Aktivitas Antibakteri Ekstrak Bunga Telang (Clitoria ternatea L.) terhadap Bakteri Patogen

Febrianti Febrianti, Asri Widyasanti, Siti Nurhasanah

Abstract

Penyakit manusia dapat disebabkan oleh kontribusi bakteri yang menyebabkan infeksi. Penggunaan tanaman sebagai pengobatan tradisional memainkan peran penting dalam kesehatan karena dianggap sebagai salah satu sumber yang paling menjanjikan untuk penemuan agen antimikroba baru yang minim efek samping. Bunga telang mulai dilirik masyarakat Indonesia sebagai bunga dengan berbagai macam manfaat bagi kesehatan manusia salah satunya sebagai antibakteri, namun sayangnya belum diketahui seberapa jauh manfaat tersebut. Penelitian ini bertujuan untuk mengeksplorasi kandungan senyawa dan studi antibakteri ekstrak bunga telang menggunakan beberapa pelarut bersama dengan penilaian kritis terhadap potensinya di masa depan. Metode penelitian dilakukan dengan metode penelusuran literatur. Senyawa metabolit sekunder yang ditemukan pada ekstrak bunga telang seperti flavonoid, alkaloid, fenol, saponin dan tanin bertanggungjawab atas aktivitas antibakteri. Ekstrak bunga telang terbukti memberikan aktivitas antibakteri terhadap beberapa bakteri patogen penyebab konjungtivis, keracunan makanan, infeksi saluran kemih, infeksi kulit dan kerusakan gigi. Bakteri patogen seperti P. aeruginosa, E. coli, dan S. aureus berhasil dihambat pertumbuhannya dengan nilai Diameter Daya Hambat (DDH) 8 ‒ 26 mm. Aktivitas antibakteri yang dimilikinya dipengaruhi oleh pelarut yang digunakan, ekstrak metanol dengan konsentrasi 200 mg/mL memberikan aktivitas antibakteri yang paling kuat terhadap P. aeruginosa dengan nilai DDH 26 mm yang tergolong penghambatan kategori kuat. Hal ini membuktikan ekstrak bunga telang memiliki potensi untuk dikembangkan sebagai antibakteri alami.

Antibacterial Activity of Clitoria ternatea L. Extract against Pathogenic Bacteria. Human disease can be caused by bacteria that cause infections. The use of plants as traditional has played an important role in health because it is considered as one of the most promising sources for the discovery of new antimicrobial agents with minimal side effects. Recently, C. ternatea has attracted much attention for Indonesian people as a flower with various benefits for human health, one of which is as an antibacterial. However, how far these benefits are still being investigated. For this reason, this study aims to explore the compound content and antibacterial studies of C. ternatea extract using several solvents along with a critical assessment of its potential in the future. The research method used was the literature search method. Secondary metabolite compounds found in C. ternatea extracts, such as flavonoids, alkaloids, phenols, saponins, and tannins, are responsible for the antibacterial activity. C. ternatea extract has antibacterial activity against several pathogenic bacteria that cause conjunctivitis, food poisoning, urinary tract infections, skin infections, and tooth decay. Pathogenic bacteria such as P. aeruginosa, E. coli, and S. aureus were inhibited with an inhibition zone value of 8 ‒ 26 mm. Its antibacterial activity is influenced by the solvent used, methanol extract with a concentration of 200 mg/mL gave the strongest activity against P. aeruginosa with an inhibition zone of 26 mm, classified as a strong inhibitory category. This proves that the C. ternatea extract has the potential to be further developed as a natural antibacterial agent.

Keywords

antibacterial activity; butterfly pea; Clitoria ternatea L.; butterfly pea extract.

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References

Angriani, L., 2019. Potensi Ekstrak Bunga Telang (Clitoria Ternatea) Sebagai Pewarna Alami Lokal Pada Berbagai Industri Pangan. Canrea Journal: Food Technology, Nutritions, and Culinary Journal 2, 32–37. doi: 10.20956/canrea.v2i1.120.

Bérdy, J., 2005. Bioactive Microbial Metabolites. The Journal of Antibiotics 58, 1–26. doi: 10.1038/ja.2005.1.

Brooks, G.F., Butel, J.S., and Morse, S.A., 2008. Jawetz, Melnick, & Adelberg: Mikrobiologi kedokteran, 23rd ed. EGC, Jakarta: Sunter Agung Podomoro.

Budiasih, K.S., 2017. Kajian Potensi Farmakologis Bunga Telang (Clitoria Ternatea L.), in: Prosiding Seminar Nasional Kimia UNY 2017. 201–206.

Cahyaningsih, E., Yuda, P.E.S.K., and Santoso, P., 2019. Skrining Fitokimia Dan Uji Aktivitas Antioksidan Ekstrak Etanol Bunga Telang (Clitoria Ternatea L.) Dengan Metode Spektrofotometri UV-VIS. Jurnal Ilmiah Medicamento 5, 51–57. doi: 10.36733/medicamento.v5i1.851.

Cowan, M.M., 1999. Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews 12, 564–582. doi: 10.1128/CMR.12.4.564.

Devi, B.P., and Ramasubramaniaraja, R., 2009. Dental Caries and Medicinal Plants–An Overview. Journal of Pharmacy Research 2, 1669–1675.

Friedman, N.D., Temkin, E., and Carmeli, Y., 2016. The Negative Impact of Antibiotic Resistance. Clinical Microbiology and Infection 22, 416–422. doi: 10.1016/j.cmi.2015.12.002.

