Soerya Dewi Marliyana, Yana Maolana Syah, Didin - Mujahidin


In vitro antibacterial activity of chalcone derivatives from “temu kunci” (K. pandurta) rhizomes against clinical isolate bacteria has been done. Two chalcone derivatives, panduratin A (1) and 4-hydroxypanduratin A (2) were isolated from Kaempferia pandurata rhizomes. Isolation of the chemical components were done with extraction (maceration), vacuum liquid chromatography and radial chromatography methods. The structures were determined by NMR spectroscopy (1H-NMR, 13C-NMR, 1D and 2D), then compare with data from literatures. Antibacterial activity was carried out with reference to the CLSI microdilution method, against eight clinical isolate bacteria such as Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysentriae and Vibrio cholerae. Compounds 1 and 2 showed significant antibacterial activity with highest activity against S. aureus and B. subtilis with MIC values of 2.4 to 18.8 µg/mL and MBC values of 4.8 to 37.5 µg/mL. These results showed these compounds as potential antibacterial agent for clinical isolate bacteria.


clinical isolate bacteria; antibacterial activity; panduratin A; 4-hydroxypanduratin A

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Arakawa, H., Maeda, M., Okubo, S., and Shimamura,T., 2004. Role of hydrogen peroxide in bactericidal action of catechin. Biological and Pharmaceutical Bulletin 27, 277–281.

Calliste, C.A., Le Bail, J.C., Trouillas, P., Pouget, C., Habrioux, G., Chulia, A. J., and Duroux, J. L., 2001, Chalcones: structural requirements for antioxidant, estrogenic and antiproliferative activities, Anticancer Research, vol. 21, pp. 3949–3956.

Cheenpracha, S., Karalai, C., Ponglimanont, C., Subhadhirasakul, S., and Tewtrakul, S., 2006. Anti-HIV-1 protease activity of compounds from Boesenbergia pandurata. Bioorganic and Medicinal Chemistry 14 (6), 1710–1714.

Chinnam, N., Dad, P. K., Sabri, S. A., Ahmad, M., Kabir, M. A., and Ahmad, Z., 2010. Dietary bioflavonoids inhibit Escherichia coli ATP synthase in a differential manner. International Journal of Biological Macromolecules 46, 478–486.

Clinical and Laboratory Standards Institute, 2012. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard-9th edition. CLSI Document M07-09 32 (2), Wayne, PA, USA.

Cushnie, T. P. T., Lambb, A. J., 2011. Review: Recent advances in understanding the antibacterial properties of flavonoids, International Journal of Antimicrobial Agents, 38, 99– 107.

Gradisar, H., Pristovsek, P, Plaper, A, and Jerala, R., 2007. Green tea catechins inhibit bacterial DNA gyrase by interaction with its ATP binding site. Journal of Medicinal Chemistry 50, 264–271.

Hwang, J. K., Chung, J.Y., Baek, N. I., and Park, J. H., 2004. Isopanduratin A from Kaempferia pandurata as an active antibacterial agent against cariogenic Streptococcus mutans. International Journal of Antimicrobial Agents 23, 377–381.

Marliyana, S. D., Rukayadi, Y., Ismail, I. S., Mujahidin, D., and Syah, Y. M., 2015. Inhibitory properties of panduratin A and 4-hydroxypanduratin A isolated from Kaempferia pandurata against some pathogenic bacteria. Current Topics in Toxicology 11, 23-28.

Pandji, C., Grimm, C., Wray, V., Witte, L., and Proksch, P., 1993. Insecticidal constituents from four species of the zingiberaceae. Phytochemistry 34, 415–419.

Park, K. M., Choo, J. H., Sohn, J. H., Lee, S. H., and Hwang, J. K., 2005. Antibacterial activity of panduratin A isolated from Kaempferia pandurata against Porphyromonas gingivalis. Food Science and Biotechnology 14, 286–289.

Poerwono, H., Sasaki, S., Hattori, Y., and Higashiyama, K., 2010. Efficient microwave-assisted prenylation of pinostrobin and biological evaluation of its derivatives as antitumor agents. Bioorganic and Medicinal Chemistry Letters 20, 2086–2089.

Rukayadi, Y., Han, S., Yong, D., and Hwang, J. K., (2010. In vitro antibacterial activity of panduratin A against Enterococci clinical isolates. Biological and Pharmaceutical Bulletin 33 (9), 1489-1493.

Shindo, K., Kato, M., Kinoshita, A., Kobayashi, A., Koike, Y., 2006. Analysis of antioxidant activities contained in the Boesenbergia pandurata Schult. Rhizome. Bioscience, Biotechnology and Biochemistry 70, 2281–2284.

Tewtrakul, S., Subhadhirasakul, S., Karalai, C., Ponglimanont, C., and Cheenpracha, S., 2009. Anti-inflammatory effects of compounds from Kaempferia parviflora and Boesenbergia pandurata, Food Chemistry 115 (2), 534-538.

Trakoontivakorn, G., Nakahara, K., Shinmoto, H., Takenaka, M.,Onishi-Kameyama, M., Ono, H., Yoshida, M., Nagata, T., and Tsushida, T., 2001. Structural analysis of a novel antimutagenic compound, 4-hydroxypanduratin A, and antimutagenic activity of flavonoids in a Thai spice, fingerroot (Boesenbergia pandurata Schult.) against mutagenic heterocyclic amines. Journal of Agricultural and Food Chemistry 49, 3046–3050.

Tuchinda, P., Reutrakul, V., Claeson, P., Ponprayoon, U., Sematong, T., Santosuk, T., and Taylor, W. C., 2002. Anti-inflammatory cyclohexenyl chalcone derivatives in Boesenbergia pandurate. Phytochemistry 59, 169–173.

Win, N. N., Awale, S., Esumi, H., Tezuka, Y., and Kadota, S., 2007. Bioactive secondary metabolites from Boesenbergia pandurata of Myanmar and their preferential cytotoxicity against Human Pancreatic Cancer PANC-1 cell line in nutrient-deprived medium. Journal of Natural Products 70, 1582–1587.


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