Toksisitas Ekstrak Biji Mahoni Terhadap Ulat Plutella xylostella pada Daun Kubis

Ayu Putri Dwi Ariyanti, Subagiya Subagiya, Ato Sulistyo

Abstract

Plutella xylostella is one of cabbage’s main pests. Botanical insecticides as alternative of chemical insecticides need to be expand, one of them is mahogany seed extract. Mahogany seed exctract contains saponin and flavonoid as reported mortal to P. xylostella. This study aimed to know the effective concentration of mahogany seed extract to manage P. xylostella different larva instar and to know LC50 value of mahogany seed extract to P. xylostella. This method used was completely randomized experimental design (CRD). Two factors used were mahogany seed exctract concentrations (0 gL-1, 5 gL-1, 10 gL-1, 20 gL-1, and 40 gL-1) and larva instar (first and third instar). Each treatment repeated four times. The results showed that percentage of larva mortality first instar higher than third instar. Concentration 20 gL-1 of mahogany seed exctract effective to kill first instar larva, and concentration 40 gL-1 effective for third instar. Percentage of pupa mortality highest in concentration 10 gL-1 for first instar and concentration 40 gL-1 for third instar larva. Antifeedant increased as concentration given, while the eating ability that higher in third instar was decreased. LC50 for first instar larva was 2,6 gL-1 and third instar was 13,7 gL-1, meaned that mahogany seed extract more toxic to first instar than third instar larva.

Keywords

botanical insecticide, lethal concentration, mortality

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References

Agazali, F., Hoesain, M., & Prastowo, S. (2015). Efektivitas insektisida nabati daun tanjung dan daun pepaya terhadap mortalitas ulat grayak

(Spodoptera litura F.). Berkala Ilmiah Pertanian 1(1):1-5.

Ariyanti, R., Yenie, E., & Elystia, S. (2017). Pembuatan pestisida nabati dengan cara ekstraksi daun pepaya dan belimbing wuluh. Jom Fteknik 4(2):1-9.

Begna, F., & Damtew, T. (2016). The effect of plant extract for managing diamondback moth, Plutella xylostella L. (Lepidoptera : Plutellidae) on head cabbage in Ethiopia. J of Biology, Agriculture and Healthcare 6(5):72-80. DOI:10.13140/RG.2.2.35870.84803.

Budianto, F., & Tukiran. (2012). Bioinsektisida dari tumbuhan bakau merah (Rhizhopora stylosa. Griff) (Rhizophoraceae). UNESA J of Chemistry 1(1):19-25.

Dono, D., Ismayana, S., Idar, Prijono, D., & Muslikha, I. (2010). Status dan mekanisme resistensi biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap insektisida organofosfat serta kepekaannya terhadap insektisida botani ekstrak biji Barringtonia asiatica. J Entomol. Indon. 7(1):9-27.

Dono, D., Natawigena, W. D., & Majid, M, G. (2012). Bioactivity of methanolic seed extract of Barringtonia asiatica L. (Kurz) (Lecythidaceae) on biological characters of Spodoptera litura (Fabricius) (Lepidoptera : Noctuidae). International Research J of Agricultural Science and Soil Science 2(11): 469-475.

Dono, D., & Susanerwinur. (2013). Toksisitas dan anti oviposisi ekstrak metanol kulit jambu mete (Anacardium occidentale L.) (Anacardiaceae) terhadap Crocidolomia pavonana F. (Lepidoptera : Pyralidae). Bionatura J Ilmu-ilmu Hayati dan Fisik 15(2):79-82.

Ginting, M. S., Pelealu, J., & Pinaria, B. A. N. (2017). Efektivitas beberapa insektisida nabati terhadap hama Plutella xylostella Linn. (Lepidoptera; Plutellidae) pada Tanaman Kubis (Brassica oleracea L.) di Kabupaten Minahasa. Agri-Sosio Ekonomi Unstrat 13(3A):295-302.

Gowri, G., & Manimegalai, K. (2016). Biology of diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) of cauliflower under laboratory condition. International J of Fauna and Biological Studies 3(5):29-31.

Hasnah, H. (2009). Efektivitas ekstrak buah mengkudu (Morinda citrifolia L.) terhadap mortalitas Plutella xylostella L. pada tanaman sawi. J Floratek 4:29-40.

Hermawan, W., Erawan, E. S., & Hardiansyah, C. (2010). Efek antifidan Andrografolida terhadap aktivitas kelenjar pencernaan larva Plutella xylostella L. Bionatura J Ilmu-ilmu Hayati dan Fisik 12(1):50-56.

Herminanto. (2006). Pengendalian hama kubis Crocidolomia pavonana F. menggunakan ekstrak kulit buah jeruk. J Pembangunan Pedesaan 6(3):165-174.

