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Aktivitas Antibakteri dan Antioksidan Asam Galat dari Kulit Buah Lokal yang Diproduksi dengan Tanase
Abstract
Penelitian ini mempelajari tentang produksi asam galat menggunakan enzim tanase dari limbah kulit buah lokal di Lombok. Limbah kulit buah lokal yang digunakan yaitu kulit buah kepundung (Baccaurea racemosa Muell.Arg), kulit buah juwet (Syzygium cumini), dan kulit buah manggis (Garcinia mangostana). Pada penelitian ini dilakukan pengujian asam galat yang dihasilkan, untuk mengetahui aktivitas antioksidan dan antibakteri. Pengujian sifat antioksidan dilakukan dengan menggunakan metode DPPH, sedangkan aktivitas antibakteri diuji dengan menggunakan metode difusi agar pada bakteri Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa asam galat dari ketiga kulit buah memiliki aktivitas antioksidan. Asam galat dari kulit buah kepundung, kulit buah juwet dan kulit buah manggis memiliki IC50 masing-masing sebesar 5.95; 5,96; dan 5,92 ppm. Berbeda dengan kemampuan sebagai antioksidan, asam galat resistan terhadap bakteri Escherichia coli dan Staphylococcus aureus yang menunjukkan bahwa asam galat tidak memiliki aktivitas sebagai antibakteri.
Antibacterial and Antioxidant Activity of Gallic Acid Extracted from Local Fruit Peels Produced by Tannase. This research studied the gallic acid production using tannase enzym from fruit local peels in Lombok. The local fruit waste used are kepundung rind (Baccaurea racemosa Muell.Arg), juwet rind (Syzygium cumini) and mangosteen rind (Garcinia mangostana). The produced Gallic acid was tested to determine its antioxidant and antibacterial activity. The antioxidant test conducted by DPPH method, meanwhile, the antibacterial activity was performed by agar diffusion method against bacteria Escherichia coli and Staphylococcus aureus. The results showed that the produce gallic acid from those three rind provide antioxidant activity. Gallic acid from kepundung rind, juwet rind, and mangosteen rind have IC50 values of 5,95; 5,96; and 5,92, respectively. Gallic acid does not showed inhibitory actions against the growth of Escherichia coli and Staphylococcus aureus. This result showed that gallic acid from local fruit waste provided no an antibacterial activity.
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Ahmeda, A., Hossain, M.A., and Ismail, Z., 2009. Antioxidant Properties of the Isolated Flavonoids from the Medicinal Plant Phyllanthus niruri. Asian Journal of Food and Agro-Industry 2(3), 373-381.
Al-Zahrani, S.H.M., 2012. Antibacterial Activities of Gallic Acid and Gallic Acid Methyl Ester on Methicillin-Resistant Staphylococcus aureus. Journal of American Science 8(2), 7–12.
Anwar, Y.A.S., and Burhanuddin., 2012. Pengaruh Komposisi Media terhadap Aktivitas dan Karakter Enzim Tanin Asil Hidrolase dari Aspergillus niger. Jurnal Ilmu Kefarmasian Indonesia 10 (2), 87-92.
Anwar, Y.A.S., Artika, I.M., and Danuri, H., 2007. The Production of Tannin Acyl Hydrolase from Aspergillus niger. Mikrobiologi Indonesia 1(2), 91-94.
Badhani, B., Sharma, N., and Kakkar, R., 2015. Gallic Acid: a Versatile Antioxidant with Promising Therapeutic and Industrial Applications. RSC Adv 5, 27540-27557.
Bajpai, B., and Patil, S., 2008. A New Approach to Microbial Production of Gallic Acid. Brazilian Journal of Microbiology 39, 708-711.
Banerjee, D., Debdulal., and Pati, B.R., 2007. Optimization of Tannase Production by Aureobasidium pullulans DBS66. Journal of Microbiology and Biotechnology 17, 1049-1053.
