VALIDASI METODE ANALISIS SURFAKTAN ANIONIK NATRIUM DODESIL BENZENA SULFONAT (SDBS) DALAM IKAN LELE SECARA SPEKTROFOTOMETRI UV-VIS MENGGUNAKAN ACRIDINE ORANGE

Monica Cahyaning Ratri

Abstract

ABSTRAK

 

Pengembangan metode analisis surfaktan anionik natrium dodesil benzena sulfonat (SDBS) secara spektrofotometri UV-Vis menggunakan acridine orange (AO) dalam ikan lele telah dilakukan. Penelitian ini bertujuan untuk menentukan kondisi optimum analisis dan parameter validasi metode analisis dodesil benzena sulfonat (SDBS) dalam tubuh ikan lele.  Penelitian ini dibagi dua tahap, yaitu isolasi natrium dodesil benzena sulfonat (SDBS) dalam tubuh ikan lele dengan ekstraksi Soxhlet dan analisis natrium dodesil benzena sulfonat (SDBS). Analisis natrium dodesil benzena sulfonat (SDBS) didasarkan pada pembentukan pasangan ion antara dodesil benzena sulfonat (DBS) dan acridine orange (AO).

            Hasil penelitian menunjukkan bahwa panjang gelombang maksimum pasangan ion dodesil benzena sulfonat (DBS) dan acridine orange (AO) adalah 499 nm, acridine orange (AO) larut dalam etanol, perbandingan mol dodesil benzena sulfonat (DBS) dan acridine orange (AO) adalah 2:1, dan pH optimum 2,97. Validasi metode menunjukkan nilai linearitas (r)0,998, batas deteksi 0,0343 mg/L, batas kuantifikasi 0,104 mg/L, presisi 0,382-1,78 %, sensitivitas 4,64 x 104 L mol-1cm-1 dan akurasi 82,11-100,3%.

 

 

Keywords

Validasi, surfaktan, acridine orange, lele

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