Chemical Characterization and Microbiology of Khalas’s Date Vinegar (Phoenix dactylifera)

Devi Arianty, Nur Adawiyah Mahmud, Ernawati Ernawati

Abstract

Date vinegar is a processed date product that has a high nutritional content that improves the function of the body’s work system. This research aims to identify the chemical characterization and microbiology of date vinegar as an antimicrobial during the fermentation process. This study used a descriptive method for 30 days of the fermentation of date vinegar. The results were analyzed by a qualitative description of each parameter. Parameters observed were characterization of date vinegar compared to standard fermented vinegar according to SNI 01-4371-1996, organic acids, total phenols, and antimicrobial effect. The results indicated that the fermentation of Khalas dates for 30 days produced a pH of 4, 7.15% dissolved solids, 0% alcohol, 6% acetic acid, organic acid odor, liquid form, and dark brown color. The bioactive content of date vinegar was obtained using GC-MS a total of 44 compounds with two highest peaks were detected at RT 21.68 and RT 22.08. The resulting compounds are hexadecenoic methyl ester, Pentadecanoic acid, 14-methyl-, methyl ester dan Pentadecanoic acid, 13-methyl-, methyl ester, area at 19.98. On the other peaks are l-(+)-Ascorbic acid 2,6-dihexadecanoate, Estra-1,3, 5 (10)- trien-17β-ol, area at 9.79. Total phenol was 6213.57 mg/L, and the highest antimicrobial activity was with a concentration of 100% date vinegar of 15.27 mm.

Keywords

antimicrobial; bioactive; phenolic; date palm khalas

References

Aisyah, L. S., Jasmansyah, J., Purbaya, S., & Resnawati, T. (2019). Isolasi dan Uji Aktivitas Antibakteri Senyawa Fenol Ekstrak Etil Asetat Rimpang Jahe Merah (Zingiber officinale Roscoe var. sunti). Jurnal Kartika Kimia 2(1): 44–50. https://doi.org/10.26874/jkk.v2i1.23

Al-Farsi, M. A., & Lee, C. Y. (2008). Nutritional and functional properties of dates: A review. In Critical Reviews in Food Science and Nutrition 48(10): 877–887. https://doi.org/10.1080/10408390701724264

Al-Rousan, W. M., Olaimat, A. N., Osaili, T. M., Al-Nabulsi, A. A., Ajo, R. Y., & Holley, R. A. (2018). Use of acetic and citric acids to inhibit Escherichia coli O157:H7, Salmonella Typhimurium and Staphylococcus aureus in tabbouleh salad. Food Microbiology 73: 61–66. https://doi.org/10.1016/j.fm.2018.01.001

Arisandi, H., & Aminah, S. (2021). Pemanfaatan Limbah Kayu Praktikum dan Penelitian Untuk Pembuatan Cuka Kayu Sebagai Salah Satu Bahan Pembuatan Hand Sanitizer. Indonesian Journal of Laboratory 4(3): 82-89. https://doi.org/10.22146/ijl.v4i3.69273

Baharuddin, S., Syahidah, & Yatni, N. (2009). Penentuan Mutu Cuka Nira Aren (Arenga pinnata) Berdasarkan SNI 01-4371-1996. Jurnal Perennial 5(1), 31-35. https://doi.org/10.24259/perennial.v5i1.187

Bayu, M., Rizqianti, H., Nurwantoro. (2017). Analisis Total Padatan Terlarut, Keasaman, Kadar Lemak, dan Tingkat Viskositas pada Kefir Optima dengan Lama Fermentasi yang Berbeda. Jurnal Teknologi Pangan 1(2): 33-38. https://doi.org/10.14710/jtp.2017.17468

Cantadori, E., Brugnoli, M., Centola, M., Uffredi, E., Colonello, A., & Gullo, M. (2022). Date Fruits as Raw Material for Vinegar and Non-Alcoholic Fermented Beverages. In Foods 11(13) 1-13 MDPI. https://doi.org/10.3390/foods11131972

