ISOLASI, SELEKSI DAN OPTIMASI BAKTERI PENGHASIL FITASE DARI TANAH VULKANIK DI GUCI, TEGAL
Abstract
The purpose of this study was to isolated, selected and characterized phytase bacteria derived from volcanic soil in the Guci, Tegal. Bacteria from the volcanic soil was isolated and selected on Luria Betany (LB) media containing 4% phytic acid. Selected colonies were optimized by physical character with determining the relative activity. Colonies of bacteria could be isolated from the volcanic soil, selected, and had the highest relative activity in colonies of TG3 and TG3-5. TG3-5 bacterial colonies producing phytate had the highest optimum value at a temperature of 55°C, pH 7, the incubation time of 90 minutes, the substrate concentration of 5% and a best metal cofactor on Zn2+ (10-4M). Volcanic soil in the Guci, Tegal containing phytase-producing bacteria with certain physical characteristics.A
Keywords
Full Text:
PDFReferences
Acuna, J. J., M. A. Jorquera, O. A. Martínez, D. Menezes-Blackburn, M. T. Fernandez, P. Marschner, R. Greiner and M. L. Mora1. 2011. Indole acetic acid and phytase activity produced by rhizosphere bacilli as affected by pH and metals. Journal of Soil Science and Plant Nutrition. 11(3):1-12.
Gupta, R. K., S. S. Gangoliya, and N. K. Singh. 2015. Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains. Journal of food science and technology. 52(2): 676-684.
Jorquera, M. A., M. T. Hernández, Z. Rengel, P. Marschner and M. de la L. Mora. 2008. Isolation of culturable phosphobacteria with both phytate-mineralization and phosphate-solubilization activity from the rhizosphere of plants grown in a volcanic soil. Biology and Fertility of Soils. 44:1025–1034.
Jorquera, M. A., D. E. Crowley, P. Marschner, R. Greiner, M. T. Fernandez, D. Romero, D. M. Blackburn and M. de la L. Mora. 2011. Identi¢cation of b-propeller phytase-encoding genes in culturable Paenibacillus andBacillus spp. fromthe rhizosphere of pasture plants onvolcanic soils. Federation of European Microbiological Societies Microbiology Ecology. 75(2011):163-172.
Kanpiengjai, A., K. Unban, R. Prathanaphon and C. Khanongnuch. 2013. Optimal medium and conditions for phytase production by thermophilic bacterium, Anoxybacillus sp. MHW14. Food and Applied Bioscience Journal. 1(3): 172-189.
Kumar, V., P. Singh, M. A. Jorquera, P. Sangwan, P. Kumar, A. K. Verma and S. Agrawal. 2013. Isolation of phytase-producing bacteria from Himalayan soils and their effect on growth and phosphorus uptake of Indian mustard (Brassica juncea). World Journal Microbiology and Biotechnology. 29(2013):1361-1369.
Liu, S. Y., R. M. Boldb, P. W. Plumsteadb, P. H. Selle. 2015. Effects of 500 and 1000FTU/kg phytase supplementation of maize-based diets with two tiers of nutrient specifications on performance of broiler chickens. Animal Feed Science and Technology. 207(2015):159-167.
Manobhavan, M., A. V. Elangovan, M. Sridhar, D. Shet, S. Ajith, D. T. Pal and N. K. S. Gowda. 2016. Effect of super dosing of phytase on growth performance, ileal digestibility and bone characteristics in broilers fed corn–soya‐based diets. Journal of Animal Physiology and Animal Nutrition. 100(1): 93-100.
Mehta, R., P. Singhal, H. Singh, D. Damle and A. K. Sharma. 2016. Insight into thermophiles and their wide-spectrum applications. Biotechnology. 6:81(1-9).
Nuhriawangsa, A. M. P., Z. Bachruddin, Sajidan, and A. Wibowo, 2011. Pengaruh Pemberian Fitase dari Bakteri Rekombinan pEAS1-AMP Asli Indonesia terhadap Performan Produksi dan Profil Darah pada Ayam Broiler Fase Grower. In: Proceeding of National Seminar on Zootechniques for Indogenous Resources Development. Faculty of Animal Agriculture Diponegoro University, JPPT and Indonesian Society of Animal Agriculture, Semarang. ISAA Publication. 1(2012): 203-208.
Nuhriawangsa, A. M. P., Z. Bachruddin, Sajidan, and A. Wibowo, 2012.
Aplikasi Fitase dari Bakteri Rekombinan pEAS1-AMP untuk Meningkatkan Kualitas Pakan dan Kinerja Produksi Ayam Broiler yang Ramah Lingkungan. Dalam: Prosiding Seminar Nasional Peternakan Berkelanjutan 4: Inovasi Agribisnis Peternakan untuk Ketahanan Pangan: Komoditas Unggas. Fakultas Peternakan UNPAD, Bandung. Hal: 220-225.
Purwati, C. S., Sajidan, A. Ratriyanto dan A. M. P. Nuhriawangsa. 2016. Seleksi dan optimasi karakter fisik bakteri penghasil fitase yang diisolasi dari sumber air panas di Guci, Tegal. Sains Peternakan. 13(2): 1-6.
Sajidan, R. Wulandari, E. N. Sari, A. Ratriyanto, H. Weldekiros and R. Greiner. 2015. Phytase-producing bacteria from extreme regions in Indonesia. Brazilian Archives of Biology And Technology. 58(5): 711-717.
Wulandari, R., Sajidan dan Suranto. 2011. Analisis Gen 16s rRNA pada Bakteri Penghasil Enzim Fitase. Naskah Publikasi Thesis. Program Studi Magister Biosains, Program Pasca Sarjana, Universitas Sebelas Maret, Surakarta.
Refbacks
- There are currently no refbacks.