Heviyanti, M., Husni, & Rusdy, A. (2016). Efektifitas ekstrak biji mahoni (Swietenia mahogani Jacq.) terhadap mortalitas dan rata-rata waktu kematian larva Plutella xylostella pada tanaman sawi. J Agrosamudra 3(1):27-38.

Javier, A. M. V., Ocampo, V. R., Ceballo, F.A., & Javier, P. A. (2016a). Insecticidal activity of four essential oils against diamondback moth, Plutella xylostella Linnaeus (Lepidoptera: Pyralidae). The Philippine Agricultural Scientist 99(2):156-163.

Javier, A. M. V., Ocampo, V. R., Ceballo, F. A., & Javier, P. A. (2016b). Insecticidal activity of crude ethanolic extracts of selected Philippine plants against diamondback moth, Plutella xylostella. Philippine J of Science 148(1):33-43.

Khaidir, & Hendrival. (2013). Pengujian penghambatan aktivitas makan dari ekstrak daun Lantana camara L. (Verbenaceae) terhadap larva Plutella xylostella L. (Lepidoptera : Ypnomeutidae). J Floratek 8:35-44.

Lina, M. (2016). Pengaruh pemberian ekstrak daun legundi (Vitex trifolia) sebagai pestisida nabati pengendalian hama Plutella xylotella pada tanaman sawi (Brasscia juncea). J Biologi 5(4):34-40.

Rahmawati, S., Karimuna, S. R., & Yasnani. (2018). Efektivitas lilin aromatik dari ekstrak kulit jeruk nipis (Citrus aurantifolia) sebagai insektisida alami terhadap lalat rumah (Musca domestica). J Ilmiah Kesehatan Masyarakat 3(3):1-8.

Rusandi, R., Mardhiansyah, M., & Arlita, T. (2016). Pemanfaatan ekstrak biji mahoni sebagai pestisida nabati untuk mengendalikan hama ulat grayak (Spodoptera litura F) pada pembibitan

Acacia crassicarpa A. Cunn. ex Benth. Jom Faperta UR (3)1:1-6.

Sapindal, E., Ong, K. H., & King, P. J. H. (2018). Efficacy of Azadirachta excelsa vinegar against Plutella xylostella. International J of Pest Management 64(1):39-44. DOI:10.1080/09670874.2017.1293866.

Sari, M., Lubis, L., & Pangestiningsih, Y. (2013). Uji efektivitas beberapa insektisida nabati untuk mengendalikan ulat grayak (Spodoptera litura F.) (Lepidoptera : Noctuidae) di laboratorium. J Online Agroteknologi 1(3):560-569.

Sariri, A. K., Mulyono, M. W., & Tari, A. I. N. (2018). The utilization of microbes as a fermentation agent to reduce saponin in Trembesi leaves (Sammanea saman). IOP Conference Series : Earth and Enviromental Science 142:1-5. DOI:10.1088/1755-1315/142/1/012041.

Suryani, A. I., Hariani, N., Majid, A. F., & Risqa, S. (2017). Persentase mortalias ulat grayak terhadap pemberian eksrak daun bunga pukul empat. Bionature 18(2):118-122.

Susanto, W. (2013). Pengaruh aplikasi ekstrak biji mahoni (Swietenia mahagoni Jacq.) terhadap mortalitas ulat kubis (Plutella xylostella L.) (Lepidoptera : Plutellidae). Skripsi. Universitas Lampung, Lampung.

Tanor, M. N. (2014). Methanol extract of Barringtonia asiatica, Kurz. quite effective induce mortality of Spodopthera litura, Fabr. larvae on soybean plants. J of Biology and Life Science 5(2):159-164. DOI:10.5296/jbls.v5i2.5587.

Tekeli, A., Celik, L., & Kutlu, H. R. (2007). Plant extracts; a new rumen moderator in ruminant diets. J of Tekirdag Agricultural Faculty 4(1):71-79.

Toana, M. H. (2007). Pengaruh konsentrasi ekstrak kulit jeruk nipis (Citrus aurantifolia S.) terhadap kepadatan populasi dan intensitas serangan

Plutella xylostella L. (Lepidoptera : Plutellidae) pada tanaman kubis. Agroland 14(3):195-200.

Utami, I. W., & Cahyati, W. H. (2017). Potensi ekstrak daun kamboja sebagai insektisida terhadap nyamuk Aedes aegypti. Higeia 1(1):22-28.

Wina, E. (2012). The use of plant bioactive compounds to mitigate enteric methane in ruminants and its application in Indonesia. J Wartazoa 22:24-34.

DOI: https://doi.org/10.20961/agsjpa.v22i1.28600