Cañete-Rodríguez, A. M., Santos-Dueñas, I. M., Jiménez-Hornero, J. E., Ehrenreich, A., Liebl, W., & García-García, I. (2016). Gluconic acid: Properties, production methods and applications—An excellent opportunity for agro-industrial by-products and waste bio-valorization. Process Biochemistry 51(12), 1891–1903. https://doi.org/10.1016/j.procbio.2016.08.028

Chandrasekaran, M., & Bahkali, A. H. (2013). Valorization of date palm (Phoenix dactylifera) fruit processing by-products and wastes using bioprocess technology - Review. Saudi Journal of Biological Sciences 20(2), 105–120. https://doi.org/10.1016/j.sjbs.2012.12.004

Dasa, Y. D. B., Putra, G. P. G., & Gunam, I. B. W. (2017). Karakteristik Cuka dari Cairan Pulpa Hasil Samping Fermentasi Biji Kakao (Theobroma cacao L.) pada Perlakuan Penambahan Inokulum Acetobacter aceti dan Lama Fermentasi. Jurnal Rekayasa dan Manajemen Agroindustri 5(2), 28–37. https://ojs.unud.ac.id/index.php/jtip/article/view/31582

Ernawaningtyas, E., & Yudhayanti, D. (2017). Penetapan kadar alkohol pada arak jowo yang beredar di ponorogo dengan metode destilasi. Jurnal EDUNursing, 1(2), 57-63. Retrieved fromhttps://journal.unipdu.ac.id/index.php/edunursing/article/view/1089

Hamden, Z., El-Ghoul, Y., Alminderej, F. M., Saleh, S. M., & Majdoub, H. (2022). High-quality bioethanol and vinegar production from Saudi Arabia dates: characterization and evaluation of their value and antioxidant efficiency. Antioxidants 11(6), 1155. https://doi.org/10.3390/antiox11061155

Hardoko, M., Prihanto, N. H., & Sasmito, B. B. (2019). Aktivitas Antioksidan dan Karakteristik Cuka Buah Mangrove dan Karakteristik Pedada (Sonneratia alba). JFMR-Journal of Fisheries and Marine Research 3(3), 322–330. https://doi.org/10.21776/ub.jfmr.2019.003.03.6

Hastiningsih, I. (2015). Krim Ekstrak Etanol Kulit Batang Pohon Nangka (Arthocarpus Heterophilus) Sama Efektifnya dengan Krim Hidrokuinon dalam Mencegah Peningkatan Jumlah Melanin pada Kulit Marmut (Cavia Porcelus) yang Dipapar Sinar UVB (Tesis). Denpasar: Universitas Udayana. ­ Retrieved fromhttps://adoc.pub/tesis-krim-ekstrak-etanol-kulit-batang-pohon-nangka.html

Hossain, S. J., Islam, M. R., Pervin, T., Iftekharuzzaman, M., Hamdi, O. A. A., Mubassara, S., Saifuzzaman, M., & Shilpi, J. A. (2017). Antibacterial, Anti-Diarrhoeal, Analgesic, Cytotoxic Activities, and GC-MS Profiling of Sonneratia apetala (Buch.-Ham.) Seed. Preventive Nutrition and Food Science 22(3), 157–165. https://doi.org/10.3746/pnf.2017.22.3.157

Hrichi, S., Cha, R., Alibrando, F., Altemimi, A. B., Babba, O., Oulad, Y., Majdoub, E., Nasri, H., Mondello, L., Babba, H., Mighri, Z., & Cacciola, F. (2022). Chemical Composition, Antifungal and Anti-Biofilm Activities of Volatile Fractions of Convolvulus althaeoides L. Roots from Tunisia. Molecules 27(20): 6834. https://doi.org/10.3390/molecules27206834

Hur, S. J., Lee, S. Y., Kim, Y. C., Choi, I., & Kim, G. B. (2014). Effect of fermentation on the antioxidant activity in plant-based foods. Food Chemistry 160, 346–356. https://doi.org/10.1016/j.foodchem.2014.03.112

Imamah, P. N., & Efendy, M. (2021). Analisis Cemaran Bakteri Escherichia coli pada Daging Ikan Pelagis (Studi Kasus) di Perairan Laut Utara dan Selatan Kabupaten Sampang. Juvenil:Jurnal Ilmiah Kelautan dan Perikanan 2(1), 17–24. https://doi.org/10.21107/juvenil.v2i1.9656

