Use of phosphorus- and potassium-solubilizing multifunctional microbes to support maize growth and yield

Ana Khalisha, Rahayu Widyastuti, Iswandi Anas Chaniago

Abstract

Intensive chemical fertilizer use has led to environmental problems, ecological impacts, and dependence on chemical fertilizers. Microbial inoculants (biofertilizers) combined with mineral fertilizers can be used to establish an environmentally friendly and sustainable agricultural practice. This study aimed to observe the effectiveness of multifunctional microbes (S. pasteuri and A. costaricaensis) in their wild-type and mutant forms. The microbes can simultaneously solubilize phosphorus and potassium from minerals (rock P and feldspar) to support maize growth and yield. Microbial viability in the zeolite carrier was tested, and the treatment was applied to the field to determine the effect on maize growth and yield. The results showed that zeolite could maintain the microbe population at an average of 108 CFU g-1 during 4 months of storage. A field test revealed that all microbes treatments combined with minerals without the addition of chemical fertilizers could support maize growth and yield by producing maize ear. In particular, mutant A. costaricaensis can support dry stalk weight and maize ear length as effective as chemical fertilizers due to its ability to increase available P and exchangeable K in the soil. Overall, microbes could provide P but not K from the minerals and soil for plant uptake.

Keywords

Nutrient uptake; Biofertilizer; Viability; Plant support; Maize ear

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References

Astriani, M., Zubaidah, S., Abadi, A. L., & Suarsini, E. (2020). Pseudomonas plecoglossicida as a novel bacterium for phosphate solubilizing and indole-3-acetic acid-producing from soybean rhizospheric soils of East Java, Indonesia. Biodiversitas Journal of Biological Diversity, 21(2), 578-586. https://doi.org/10.13057/biodiv/d210220

BPS. (2018). Area and Distribution of Critical Land by Province (Hectare), 2011-2018. Statistics Indonesia. https://www.bps.go.id/indicator/60/588/1/luas-dan-penyebaran-lahan-kritis-menurut-provinsi.html

Dandessa, C., & Bacha, K. (2018). Review on Role of phosphate solubilizing microorganisms in sustainable agriculture. International Journal of Current Research and Academic Review, 6(11), 48-55. https://doi.org/10.20546/ijcrar.2018.611.006

El-Ramady, H. R., Alshaal, T. A., Amer, M., Domokos-Szabolcsy, É., Elhawat, N., Prokisch, J., & Fári, M. (2014). Soil Quality and Plant Nutrition. In H. Ozier-Lafontaine & M. Lesueur-Jannoyer (Eds.), Sustainable Agriculture Reviews 14: Agroecology and Global Change (pp. 345-447). Springer International Publishing. https://doi.org/10.1007/978-3-319-06016-3_11

Etesami, H., Emami, S., & Alikhani, H. A. (2017). Potassium solubilizing bacteria (KSB):: Mechanisms, promotion of plant growth, and future prospects ­ A review. Journal of soil science and plant nutrition, 17, 897-911. http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162017000400005&nrm=iso

Eviati, & Sulaeman. (2009). Analisis Kimia Tanah, Air, Pupuk, dan Tanaman (B. H. Prasetyo, D. Santoso, & L. R. Widowati, Eds.). Indonesia Soil Research Institute (ISRI). https://balittanah.litbang.pertanian.go.id/ind/dokumentasi/juknis/juknis_kimia2.pdf

Flatian, A. N., Slamet, S., & Citraresmini, A. (2020). Pelarutan 3 Jenis Fosfat Alam oleh Fungi Pelarut Fosfat [Phosphate-solubilizing fungi; rock phosphate; pH; P released; fungi pelarut fosfat; fosfat alam; pH; pelarutan P]. Jurnal Tanah dan Iklim, 42(2), 83-90. http://ejurnal.litbang.pertanian.go.id/index.php/jti/article/view/8244

Gebreslassie, H. B. (2016). Effect of Potasium Fertilizer on Crop Production. Journal of Natural Sciences Research, 6(7), 81-86. https://iiste.org/Journals/index.php/JNSR/article/view/30184

Ginting, R. C. B., Saraswati, R., & Husen, E. (2006). Mikroorganisme Pelarut Fosfat. In R. D. M. Simanungkalit, D. A. Suriadikarta, R. Saraswati, D. Setyorini, & W. Hartatik (Eds.), Pupuk Organik dan Pupuk Hayati (pp. 141-158). Balai Besar Litbang Sumberdaya Lahan Pertanian, Badan Penelitian dan Pengembangan Pertanian, Kementerian Pertanian Indonesia.