Belur, P.D., and Pallabhanvi, B., 2011. Investigation on Production of Gallic Acid From Terminalia chebula Extract Using Cell Associated Tannase of Bacillus massiliensin. International Conference of Advances in Biotechnology and Pharmaceutical Sciences (ICABPS): Bangkok.
Beniwal, V., Chhokar, V., Singh, N., and Sharma, J., 2010. Optimization of Process Parameters for the Production of Tannase and Gallic Acid by Enterobacter cloacae MTCC 9125. Journal of American Science 6(8), 389–397.
Bhagat, S., Dongre, P., Bhagat, S., and Dikpati, A., 2015. Extraction and Isolation of Gallic Acid from Self-Generated Fermentation System of Terminalia chebula. Journal of Chemical and Pharmaceutical Research 7(4), 170–174.
Borra, S.K., Gurumurthy, P., Mahendra, J., K.M. Jayamathi, C.N. Cherian, and Chand, R., 2013. Antioxidant and Free Radical Scavenging Activity of Curcumin Determined by Using Different in Vitro and ex Vivo Models. Journal of Medicinal Plants Research 7(36), 2680-2690.
Brighente, I.M.C., Dias, M., Verdi, L.G., and Pizzolatti, M.G., 2007. Antioxidant Activity and Total Phenolic Content of Some Brazilian Species. Pharmaceutical Biology 45(2), 156-161.
Chia, Y-C., Rajbanshi, R., Calhoun, C., and Chiu, R.H., 2010. Anti-Neoplastic Effects of Gallic Acid a Major Component of Toona Sinensis Leaf Extract on Oral Squanous Carcinoma Cell. Molecules 15, 8377-8389.
Changzhong, L., Chen, C., Mo H, Ma H, Yuan E, and Li Q., 2014. Characterization and DPPH Radical Scavenging Activity of Gallic Acid-Lecithin Complex. Tropical Journal of Pharmaceutical Research 13(8), 1333-1338.
Chung, K.T., Stevens, S.E., Lin, W.F., and Wei, C.I., 1993. Growth Inhibition of Selected Food-Borne Bacteria by Tannic Acid, Propyl Gallate and Related Compounds. Letter in Applied Microbiology 17, 29-32.
Gaffar, R., Sazali, N.E.S., and Abdul Majid, F.A., 2008. Colour Reduction and Anti- Microbial Evaluation of Pre-Treated Cashew Leaves Extract. Journal of Chemical and Natural Resources Engineering - Sustainable Process for Natural Resources 2, 1-9.
Hagerman, A.E., 2002. Tannin Chemistry. http://www.users.muohio .edu/ hagermae / tanin.pdf (diakses pada 9 Juli 2016).
Hajimehdipoor, H., Shahrestani, R., and Shekarchi, M., 2014. Investigating the Synergistic Antioxidant Effects of Some Flavonoid and Phenolic Compounds. Research Journal of Pharmacognosy 1(3), 35-40.
Iqbal, H., and Kapoor, A., 2012. Culture conditions for the production of tannase from Tricoderma harzianum MTCC 10841. International Journal of Science and Technology 1(2), 584-595.
Jindal, H.M.K., and Mohamad, J., 2012. Antioxidant Activity of Ardisia Crispa (Mata Pelanduk). Sains Malaysiana 41(5), 539-545.
Junaidi, E., and Anwar, Y.A.S., 2015. Produksi Asam Galat dari Limbah Buah Lokal Di Pulau Lombok secara Enzimatis. Laporan Penelitian Hibah Bersaing Kemenristek.
Kasture, V.S., Katti, S.A., Mahajan, D., Wagh, R., Mohan, M., and Kasture, S.B., 2009. Antioxidant and Antiparkison Activity of Gallic Acid Derivatives.
Pharmacologyonline 1, 385-395.
