Abiotic Stress and Biofertilizer on The Pereskia bleo (Kunth) DC. Against Growth, Proline, and Antioxidants

Intan Christin Dullah, Sulandjari Sulandjari, Supriyono Supriyono

Abstract

Pereskia bleo is a spiny bush from the genus Pereskia and family Cactaceae. Its leaves are shiny green, flowering, and fruitful. Some researchers to indicate that P.bleo leaves contain high antioxidants, anti-cancer, and are traditionally used for diabetic, hemorrhoids, hypertension, and gastric pain. The aim of this study was to determine the effect of salinity stress and water volume as well as the role of biofertilizer on the growth of Pereskia bleo and antioxidant content. Using Nested design (completely randomized factorial) with NaCl treatment; 0 ppm, 5 ppm, 10 ppm, and biofertilizer; 5 ppm, 10 ppm, each nested in a water volume of 500 ml, 300 ml and 150 ml per polybag. Each treatment was repeated three times, resulting in a total sample of 54 plants. Meanwhile, the findings indicate that abiotic pressure suppresses growth and proline, but not on antioxidants. There is an interaction between salinity and biofertilizer against antioxidants. Abiotic Stress can increase the antioxidant content.

Keywords

Biofertilizer; Drought stress; Pereskia bleo; Salinity; Secondary metabolites

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References

Abriz, S.F & Torabian, S. 2017. Antioxidant enzyme and osmotic adjustment changes in bean seedlings as affected by biochar under salt stress. Ecotoxicology and Environmental Safety 137 (2017) 64–70. DOI: 10.1016/j.ecoenv.2016.11.029

Agati, G., Azzarello, E.,Pollastri, S., dan Tattini, M. 2012. Review Flavonoids as antioxidants in plants: Location and functional significance. Plant Science 196 (2012) 67– 76. DOI: 10.1016/j.plantsci.2012.07.014

Ai, S.N dan Banyo, Y. 2011. Kosentrasi Klorofil Daun Sebagai Indikator Kekurangan Air Pada Tanaman. Jurnal Ilmiah Sains Vol. 11 No. 2. DOI: https://doi.org/10.1234/jis.v11i2.202

Ashraf M, Orooj A. 2006. Salt stress effects on growth, ion accumulation and seed oil concentration in anarid zone traditional medicinal plant ajwain (Trachyspermum ammi [L.] Sprague). J Arid Environ 64(2):209-20. DOI: 10.4236/ajps.2017.88121

Aziz,E.E.,Al-Amier,H.,andCraker,L. E. 2008.Influenceofsaltstresson growthandessentialoilproduction in peppermint,pennyroyal,andapple mint. J. HerbsSpicesMed.Plants 14, 77–87. https://doi.org/10.1080/10496470802341375

Bargmann, B.O.R., A.M. Laxalt, B. ter Riet, B. van Schooten, E. Merquiol, C. Testerink, M.A. Haring, D. Bartels, and T. Munnik. 2009. Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants. Plant and Cell Physiology 50 (1) : 78 - 89. doi: 10.1093/pcp/pcn173. Epub 2008 Nov 18.

Bhardwaj, D., Ansari, M.W., Sahoo, R.K. and Tuteja, N. 2014. Biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity. Microbial Cell Factories. 13: 66. doi: 10.1186/1475-2859-13-66.

Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies.Plant Soil 39: 205-207. DOI https://doi.org/10.1007/BF00018060

Hussain K, Majeed A, Nawaz Kh, Khizar HB, Nisar MF. 2009. Effect of different levels of salinity on growth and ion contents of black seeds (Nigella sativa L.).. Curr Res J Biol Sci . 1(3):135-8. http://maxwellsci.com/print/crjbs/(3)135-138.pdf

Hong,N dan Nor,H.I.2012.Extration and characterization of mucilage from leaves of Pereskia bleo (rose cactus).Jurnal teknol dan industri pangan,vol.XXXIII no.2 th.2012. DOI: http://dx.doi.org/10.6066/jtip.2012.23.2.210

Li, J., Yaling, W., Juanjuan, W., Yu, P., Chenggang, S., & Xiangguo, Z., 2018. A tomato proline-, lysine-, and glutamic-rich type gene SpPKE1 positively regulates drought stress tolerance. Biochemical and Biophysical Research Communications (2018). https://doi.org/10.1016/j.bbrc.2018.03.222.

Liu, H., X, Wang., D, Wang., Z, Zou., dan Z. Lang. 2010. Effect of drought stress on growth and accumulation of active constituents in Salvia miltiorrhiza Bunge. Industrial Crops and Products. 33 (2011) 84–88. DOI 10.1016/j.indcrop.2010.09.006

Jamil M, Rehman S, Lee KJ, Kim JM, Kim HS, Rha ES. 2007.Salinity reduced growth PS II photochemistry and chlorophyll content in radish. Sci Agric; 64: 1-10. http://dx.doi.org/10.1590/S0103-90162007000200002.