Ingle, K. P., & Padole, D. A. (2017). Phosphate solubilizing microbes: An overview. International Journal of Current Microbiology and Applied Sciences, 6(1), 844-852. https://doi.org/10.20546/ijcmas.2017.601.099

Ishaq, L. F., Lukiwati, D., Benggu, Y. I., & Bako, P. O. (2021). Kajian jenis bahan pembawa dan lama simpan terhadap infektivitas dan efektivitas inokulan fungi mikoriza arbuskula. Jurnal Agrotek Tropika, 9(2), 177-188. https://doi.org/10.23960/jat.v9i2.4680

Kemenesdm. (2020). Neraca Sumber Daya Dan Cadangan Batubara Indonesia. Indonesia Ministry of Energy and Mineral Resources. http://psdg.geologi.esdm.go.id/index.php?option=com_content&view=article&id=1335&Itemid=610

Kementan. (2019). Keputusan Menteri Pertanian Nomor 261/KPTS/SR.310/M/4/2019 tentang Persyaratan Teknis Minimal Pupuk Organik, Pupuk Hayati, dan Pembenah Tanah. Ministry of Agriculture. http://simpel1.pertanian.go.id/api/dokumen/regulasi/dokumen-1579833905542.pdf

Klaic, R., Plotegher, F., Ribeiro, C., Zangirolami, T. C., & Farinas, C. S. (2017). A novel combined mechanical-biological approach to improve rock phosphate solubilization. International Journal of Mineral Processing, 161, 50-58. https://doi.org/10.1016/j.minpro.2017.02.009

Malusá, E., Sas-Paszt, L., & Ciesielska, J. (2012). Technologies for Beneficial Microorganisms Inocula Used as Biofertilizers. The Scientific World Journal, 2012, 491206. https://doi.org/10.1100/2012/491206

Meena, V. S., Maurya, B. R., & Verma, J. P. (2014). Does a rhizospheric microorganism enhance K+ availability in agricultural soils? Microbiological Research, 169(5), 337-347. https://doi.org/10.1016/j.micres.2013.09.003

Puspitawati, M. D., Sugiyanta, & Anas, I. (2014). Pemanfaatan Mikrob Pelarut Fosfat untuk Mengurangi Dosis Pupuk P Anorganik pada Padi Sawah. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 41(3). https://journal.ipb.ac.id/index.php/jurnalagronomi/article/view/8095

Roy, E. D., Willig, E., Richards, P. D., Martinelli, L. A., Vazquez, F. F., Pegorini, L., . . . Porder, S. (2017). Soil phosphorus sorption capacity after three decades of intensive fertilization in Mato Grosso, Brazil. Agriculture, Ecosystems & Environment, 249, 206-214. https://doi.org/doi.org/10.1016/j.agee.2017.08.004

Shiva, V. (1991). The Violence of the Green Revolution: Third World Agriculture, Ecology, and Politics. Zed Books. http://www.trabal.org/courses/pdf/greenrev.pdf

Stevenson, F. J., & Cole, M. A. (1986). Cycles of soil carbon, nitrogen, phosphorus, sulphur micronutrients, 2nd edition. Wiley.

Sukmadewi, D. K. T., Anas, I., Widyastuti, R., Anwar, S., & Citraresmini, A. (2021). The effectiveness of application of phosphorous and potassium solubilizing multifunctional microbes (Aspergillus costaricaensis and Staphylococcus pasteuri mutants) on maize growth. Journal of Degraded and Mining Lands Management, 8(2), 2681-2688. https://doi.org/10.15243/jdmlm.2021.082.2681

Viruel, E., Lucca, M. E., & Siñeriz, F. (2011). Plant growth promotion traits of phosphobacteria isolated from Puna, Argentina. Archives of Microbiology, 193(7), 489-496. https://doi.org/10.1007/s00203-011-0692-y

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