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Aktivitas Antioksidan dan Screening Fitokimia Kulit Bligo (Benincasa hispida)
Abstract
Bligo (Benincasa hispida) merupakan tanaman yang tumbuh di daerah tropis dan subtropis. Buah bligo mengandung senyawa-senyawa aktif antara lain flavonoid, glikosida, karoten, vitamin, mineral, dan asam uronat. Penelitian di bidang farmakologi telah mengungkapkan bahwa buah bligo memberikan efek kesehatan, antara lain antidepressant, ansiolitik, antioksidan, antiinflamatori, diuretik, antidiabetik, dan antimikrobia. Namun penelitian di Indonesia mengenai kulit bligo masih sangat terbatas. Penelitian ini bertujuan untuk melakukan aktivitas antioksidan dan screening fitokimia pada kulit bligo. Kulit bligo dikeringkan kemudian digiling menjadi bubuk ukuran 100 mesh. Pengujian yang dilakukan adalah uji aktivitas antioksidan menggunakan DPPH, dan uji screening fitokimia kualitatif menggunakan LC-MS/MS QTOF. Aktivitas antioksidan pada kulit bligo adalah kelas medium dengan nilai IC50 6,91 mg/mL. Dari hasil analisis LC-MS/MS QTOF diketahui bahwa kulit bligo mengandung 36 senyawa, dengan 5 senyawa dominannya adalah adenosin; polidatin; 7-(4-hidroksi-3-metoksifenil)-l-(4-hidroksifenil)-4E,6E-heptadien-3-on; morasin C; dan kushenol S. Hasil karakterisasi yang diperoleh menunjukkan bahwa kulit bligo berpotensi sebagai senyawa aktif.
Antioxidant Activity and Phytochemical Screening of Peel Bligo (Benincasa hispida). Bligo (Benincasa hispida) is a plant that grows in tropical and subtropical regions. Bligo fruit contains active compounds such as flavonoids, glycosides, carotene, vitamins, minerals, and uronic acid. Research in the field of pharmacology has revealed that bligo fruit has health effects, including antidepressants, anxiolytics, antioxidants, anti-inflammatory, diuretics, antidiabetic, and antimicrobials. However, research on bligo peel is very limited. This study aims were to carry out antioxidant activity and phytochemical screening of bligo peel characteristics. Bligo’s peel was dried and ground into 100 mesh powder. Then antioxidant activity test using DPPH, and qualitative phytochemical screening test using LC-MS/MS QTOF were conducted. The bligo peel is medium class of antioxidant activity with IC50 value was 6.91 mg/mL. From the results of LC-MS/MS QTOF analysis it was known that bligo skin contained 36 compounds, with 5 dominant compounds, namely adenosine; polydatin; 7-(4-hydroxy-3-methoxyphenyl)-1-(4-hydroxyphenyl)-4E, 6E-heptadien-3-one; moracin C; and kushenol S. The results of the characterization showed that bligo skin has the potential as a source of active compounds for further utilization.
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