Soil carbon mineralization affected by hot water and ultrasound pretreatment

Toan Nguyen-Sy, Van Thanh Thi Do, Dong Pham Duy


Paddy soil has attracted several studies; however, the effects of pretreatment on soil carbon mineralization remain unclear. This study aimed at validating the effects of soil pretreatment by performing anaerobic incubation of 15 soil samples before treating at room temperature water boiling at 80°C or ultrasound assist at 37Hz and combining (hereafter are control, hot water, ultrasound, mixed hot water, and mixed ultrasound treatments) conducted with three replications. Results showed that initial extracted carbohydrate and incubation extracted carbohydrate (Ini-ECH and Incu-ECH) ranged from 211 to 691 mg kg−1 and 229 to 961 mg kg−1, respectively, and reached the highest values with hot water. control, ultrasound, and mixed ultrasound treatments showed the lowest Ini-ECH (211–269 mg kg−1), while the lowest Incu-ECH was linked to both mixed soil treatments with similar amounts (229–264 mg kg−1). Conversely, soil carbon mineralization (generated extracted carbohydrates during anaerobic incubation, Min-ECH) was similar in control, hot water, and ultrasound treatments (ranged from 271 to 393 mg kg−1) but tended to be a negative value in mixed soil treatments. Therefore, we conclude that hot water and ultrasound pretreatments do not increase soil carbohydrate potential but likely promote carbon decomposition.


Carbon mineralization; Extracted carbohydrate; Hot water extraction; Soil organic carbon; Ultrasound assist extraction

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Albalasmeh, A. A., Berhe, A. A., & Ghezzehei, T. A. (2013). A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry. Carbohydrate Polymers, 97(2), 253-261.

Albero, B., Tadeo, J. L., & Pérez, R. A. (2019). Ultrasound-assisted extraction of organic contaminants. TrAC Trends in Analytical Chemistry, 118, 739-750.

Bongiorno, G., Bünemann, E. K., Oguejiofor, C. U., Meier, J., Gort, G., Comans, R., Mäder, P., Brussaard, L., & de Goede, R. (2019). Sensitivity of labile carbon fractions to tillage and organic matter management and their potential as comprehensive soil quality indicators across pedoclimatic conditions in Europe. Ecological Indicators, 99, 38-50.

Chantigny, M. H., Harrison-Kirk, T., Curtin, D., & Beare, M. (2014). Temperature and duration of extraction affect the biochemical composition of soil water-extractable organic matter. Soil Biology and Biochemistry, 75, 161-166.

Cheng, W., Padre, A. T., Sato, C., Shiono, H., Hattori, S., Kajihara, A., Aoyama, M., Tawaraya, K., & Kumagai, K. (2016). Changes in the soil C and N contents, C decomposition and N mineralization potentials in a rice paddy after long-term application of inorganic fertilizers and organic matter. Soil Science and Plant Nutrition, 62(2), 212-219.

Cheng, W., Yagi, K., Akiyama, H., Nishimura, S., Sudo, S., Fumoto, T., Hasegawa, T., Hartley, A. E., & Megonigal, J. P. (2007). An empirical model of soil chemical properties that regulate methane production in Japanese rice paddy soils. J Environ Qual, 36(6), 1920-1925.

Davidson, E. A., & Janssens, I. A. (2006). Temperature sensitivity of soil carbon decomposition and feedbacks to climate change. Nature, 440(7081), 165-173.

DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, 28(3), 350-356.

Fischer, H., Meyer, A., Fischer, K., & Kuzyakov, Y. (2007). Carbohydrate and amino acid composition of dissolved organic matter leached from soil. Soil Biology and Biochemistry, 39(11), 2926-2935.

Gao, X., Huang, R., Li, J., Wang, C., Lan, T., Li, Q., Deng, O., Tao, Q., & Zeng, M. (2020). Temperature induces soil organic carbon mineralization in urban park green spaces, Chengdu, southwestern China: Effects of planting years and vegetation types. Urban Forestry & Urban Greening, 54, 126761.

Ghani, A., Dexter, M., & Perrott, K. W. (2003). Hot-water extractable carbon in soils: a sensitive measurement for determining impacts of fertilisation, grazing and cultivation. Soil Biology and Biochemistry, 35(9), 1231-1243.

Gunina, A., & Kuzyakov, Y. (2015). Sugars in soil and sweets for microorganisms: Review of origin, content, composition and fate. Soil Biology and Biochemistry, 90, 87-100.

Hamkalo, Z., & Bedernichek, T. (2014). Total, cold and hot water extractable organic carbon in soil profile: impact of land-use change. Zemdirbyste-Agriculture, 101(2), 125-132.

Kautsar, V., Cheng, W., Tawaraya, K., Yamada, S., Toriyama, K., & Kobayashi, K. (2020). Carbon and nitrogen stocks and their mineralization potentials are higher under organic than conventional farming practices in Japanese Andosols. Soil Science and Plant Nutrition, 66(1), 144-151.

Lee, Y. B., Lorenz, N., Dick, L. K., & Dick, R. P. (2007). Cold Storage and Pretreatment Incubation Effects on Soil Microbial Properties []. Soil Science Society of America Journal, 71(4), 1299-1305.

