bearing site of Domiasiat in Meghalaya. Indian Journal of Biotechnology, 12: 115-119.

Li, H, Lin, Y, Guan, W, Chang, J, Xu, Lin, Guo, J, & Wei, G. (2010). Biosorption of Zn (II) bu live and dead cells of Steptomyces ciscaucasicus strain CCNWHX 72-14. Journal of hazardous materials, 179: 151-159.

Marzan, L.W, Hossain, M., Mina, S.A, Akter, Y, & Chodhury, A.M.M.A. Isolation and biochemical characterization of heavy-metal resistant bacteria from tannery effluent in Chittagong city, Bangladesh: Bioremediation.

Mohammed, J.N, Aliyu, M.M, Kasim, Z.J., & Gana, M. (2017). Heavy metal tolerance of bacteria isolated from mechanic workshops. Nigerian Journal of Microbiology, 31(1): 3867-3872.

Nugroho, A. (2006). Bioremediasi hidrokarbon minyak bumi. Jakarta, Indonesia: Graha Ilmu.

Oves, M, Saghir Khan, M, & Zaidi, A. (2013). Biosorption of heavy metals by Bacillus thuringiensis strain OSM29 originating from industrial effluent contaminated north Indian soil. Saudi Journal of Biological Sciences, 20: 121-129.

Park, D, Yun, Y.S, & Park, J.M. (2005) Use of dead fungal biomass for the detoxification of hexavalent chromium: screening and kinetics. Process Biochemical, 40: 2559-65.

Pruthi, V. & Comeotra, S.S. (1997) Rapid identification of biosurfactant producing bacterial strain using a cell surface hidrophobicity techniques. Biotechnology Techniques, 11: 671-674.

Puyen, Z.M, Villagrasa, E, Maldonado, J, Diestra, E, Esteve, I, & Sole, A. (2012). Biosorption of lead and copper by heavy-metal tolerant Micrococcus luteus DE2008. Biosource Technology, 126: 233-237.

Rojo, F. (2009). Degradation of alkanes by bacteria. Environmental Microbiology, 11(10): 2477-2490.

Sengor, S.S, Gikas, P, Moberly, J.G, Peyton, B.M, & Ginn, T.R. (2012). Comparison of single and joint effects of Zn and Cu in continous flow and batch reactors. Journal of Chemical Technology and Biotechnology, 87(3): 374-380.

Silva, R.M.P, Rodriguez, A.A, Oca, J.M.G.M.D, & Moreno, D.C. (2009) Biosorption of chromium, copper, manganese and zinc by Pseudomoas aeruginosa AT18 isolated from a site contaminated with petroleum. Bioresource Technology, 100: 1533-1538.

Tan, T, & Cheng, P. (2003). Biosorption of metal ions with Penicillium chrysogenum. Applied of Biochemistry and Biotechnology, 104(2):119-128.

Tunali, S, Cabuk, A, & Akar, T. (2006). Removal of lead and copper ions from aqueous solutions by bacterial strain isolated from soil. Chemical Engineering JOurnal, 115(3): 203-211.

Uslu, G & Tanyol, M. (2006). Equilibrium and thermodynamic parameters of single and binary mixture biosorption of lead (II) and copper (II) ions onto Pseudomonas putida: effect of temperature. Journal of Hazardous Material, 135: 87-93.

Vijayaraghavan, K. & Yun, Yeoung-Sang. (2008). Bacterial biosorbents and biosorption. Biotechnology advances, 26: 266-291.

Wang, Q, Dai, J, Yu, Y, Shen, T, Liu, J, & Wang, R. (2010). Efficiencies of different microbial parameters as indicators to asses slight metal pollution in a farm near a gold mining area. Environmental Monitoring and Assessment, 161: 495-508.

Wani, P.A. & Ayoola, O.H. (2015). Bioreduction of Cr (IV) by heavy metal resistant Pseudomonas species. Journal of Environmental Science and Technology, 8(3): 122-130.

Yilmaz, E.I. (2003). Metal tolerance and biosorption capacity of Bacillus circulans strain EB1. Research in microbiology, 154: 409-415.




DOI: https://doi.org/10.20961/bioedukasi-uns.v12i1.27414

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