Determination of Vitamin C in Various Colours of Bell Pepper (Capsicum annuum L.) by Titration Method

Nerdy Nerdy


Vitamin C is needed by human body to improve the immune system and can be obtained from the bell pepper. Bell pepper has a different color according to the level of maturity, ranging from green, yellow, orange, and red. Differences in color also make possible differences in vitamin C content. The purpose of this study was to determine vitamin C levels in various colors of bell fruit. Measurement of vitamin C was done by titration method with metaphosphoric acid as the solvent and 2,6 dichloroindophenol as the standardized solution. The samples used were green bell pepper, yellow bell pepper, orange bell pepper, and red bell pepper taken from Gundaling, Berastagi, Karo, Sumatera Utara, 22152, Indonesia. The results show that vitamin C level in various colors of bell pepper respectively, for green bell pepper 16.52 mg vitamin C per 100 g green bell pepper; yellow bell pepper 159.61 vitamin C per 100 g yellow bell pepper; orange bell pepper 121.38 vitamin C per 100g orange bell pepper; red bell pepper 81.19 vitamin C per 100 g red bell pepper. These results indicate that different levels of bell pepper maturity have different vitamin C content.


vitamin C; various colors; bell Pepper; Capsicum annuum; titration

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Al Majidi, M.I.H., and Al Qubury, H.Y. 2016. Determination of Vitamin C (Ascorbic Acid) Contents in Various Fruit and Vegetable by UV Spectrophotometry and Titration Methods. Journal of Chemical and Pharmaceutical Sciences 9(4), 2972-2974.

Bekele, D.A., and Geleta, G.S. 2015. Iodometric Determination of the Ascorbic Acid (Vitamin C) content of some Fruits consumed in Jimma Town Community in Ethiopia. Research Journal of Chemical Sciences 5(1), 60-63.

Bieniasz, M., Dziedzic, E., and Kaczmarczyk, E. 2017. The Effect of Storage and Processing on Vitamin C Content in Japanese Quince Fruit. Folia Horticulturae 29(1), 83-93.

Counsel, J.N. and Hornig, D.H. 1981. Vitamin C. London and New Jersey: Applied Science Publishers.

Djoković, G.A., Pavlović, R., Mladenović, J., and Djurić, M. 2011. Vitamin C Content in Different Types of Lettuce Varieties. Acta Agriculturae Serbica 16(32), 83-89.

El Ishaq, A., and Obirinakem, S. 2015. Effect of Temperature and Storage on Vitamin C Content in Fruits Juice. International Journal of Chemical and Biomolecular Science 1(2), 17-21.

Garcia, P.L., Buffoni, E., Gomes, F.P., and Quero, J.L.V. 2011. Analytical Method Validation, Wide Spectra of Quality Control. Dr. Isin Akyar (Ed.). ISBN: 978-953-307-683-6. InTech. Available from: spectra-of-quality-control/analytical-method-validation.

Gazdik, Z., Zitka, O., Petrlova, J., Adam, V., Zehnalek, J., Horna, A., Reznicek, V., Beklova, M., and Kizek, R. 2008. Determination of Vitamin C (Ascorbic Acid) Using High Performance Liquid Chromatography Coupled with Electrochemical Detection. Sensors 8(1), 7097-7112.

Harmita. 2004. Method Validation Implementation Guidelines and Calculations. Jakarta, Indonesia: Department of Pharmacy, Faculty of Mathematics and Natural Sciences, University of Indonesia.

Horwitz, W. 2003. Official Methods of Analysis of the Association of Official Analytical Chemists. 17th Edition. Washington: Association of Analytical Chemists.

Huber, L. 2007. Validation and Qualification in Analytical Laboratories. New York: Interpharm Informa Healthcare.

Hussain, M.I., Hamza, A., and Rashid, M.A. 2014. Estimation of Vitamin C in Carrot Before Cooking and After Cooking. Journal of Food and Nutrition Sciences 4(4), 108-112.

Ibrahim, M.A. 2016. Effect of Different Storage Condition on pH and Vitamin C Content in Some Selected Fruit Juices (Pineapple, Pawpaw and Watermelon). International Journal of Biochemistry Research & Review 11(2), 1-5.

Lee, S.K., and Kader, A.A. 2000. Preharvest and Postharvest Factors Influencing Vitamin C Content of Horticultural Crops. Postharvest Biology and Technology 20(1), 207-220.

Mateos, R.M., Jiménez, A., Román, P., Romojaro, F., Bacarizo, S., Leterrier, M., Gómez, M., Sevilla, F., del Río, L.A., Corpas, F.J., and Palma, J.M. 2013. Antioxidant Systems from Pepper (Capsicum annuum L.) - Involvement in the Response to Temperature Changes in Ripe Fruits. International Journal of Molecular Sciences

(1), 9556-9580.

Méndez, R.F., and Arancibia, S.R. 2015. Vitamin C in Health and Disease: Its Role in the Metabolism of Cells and Redox State in the Brain. Frontiers in Physiology 6(1), 397-407.

Mendoza, C.C., Sanchez, E., Marquez, E.M., Arreola, J.P.S., and Cordova, M.A.F. 2015. Bioactive Compounds and Antioxidant Activity in Different Grafted Varieties of Bell Pepper. Antioxidants 4(1), 427-446.