Ginovyan, M., Petrosyan, M., and Trchounian, A., 2017. Antimicrobial Activity of Some Plant Materials Used in Armenian Traditional Medicine. BMC Complementary and Alternative Medicine 17, 50. doi: 10.1186/s12906-017-1573-y.

Hidayati, W., Yuniarti, F., Shofaya, L., and Utomo, S.P., 2017. Screening And Identification Endophytic Bacteria From Indonesian Bay Leaves (Eugenia Polyantha Wight) with Antibacteria Activity, in: Proceeding Kolokium UHAMKA. pp. 167–176.

Hurdle, J.G., O’Neill, A.J., Chopra, I., and Lee, R.E., 2011. Targeting Bacterial Membrane Function: An Underexploited Mechanism for Treating Persistent Infections. Nature Reviews Microbiology 9, 62–75. doi: 10.1038/nrmicro2474.

Jamil, N., and Pa’Ee, F., 2018. Antimicrobial Activity from Leaf, Flower, Stem, and Root of Clitoria Ternatea - A Review, in: AIP Conference Proceedings. pp. 1–6. doi: 10.1063/1.5050140.

Kamilla, L., Mnsor, S.M., Ramanathan, S., and Sasidharan, S., 2009. Antimicrobial Activity of Clitoria Ternatea (L.) Extracts. Pharmacologyonline 1, 731–738.

Kazuma, K., Noda, N., and Suzuki, M., 2003. Flavonoid Composition Related to Petal Color in Different Lines of Clitoria Ternatea. Phytochemistry 64, 1133–1139. doi: 10.1016/S0031-9422(03)00504-1.

Khumairoh, L., Susilo, J., and Vifta, R.L., 2020. Perbedaan Pelarut Etanol 96% Dan Etil Asetat Pada Uji Aktivitas Antibakteri Ekstrak Bunga Telang (Clitoria Ternatea L.) Terhadap Propionibacterium Acnes. Universitas Ngudi Waluyo, 1–6.

Kurniati, N.F., Garmana, A.N., and Aziz, N., 2017. Aktivitas Antibakteri Dan Antijamur Ekstrak Etanol Akar, Bunga, Dan Daun Turi (Sesbania Grandiflora L. Poir). Acta Pharmaceutica Indonesia 42, 1–8.

Manjula, P., Mohan, C.H., Sreekanth, D., Keerthi, B., and Devi, B.P., 2013. Phytochemical Analysis of Clitoria Ternatea Linn., a Valuable Medical Plant. Journal of the Indian Botanical Society 92(374), 173-178.

Marpaung, A.M., 2020. Tinjauan Manfaat Bunga Telang (Clitoria Ternatea L.) Bagi Kesehatan Manusia. Journal of Functional Food and Nutraceutical 1, 63–85. doi: 10.33555/jffn.v1i2.30.

Mukherjee, P.K., Kumar, V., Kumar, N.S., and Heinrich, M., 2008. The Ayurvedic Medicine Clitoria Ternatea-From Traditional Use to Scientific Assessment. Journal of Ethnopharmacology 120, 291–301. doi: 10.1016/j.jep.2008.09.009.

Gowd, M.J.S.P., Kumar, M.G.M., Shankar, A.J.S., Sujatha, B., and Sreedevi, E., 2012. Evaluation of Three Medicinal Plants for Anti-Microbial Activity. AYU (An International Quarterly Journal of Research in Ayurveda) 33, 423. doi: 10.4103/0974-8520.108859.

Riyanto, E.F., Nurjanah, A. N., Ismi, S. N., and Suhartati, R., 2019. Daya Hambat Ekstrak Etanol Bunga Telang (Clitoria Ternatea L.) Terhadap Bakteri Perusak Pangan. Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-ilmu Keperawatan, Analis Kesehatan dan Farmasi 19(2), 218–225. doi: 10.36465/jkbth.v19i2.500.

Rota, M.C., Herrera, A., Martínez, R.M., Sotomayor, J.A., and Jordán, M.J., 2008. Antimicrobial Activity and Chemical Composition of Thymus Vulgaris, Thymus Zygis and Thymus Hyemalis Essential Oils. Food Control 19, 681–687. doi: 10.1016/j.foodcont.2007.07.007.

Santos, I.P. dos, Silva, L.C.N. da, Silva, M.V. da, Araújo, J.M. de, Cavalcanti, M. da S., and Lima, V.L. de M., 2015. Antibacterial Activity of Endophytic Fungi from Leaves of Indigofera Suffruticosa Miller (Fabaceae). Frontiers in Microbiology 6, 1–7. doi: 10.3389/fmicb.2015.00350.

Sumartini, Ikrawan, Y., and Muntaha, F.M., 2020. Analisis Bunga Telang ( Clitoria Ternatea ) Dengan Variasi PH Meode High Performance Liquid Chromatograph-Tandem Mass Spectrometry (LC-MS/MS). Pasundan Food Technology Journal 7, 70–77. doi: 10.23969/pftj.v7i2.2983.

Terahara, N., Toki, K., Saito, N., Honda, T., Matsui, T., and Osajima, Y., 1998. Eight New Anthocyanins, Ternatins C1-C5 and D3 and Preternatins A3 and C4 from Young Clitoria Ternatea Flowers. Journal of Natural Products 61, 1361–1367. doi: 10.1021/np980160c.

Uma, B., Prabhakar, K., and Rajendran, S., 2009. Phytochemical Analysis and Antimicrobial Activity of Clitorea Ternatea Linn against Extended Spectrum Beta Lactamase Producing Enteric and Urinary Pathogens. Asian Journal of Pharmaceutical and Clinical Research 2, 94–96.

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