Iyabo Christianah, O. (2019). Alcoholic Beverages and Human Health: An Overview (1st ed). Frontiers and New Trends in the Science of Fermented Food and Beverages. https://doi.org/10.5772/intechopen.81054

La China, S., Zanichelli, G., De Vero, L., & Gullo, M. (2018). Oxidative fermentations and exopolysaccharides production by acetic acid bacteria: a mini review. Biotechnology Letters 40 (9–10), 1289–1302. https://doi.org/10.1007/s10529-018-2591-7

Matloob, M. H. (2014). Zahdi date vinegar: Production and characterization. American Journal of Food Technology 9(5), 231–245. https://doi.org/10.3923/ajft.2014.231.245

Mukhriani, M., Rusdi, M., Arsul, M. I., Sugiarna, R., & Farhan, N. (2019). Kadar Fenolik dan Flavonoid Total Ekstrak Etanol Daun Anggur (Vitis vinifera L). ad-Dawaa'Journal of Pharmaceutical Sciences 2(2), 95-102. https://doi.org/10.24252/djps.v2i2.11503

Nugrahani, H. N., Apriyani, I., & Bahri, S. (2021). Analisis Kadar Asam Asetat Hasil Fermentasi Buah Kedondong (Spondias dulcis Parkinson) dengan Metode Titrasi Alkalimetri. Sainstech Farma: Jurnal Ilmu Kefarmasian, 14(2), 97-101. https://doi.org/10.37277/sfj.v14i2.1013

Nurhayati, L. S., Yahdiyani, N., & Hidayatulloh, A. (2020). Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt dengan Metode Difusi Sumuran dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan 1(2), 41-46. https://doi.org/10.24198/jthp.v1i2.27537

Pagarra, H., & Anisa, N. (2022). Identifikasi Senyawa Kulit Buah Kakao Sulawesi 2 (Theobroma cacao L.) Menggunakan Etanol dan Aseton. Jurnal Sainsmat 11(1), 68–77. https://doi.org/10.35580/sainsmat111275162022

Park, K. M., Kim, H. J., Choi, J. Y., & Koo, M. (2021). Antimicrobial Effect of Acetic Acid, Sodium Hypochlorite, and Thermal Treatments against Psychrotolerant Bacillus cereus Group Isolated from Lettuce (Lactuca sativa L.). Foods 10(9), 1-13. https://doi.org/10.3390/foods10092165

Saeed, N. M., El-Demerdash, E., Abdel-Rahman, H. M., Algandaby, M. M., Al-Abbasi, F. A., & Abdel-Naim, A. B. (2012). Anti-inflammatory activity of methyl palmitate and ethyl palmitate in different experimental rat models. Toxicology and Applied Pharmacology 264(1), 84–93. https://doi.org/10.1016/j.taap.2012.07.020

Septiani, N. K. A., Parwata, I. M. O. A., & Putra, A. A. B. (2018). Penetuan Kadar Total Fenol, Kadar Total Flavanoid dan Skrining Fitokimia Ekstrak Etanol Daun Gaharu (Gyrinops versteegii). Jurnal Matematika dan Sains: Jurnal Matematika, Sains, dan Pembelajarannya 12(1), 78–89. Retrieved from https://ejournal.undiksha.ac.id/index.php/JPM/article/view/pril2018-7

Shaaban, M. T., Ghaly, M. F., & Fahmi, S. M. (2021). Antibacterial activities of hexadecanoic acid methyl ester and green-synthesized silver nanoparticles against multidrug-resistant bacteria. Journal of Basic Microbiology 61(6), 557–568. https://doi.org/10.1002/jobm.202100061

Younes, K. M., Romeilah, R. M., El-Beltagi, H. S., Moll, H. El, Rajendrasozhan, S., El-Shemy, H. A., & Shalaby, E. A. (2021). In-vitro evaluation of antioxidant and antiradical potential of successive extracts, semi-purified fractions and biosynthesized silver nanoparticles of Rumex vesicarius. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 49(1), 1–23. https://doi.org/10.15835/nbha49112293

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