Jaleel CA, Sankar B, Sridharan R. Panneerselvam R. 2008. Soil salinity alters growth, chlorophyll content, and secondary metabolite accumulation in Catharanthus roseus. Turk J Biol . 32:79-83. https://pdfs.semanticscholar.org/654d/f82d1a60fa4486f4da3d6b93ca0ca7d80fdb.pdf

Kurnia, Theresa Dwi., Suprihati. 2013. Proline Sebagai Penanda Ketahanan Kekeringan Dan Salinitas Pada Gandum. Conference Paper.https://www.researchgate.net/publication/303971617

Mathius, N.T., T. Liwang, M.I. Danuwikarsa, G. Suryatmana, H. Djajasukanta, D. Saodah, dan I.G.P.W. Astika. 2004. Respon Biokimia Beberapa Progeni Kelapa Sawit (Elaeis guineensis Jacq.) terhadap Cekaman Kekeringan pada Kondisi Lapang. Menara Perkebunan 72 (2) : 38 - 56. http://mp.iribb.org/index.php/mpjurnal/article/viewFile/121/pdf

Murillo, E.A. J.Mel´endez-Mart´ınez, and F. Portugal, 2010. “Screening of vegetables and fruits from Panama for rich sources of lutein and zeaxanthin,” Food Chemistry, vol. 122, no. 1, pp. 167–172. DOI : 10.1016/j.foodchem.2010.02.034

Molyneux P 2004 The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. J. Sci. Technol 26 (2) 211-219. https://www.semanticscholar.org/paper/The-use-of-the-stable-free-radical-(-DPPH-)-for-Molyneux/24d63a7e2670f38d6cfd72242295061be53b1812

Reddy AR, Chiatanya KV & Vivekanandan M. 2004. Draught induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161: 1189-1202. https://doi.org/10.1016/j.jplph.2004.01.013

Munns, R. and M. Tester.2008. “Mechanisms of salinity tolerance,” Annual Review of Plant Biology, vol. 59, pp. 651–681. Sogand Zareisedehizadeh,1 Chay-Hoon Tan,2 and Hwee-Ling Koh1. 2014. A Review of Botanical Characteristics, Traditional Usage, Chemical Components, Pharmacological Activities and Safety of Pereskia bleo (Kunth) DC. Evidence-Based Complementary and Alternative Medicine, Article ID 326107, 11 pages http://dx.doi.org/10.1155/2014/326107

Malek, S. N. A., S. K. Shin, N. A.Wahab, and H. Yaacob, “Cytotoxic components of Pereskia bleo (Kunth) DC. (Cactaceae leaves,” Molecules, vol. 14, no. 5, pp. 1713–1724, 2009. DOI: 10.1155/2011/368692

Tabatabaie SJ, Nazari J. 2007. Influence of nutrient concentration and NaCl salinity on growth, photosynthesis and essential oil content of peppermint and lemon verbena. Turk J Agric; 31:245-53. http://dergipark.gov.tr/tbtkagriculture/issue/11614/138394

Taïbi ,K., Fadhila. T., Leila A. A., Amel, E., Moulay,B dan José, M. 2016. Effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidant defence systems in Phaseolus vulgaris L. South African Journal of Botany 105 (2016) 306–312. DOI: 10.1016/j.sajb.2016.03.011

Tan,M.L.;S.F.Sulaiman, N. Najmuddin,M.R. Samian and T.S.Tengku Muhamad,2004. Methanolic extract of Pereskia bleo (Kunth)DC. (Cactaceae) induces apoptosis in breast carcinoma, T47-D cell line. J.Ethnopharmacol.,96.287-294. DOI: 10.1016/j.jep.2004.09.025

Wahab, A.R., C. C. Guilhon, P. D. Fernandes, and F. Boylan, 2012. “Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts,” Journal of Ethnopharmacology, vol. 144, no. 3, pp. 741–746. https://doi.org/10.1016/j.jep.2012.10.029

Wiart C. 2006. Medicinal plants of the Asia-Pasifik: drugs for the future. Singapore: World Scientific. https://doi.org/10.1142/5834

Winter, K., Milton, G dan Joseph A. M. H. 2011. Drought-stress-induced up-regulation of CAM in seedlings of a tropical cactus, Opuntia elatior, operating predominantly in the C3 mode. Journal of Experimental Botany, Vol. 62, No. 11, pp. 4037–4042, 2011. doi: 10.1093/jxb/err106. Epub 2011 Apr 19.

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