Moneruzzaman, K.M., Hossain, A.B.M.S., Sani, W., and Saifuddin, M. 2008. Effect of Stages of Maturity and Ripening Conditions on the Biochemical Characteristics of Tomato. American Journal of Biochemistry and Biotechnology 4(4), 336-344

Muhammad, I., Ashiru, S., Ibrahim, I.D., Kanoma, A.I., Sani, I., and Garba, S. 2014. Effect of Ripening Stage on Vitamin C Content in Selected Fruits. International Journal of Agriculture, Forestry and Fisheries 2(3), 60-65

Nadeem, M., Anjum, F.M., Khan, M.R., Saeed, M., and Riaz, A. 2011. Antioxidant Potential of Bell Pepper (Capsicum annum L.) - A Review. Pakistan Journal of Food Sciences 21(1-4), 45-51

Najwa, R.F., and Azrina, A. 2017. Comparison of Vitamin C Content in Citrus Fruits by Titration and High Performance Liquid Chromatography (HPLC) Methods. International Food Research Journal 24(2), 726-733.

Njoku, P.C., Ayuk, A.A., Okoye, C.V. 2011. Temperature Effects on Vitamin C Content in Citrus Fruits. Pakistan Journal of Nutrition 10(12), 1168-1169.

Oyetade, O.A., Oyeleke, G.O., Adegoke, B.M., and Akintunde, A.O. 2012. Stability Studies on Ascorbic Acid (Vitamin C) From Different Sources. IOSR Journal of Applied Chemistry 2(4), 20-24.

Padrón, R.A.R., Guedes, J.V.C., Swarowsky, A., Nogueira, C.U., Cerquera, R.R., and Pérez, J.C.D. 2015. Supplemental Irrigation Levels in Bell Pepper under Shade Mesh and in Open Field - Crop Coefficient, Yield, Fruit Quality and Water Productivity. African Journal of Agricultural Research 10(44), 4117-4125.

Pacier, C., and Martirosyan, D.M. 2015. Vitamin C - Optimal Dosages, Supplementation and Use in Disease Prevention. Functional Foods in Health and Disease 5(3), 89-

Patrick, A.O., Fabian, U.A., Peace, I.C., and Fred, O.O. 2016. Determination of Variation of Vitamin C Content of Some Fruits and Vegetables Consumed in Ugbokolo After Prolonged Storage. IOSR Journal of Environmental Science, Toxicology and Food Technology 10(7), 17-19.

Pessarakli, M. 2011. Handbook of Plant and Crop Stress. 3rd Edition. New York: CRC Press.

Pisoschi, A.M., Danet, A.F., and Kalinowski, A. 2008. Ascorbic Acid Determination in Commercial Fruit Juice Samples by Cyclic Voltammetry. Journal of Automated Methods and Management in Chemistry 2008(1), 1-8.

Pisoschi, A.M., Pop, A., Negulescu, G.P., and Pisoschi, A. 2011. Determination of Ascorbic Acid Content of Some Fruit Juices and Wine by Voltammetry Performed at Pt and Carbon Paste Electrodes. Molecules 16(1), 1349-1365.

Ruvini, L., Dissanayaka, W.M.M.M.K., Chathuni, J., Rizliya, V., Swarna, W., and Barana, C.J. 2017. Effect of Different Drying Methods on Antioxidant Activity of Star Fruits (Averrhoa carambola L.). Journal of Nutrition and Diet Supplements

(1), 1-6

Shrestha, N., Shrestha S., and Bhattarai, A. 2016. Determination of Ascorbic Acid in Different Citrus Fruits of Kathmandu Valley. Journal of Medical and Biological Science Research 2(1), 9-14

Singh, V., and Kumari, M. 2015. Affets of Ascorbic Acid on Health - A Review Article. International Journal of Applied Home Science 2(9&10), 289-297.

Tantray, A.K., Dar, S.A., Ahmad, S., and Bhat, S.A. 2017. Spectrophotometric and Titrimetric Analysis of Phytoascorbate. Journal of Pharmacognosy and Phytochemistry 6(1), 27-31.

Tareen, H., Ahmed, S., Mengal, F., Masood, Z., Bibi, S., Mengal, R., Shoaib, S., Irum, U., Akbar. S., Mandokhail, F., and Taj, R. 2015. Estimation of Vitamin C Content in Artificially Packed Juices of Two Commercially Attracted Companies in Relation to Their Significance for Human Health. Biological Forum an International Journal 7(2), 682-685.

Tosun, I., Ustun, N.S., and Tekguler, B. 2008. Physical and Chemical Changes during Ripening of Blackberry Fruits. Scientia Agricola 65(1), 87-90.

Trani, M.T.T., Phillips, K.M., and Cotty, M. 2012. Matrix Specific Method Validation for Quantitative Analysis of Vitamin C in Diverse Foods. Journal of Food Composition and Analysis 26(1), 12-25.

Ullah, S., Hussain, A., Ali, J., Khaliqurrehman, and Ullah, A. 2012. A Simple and Rapid HPLC Method for Analysis of Vitamin C in Local Packed Juices of Pakistan. Middle East Journal of Scientific Research 12(8), 1085-1091.

Zhigila, D.A., Rahaman, A.A.A., Kolawole, O.S., and Oladele, F.A. 2014. Fruit Morphology as Taxonomic Features in Five Varieties of Capsicum annuum L. Solanaceae. Journal of Botany 2014(1), 1-6.