Li, L., Zhang, X., Zhang, P., Zheng, J., & Pan, G. (2007). Variation of organic carbon and nitrogen in aggregate size fractions of a paddy soil under fertilisation practices from Tai Lake Region, China. Journal of the Science of Food and Agriculture, 87(6), 1052-1058.

Liu, H., Wu, Y., Ai, Z., Zhang, J., Zhang, C., Xue, S., & Liu, G. (2019). Effects of the interaction between temperature and revegetation on the microbial degradation of soil dissolved organic matter (DOM) – A DOM incubation experiment. Geoderma, 337, 812-824.

Lowe, L. E. (1978). Chapter 2 Carbohydrates in Soil. In M. Schnitzer & S. U. Khan (Eds.), Developments in Soil Science (Vol. 8, pp. 65-93). Elsevier.

Nguyen-Sy, T., Cheng, W., Kimani, S. M., Shiono, H., Sugawara, R., Tawaraya, K., Watanabe, T., & Kumagai, K. (2020). Stable carbon isotope ratios of water-extractable organic carbon affected by application of rice straw and rice straw compost during a long-term rice experiment in Yamagata, Japan. Soil Science and Plant Nutrition, 66(1), 125-132.

Nguyen-Sy, T., Nguyen-Thi, T., Tran-Thi, N. T., Do-Thi, V. T., Hanh, D. H., Le-Thi, D. H., Nguyen-Thi, T. C., Nguyen-Hoang, P. S., & Le-Duc, T. (2021, 12-13 March 2021). Ultrasound as a green technique to enhance soil mineralization potential. Proceeding of 2020 Applying New Technology in Green Buildings (ATiGB), Da Nang City, Viet Nam.

Nguyen-Sy, T., Tan, X., Phuong, N. T. D., Aron, N. S. M., Chew, K. W., Khoo, K. S., Thu, T. T. N., Thi Lim, D., Dong, P. D., Ang, W. L., & Show, P. L. (2021). Advanced green bioprocess of soil carbohydrate extraction from long-term conversion of forest soil to paddy field. Journal of Environmental Chemical Engineering, 9(5), 106021.

Pansu, M., & Thuriès, L. (2003). Kinetics of C and N mineralization, N immobilization and N volatilization of organic inputs in soil. Soil Biology and Biochemistry, 35(1), 37-48.

Rakhsh, F., & Golchin, A. (2018). Carbohydrate concentrations and enzyme activities as influenced by exchangeable cations, mineralogy and clay content. Applied Clay Science, 163, 214-226.

Rendana, M., Idris, W. M. R., Abdul Rahim, S., Ali Rahman, Z., & Lihan, T. (2021). Characterization of physical, chemical and microstructure properties in the soft clay soil of the paddy field area. Sains Tanah - Journal of Soil Science and Agroclimatology, 18(1), 81-88.

Sardiana, I. K., & Kusmiyarti, T. B. (2021). Sustainability performance of organic farming at vegetable fields in Tabanan, Bali, Indonesia. Sains Tanah - Journal of Soil Science and Agroclimatology, 18(1), 7-14.

Tanaka, H., Hamada, R., Kondoh, A., & Sakagami, K.-i. (1990). Determination of Component Sugars in Soil Organic Matter by HPLC. Zentralblatt für Mikrobiologie, 145(8), 621-628.

Tang, S., Cheng, W., Hu, R., Guigue, J., Hattori, S., Tawaraya, K., Tokida, T., Fukuoka, M., Yoshimoto, M., Sakai, H., Usui, Y., Xu, X., & Hasegawa, T. (2021). Five-year soil warming changes soil C and N dynamics in a single rice paddy field in Japan. Science of The Total Environment, 756, 143845.

Tang, S., Cheng, W., Hu, R., Guigue, J., Kimani, S. M., Tawaraya, K., & Xu, X. (2016). Simulating the effects of soil temperature and moisture in the off-rice season on rice straw decomposition and subsequent CH4 production during the growth season in a paddy soil. Biology and Fertility of Soils, 52(5), 739-748.

Tang, S., Cheng, W., Hu, R., Nakajima, M., Guigue, J., Kimani, S. M., Sato, S., Tawaraya, K., & Xu, X. (2017). Decomposition of soil organic carbon influenced by soil temperature and moisture in Andisol and Inceptisol paddy soils in a cold temperate region of Japan. Journal of Soils and Sediments, 17(7), 1843-1851.

Uzoho, B. U., & Igbojionu, G. U. (2014). Carbohydrate Distribution of Particle Size Fractions of Soils in Relation to Land-use Types in Mbaise, Southeastern Nigeria. Journal of Biology, Agriculture and Healthcare, 4, 27-36.

Walkley, A. (1947). A critical examination of a rapid method for determining organic carbon in soils-effect of variations in digestion conditions and of inorganic soil constituents. Soil Science, 63(4), 251-264.

Wang, W., Wu, X., Chen, A., Xie, X., Wang, Y., & Yin, C. (2016). Mitigating effects of ex situ application of rice straw on CH4 and N2O emissions from paddy-upland coexisting system. Scientific Reports, 6(1), 37402.

Wu, X., Nguyen-Sy, T., Sun, Z., Wantanabe, T., Tawaraya, K., Hu, R., & Cheng, W. (2020). Soil Organic Matter Dynamics as Affected by Land Use Change from Rice Paddy to Wetland. Wetlands, 40(6), 2199-2207.


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