Identifikasi Pregnancy-Associated Glycoprotein (PAG) pada Kotiledon Kambing Jawarandu
Abstract
dengan kebuntingan. Molekul PAG disekresikan sebagai salah satu sinyal kebuntingan dari embrio kepada induk sebelum proses implantasi. Penelitian ini bertujuan untuk mengidentifikasi bobot molekul PAG pada kambing Jawarandu. Identifikasi PAG diperoleh dari ekstrak kotiledon yang dikumpukan setelah induk melahirkan. Proses penelitian dilakukan dengan empat tahap, yaitu ekstraksi, purifikasi larutan menggunakan presipitasi asam (H3PO4 1 M, pH 4.7) dan garam ((NH4)2SO4 40% dan 80%), filtrasi larutan menggunakan Sephadex G-75®, dan identifikasi bobot molekul PAG menggunakan SDS-PAGE. Bobot molekul protein yang muncul pada SDS-PAGE pada kolom K-8 menunjukkan adanya dua pita protein dengan bobot molekul 43,61 kDa dan 28,21 kDa. Molekul tersebut diduga dapat digunakan sebagai penanda kebuntingan dini pada kambing Jawarandu.
Kata kunci: PAG, Kotiledon, Kambing Jawarandu, SDS-PAGE
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