Cryopreservation of Simmental cattle semen with egg yolk from different avian species and level glycerol of different in tris diluent

Yendraliza Yendraliza, Yudi Gusti Rahman


Objective: This research was conducted to determine the effect of egg yolk from different avian species and the level glycerol of different in maintaining sperm quality in Simmental cattle in tris diluent.

Methods: Semen comes from Simmental (n=3) kept at the Tuah Sakato Artificial Insemination Center (BIB), Payakumbuh, West Sumatra. The diluent used in this research was tris egg yolk derived from three types of egg yolk (Factor A; the egg yolks used to come from ducks, quails and chickens) and different doses of glycerol (Factor B; 6, 6.5 and 7%). The research method used was a Randomized Block Design (RBD) with 2 factors. The shelter sperm is a group. The variables observed were the percentage of motility, abnormality, percentage of live sperm and intact plasma membrane of Simmental cattle sperm after thawing.

Results: The results showed no interaction between glycerol levels with types of egg yolk in tris extender of Simmental sperm quality. The tris diluent with duck egg yolk was better than chicken egg yolk which was equivalent which was able to motility up to 52.67%, percentage of live sperm was 70.78, abnormal sperm was only 14.44% and IPM was up to 42.44%. The dose of glycerol 6 ml is better than 6.5 ml and 7 ml resulting in motility up to 52.44%, live percentage reaching 70.22%, abnormal sperm at 14.33% and IPM up to 43%.

Conclusion: The use of duck egg yolk in tris extender and the addition of 6% glycerol resulted in better sperm quality in Simmental cattle with high motility, percentage of live sperm, IPM and lower abnormal sperm.


Abnormalities; Motility; Percentage live; Plasma membrane intact

Full Text:



  1. Ducha, N., T. Susilawati, Aulanni'am, and S. Wahyuningsih. 2013. Spermatozoa motility and viability of Limousin Cattle during refrigerator storage in CEP-2 diluent with egg yolk supplementation. J. Doctor. Animal. 7(1): 5–8.
  2. Magistri, P. M, R. Yaswir, and Y. Alioes. 2016. Effect of giving various processed eggs on total blood cholesterol of mice. J. Andalas Health. 5(3): 534-539.
  3. Polat, S. E., B. O. Citil, and M. Garip. 2013. Fatty acid composition of the yolk of nine poultry species kept in their natural environment. Anim. Science Papers and Reports. 31(4): 363­­-369.
  4. Best, B. P. 2015. Cryoprotectant toxicity: facts, issues, and questions. Rejuvenation Res. 18(5):422–436. Doi: 10.1089/rej.2014. 1656
  5. Intellect, E., A. Kocyigit, and M. Selcuk. 2014. Role of low density lipoprotein in semen preservation. J. Vet. Kocatepe. 7(1): 69-74. Doi: 10.5578/kvj.7429
  6. Setiono, N., S. Suharyati, and P. E. Santosa. 2015. Quality of frozen Brahman cow smen with different doses of glycerol cryopectant in egg yolk tris citrate diluent. J. Ilm. breeder Integrated. 3(2): 61–69.
  7. Ariantie, O. S., T. L. Yusuf, D. Sajuthi, and R. I. Arifiantini. 2013. Effect of glycerol and dimethylformamide (DMF) cryoprotectants on buck Etawah Crossbreed frozen semen using modified tris diluents. J. Anim. Sci. Vet. 18(4): 239–250. Doi: 10.14334/jitv.v18i4.327
  8. Yendraliza, Y., M. Musyrifin, E. Elviriadi, Z. Zumarni, and M. Rodiallah. 2019. Spermatozoa viability of Bali cattle using andromed diluent with the addition of different carrot extract concentrations. J. Sci. Technol. breeder Trop. 6(2): 239–245. Doi: 10.33772/jitro.v6i2.5936
  9. Hirwa, C. D., D. R. Kugonza, A. Kayitesi, T. Murekesi, F. Semahoro, G. Uwimana, and R. Habimana. 2019. Phenotypes, production systems and reproductive performance of indigenous chickens in contemporary Rwanda. Int. J. Livest. Prod. 10(10):213–231. Doi: 10.5897/ijlp2019.0618
  10. 10. Engidawork, B. 2018. Artificial Insemination service efficiency and constraints of artificial insemination service in selected districts of Harari National Regional State, Ethiopia. Open J. Anim. sci. 08(03):239–251. Doi: 10.4236/ojas.2018.83018
  11. Goshme, S., T. Asfaw, C. Demiss, and S. Besufekad. 2021. Evaluation of motility and morphology of frozen bull semen under different thawing methods used for artificial insemination in North Shewa zone, Ethiopia. Heliyon. 7(10): e08183. Doi: 10.1016/j.heliyon.2021.e08183
  12. Morrell, J. M., A. S. Valeanu, N. Lundeheim, and A. Johannisson. 2018. Sperm quality in frozen beef and dairy bull semen. Acta Vet. scand. 60(1):1–10. Doi: 10.1186/s13028-018-0396-2
  13. Santoso, S., H. Herdis, R. I. Arifiantini, A. Gunawan, and C. Sumantri. 2021. Characteristics and potential production of frozen semen of Pasundan Bull. Trop. Anim. Sci. J. 44(1):24–31. Doi: 10.5398/tasj.2021.44.1.24
  14. Sieme, H., H. Oldenhof, and W. F. Wolkers. 2016. Mode of action of cryoprotectants for sperm preservation. Anim. Reprod. Sci. 169:2–5. Doi: 10.1016/J.ANIREPROSCI.2016.02.004
  15. Perumal, P., S. K. Srivastava, S. K. Ghosh, K. K. Baruah, M. H. Khan, and J. S. Rajoriya. 2016. Effect of low density lipoprotein on replacement of egg yolk in liquid preservation of mithun semen. Indian J. Anim. Sci. 86(4): 55–57.
  16. Akhter, S., B. A. Rakha, M. S. Ansari, A. U. Husna, S. Iqbal, and M. Khalid. 2017. Evaluation of quail and turkey egg yolk for cryopreservation of Nili-Ravi buffalo bull cement. theriogenology. 87. Doi: 10.1016/j.theriogenology.2016.09.002
  17. SNI (Indonesian National Standard). 2017. Frozen semen - Part 1: Bovine cattle in the Indonesian National Standard Agency, Jakarta: Indonesia. pp. 1–4.
  18. Husna, A. U., R. Ejaz, S. Qadeer, A. Azam, BA Rakha, MS Ansari, Q. Shahzad, M. Javed, MH Vazquez-Levin, and S. Akhter, S. 2016. A comparative analysis of sperm selection procedures prior to cryopreservation for Nili-Ravi buffalo bull (Bubalus bubalis) semen-: Assessment of its impact on post-thaw sperm functional quality. Anim. Reprod. Sci. 174:29–36. Doi: 10.1016/j.anireprosci.2016.08.015
  19. García, W., A. Tabarez, and M. J. Palomo. 2017. Effect of the type of egg yolk, removal of seminal plasma and donor age on ram sperm cryopreservation. Anim. Reprod. 14(4): 1124–1132. Doi: 10.21451/1984-3143-AR916
  20. Herbowo, M. T., R. I. Arifiantini, N. W. K. Karja, and R. G. Sianturi. 2019. Cryopreservation of swamp buffalo semen in skim milk yolk-based diluent with two different cryoprotectants. Trop. Anim. sci. J. 42(1):13–18. Doi: 10.5398/tasj.2019.42.1.13


  • There are currently no refbacks.