Kosem, N., Han,Y-H., and Moongkarndi, P., 2007. Antioxidant and Cytoprotective Activities of Metanolic Extract from Garcinia mangostana Hulls. Science Asia 33, 283–292.
Lokeswari, N., and Kumar, B.L., 2013. A Novel Biochemical Method for Production of an Antibacterial Drug Trimethoprim from Industrial Wastes. International Journal of Pharmacy and Pharmaceutical Sciences 5(3), 651–653.
Margaret, E., Shailaja, A.M., and Venugopal Rao, V., 2015. Evaluation of Antioxidant Activity in Different Parts of Syzygium cumini (Linn.). International Journal of Current Microbiology and Applied Sciences 4(9), 372-379.
Marino, T., Galano, A., and Russo, N., 2014. Radical Scavenging Ability of Gallic Acid Toward OH and OOH Radicals: Reaction Mechanism and Rate Constants from the Density Functional Theory. The Journal of Physical Chemistry B 118(35), 10380-10389.
Nutan et al., 2013. Ellagic Acid and Gallic Acid from Lagestroemia speciosa L. Inhibit HIV-1 Infection through Inhibition of HIV-1 Protease and Reverse Transcriptase Activity. Indian Journal of Medical Research 137, 540-548.
Palakawong, C., Sophanodora, P., Pisuchpen, S., and Phongpaichit, S., 2010. Antioxidant and Antimicrobial Activities of Crude Extracts from Mangosteen (Garcinia
mangostana L) Parts and Some Essential Oils. International Food Research Journal 17, 583–589.
Pedraza-Chaverri, J., Cardenas-Rodriguez, N., Orozco-Ibarra, M., and Perez-Rojas, J.M., 2008. Medicinal Properties of Mangosteen (Garcinia mangostana). Food and Chemical Toxicology 46, 3227– 3239.
Phansawan, B., and Pongsabangpho, S., 2014. Determination of Gallic Acid and Rutin in Extracts Cassia alata and Andrographis paniculata. Science Asia 40, 414–419.
Reginold Jebitta, S., and Jeyanth Allwin, S., 2016. Antioxidant Activity, Total Phenol, Flavonoid, and Anthocyanin Contents of Jamun (Syzygium Cumini) Pulp Powder. Asian Journal of Pharmaceutical and Clinical Research 9(2), 361–363.
Sari, P., Setiawan, A., and Siswoyo, T.A., 2015. Stability and Antioxidant Activity of Acylated Jambolan (Syzygiuoloum cumini) Anthocyanins Synthesized by Lipase- Catalyzed Transesterification. International Food Research Journal 22(2), 671-676.
Siti Azima, A.M., Noriham, A., and Manshoor, N., 2014. Anthocyanin Content in Relation to the Antioxidant Activity and Colour Properties of Garcinia mangostana Peel, Syzigium cumini and Clitoria ternatea Extracts. International Food Research Journal 21(6), 2369 –2375.
Tachakittirungrod, S., Okonogi, S., and Chowwanapoonpohn, S., 2007. Study on Antioxidant Activity of Certain Plants in Thailand: Mechanism of Antioxidant Action of Guava Leaf Extract. Food Chemistry 103, 381–388.
Vazirian, M., Khanavi, M., Amanzadeh, Y., and Hajimehdipoor, H., 2011. Quantification of Gallic Acid in Fruits of Three Medicinal Plants. Iranian Journal of Pharmaceutical Research 10(2), 233-236.
Wetwitayaklung, P., Charoenteeraboon, J., Limmatvapirat, C., and Phaechamud, T., 2012. Antioxidant Activities of Some Thai and Exotic Fruits Cultivated in Thailand. Research Journal of Pharmaceutical, Biological and Chemical Sciences 3(1), 12-21.
Zarena, A.S., and Sankar, K.U., 2009. A Study of Antioxidant Properties from Garzinia Mangostana L. Pericarp Extract. Acta Scientiarum Polonorum Technologia Alimentaria 8(1), 23–34.
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