Rasio Spermatozoa X:Y dan Kualitas Sperma pada Kambing Kacang dan Peranakan Ettawa

S Bintara

Abstract

The objective of the study was to know the difference of X:Y spermatozoa ratio and sperm quality of Kacang and Ettawa-crossed breed goats. It was conducted at Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science Universitas Gadjah Mada for three months, starting December 2009 to February 2010. Materials of the study were sperm collected from six two-year Ettawa-crossed breed goats weighing 31.5±2.2 kg and from six two-year Kacang goats weighing 18.0±2.1 kg. The sperm was collected using artificial vagina with frequency of twice a week for each goat and it was repeated eight times. The quality and quantity of the sperm was immediately evaluated by its volume, concentration, motility, viability and abnormality. To determine the ratio of X:Y spermatozoa, smear preparation was made and spermatozoa head was then measured using Scion Image software. The results of the measurement were used for identification of X or Y spermatozoa. Spermatozoa having bigger head than the average were identified as X and those having smaller head as Y. The result showed that the ratio of X:Y spermatozoa of Ettawa-crossed breed goats (50.6±1.8:49.4±1.8 %) was not significantly difference with that of Kacang goats
(49.7±1,7:50.3±1.7 %). Sperm volume of Ettawa-crossed breed goats (1.00±0.2 ml) was higher than that of Kacang goats (0.62±0.2 ml). Sperm concentration of Ettawa-crossed breed goats (2,865±431 million/ml) was not significantly difference compared to that of Kacang goats (2,840±383 million/ml). Spermatozoa motility of Ettawa-crossed breed goats (66.7±9.8 %) was higher (P<0.05) than that of Kacang goats (50.0±7.1 %). Spermatozoa viability of Ettawa-crossed breed goats (80.0±7.1 %) was higher (P<0.05) than that of
Kacang goats (67.5±9.4 %). Spermatozoa abnormality of Ettawa-crossed breed goats (8.2±3.3 %) was not significantly difference compared to that of Kacang goats (8.6±2.4 %). The study was concluded that the breeds of Ettawa-crossed breed and Kacang goats have indifferent ratio of X:Y spermatozoa, while the sperm volume, motility and viability of Ettawacrossed breed goats was better than that of Kacang goats.

Key words: XY spermatozoa, quality and quantity, Ettawa-crossed breed, Kacang.

Full Text:

PDF

References

Afiati, F. 2004. Proporsi dan karakteristik spermatozoa X dan Y hasil separasi kolom albumin. Media Peternakan. Fakultas Peternakan IPB, Bogor.

Anonimus. 2007. Scion Image. http://www.scioncorp.com. Accession Date

th Januari 2007.

Astuti, J.M. 1980. Rancangan Percobaan dan Analisa Statistik. Fakultas Peternakan. Universitas Gadjah Mada, Yogyakarta.

Bearden, H.J., J.W. Fuquay and S.T. Willard. 2004. Applied Animal Reproduction. 6nd Edition. Pearson Prentice Hall. Upper Saddle river, New Jersey.

Bintara, S. 2009. Peningkatan Kinerja Reproduksi Induk Kambing Bligon

Melalui Seleksi Pejantan, Identifikasi dan Separasi Spermatozoa, serta Suplementasi Energi-protein. Disertasi. Universitas Gadjah Mada. Yogyakarta.

Chemineau, P., Cagnie Y., Guerin Y., Orgeur P. and Vallet J. C. 1991. Training Manual on Artificial Insemination in Sheep and Goats.

Food Agriculture Organization of The United Nation. Rome, Italy.

Cupps, P.T. 1991. Reproduction in Domestic Animals. Fourth edition. Academic Press Inc. San Diego, New York, Boston, Toronto, London, Sydney, Tokyo.

Delgadillo J. J, Leboeuf B., Chemineau P. 1992. Abolition of seasonal variations in semen quality and maintenance of sperm fertilizing

ability by photoperiodic cycles in goat bucks. Small Ruminant Research 9: 47-59.

Devendra, C. dan M. Burns. 1983. Produksi Kambing di Daerah Tropis. Penerbit ITB dan Penerbit Universitas Udayana.

Ericsson, R.T. 1973. Isolation and storage of progresively motile human sperm. J. Androl., 9: 111-113.

Evans, G and M.W.C. Maxwell. 1987. Salamon’s Artificial Insemination of Sheep and Goat. Butterworths, Sydney.

Gomes, W. R. 1997. Artificial Insemination in : Reproduction in Domestic Animals. H.H. Cole and P.T. Cupps. Academic Press, New York, San Francisco, London.

Hafez, E.S.E. 1993. Reproduction In Farm Animal. Sixth Edition. Lea and Febiger. Philadelphia. Hardjoprandjoto, S. 1995. Ilmu Kemajiran pada

Ternak. Airlangga University Press. Surabaya.

Herdis. 2005. Optimalisasi Inseminasi Buatan Melalui Aplikasi Teknologi Laserpunktur pada Domba Garut (Ovis aries). Disertasi. Institut Pertanian Bogor. Bogor.

Tambing, N.S., Muhamad Gazali dan Bambang Purwantara. 2001. Pemberdayaan teknologi inseminasi buatan pada ternak kambing.

Wartazoa Vol. 11 No. 1 Th. 2001.

Terrill, C. L. 1973. Sheep and Goats in The Artificial Insemination of Farm Animals.Rutgers University Press, New Bruswick, New Jersey.

Toelihere, M.R. 1985. Fisiologi Reproduksi Pada Ternak. Penerbit Angkasa, Bandung.

Toelihere, M.R. 1993. Inseminasi Buatan Pada Ternak. Penerbit Angkasa. Bandung

Werdhani, W. dan B. Lestari. 1996. Peningkatan Produksi Ternak Kambing Lokal Penghasil Daging. Proyek IP2TP. Departemen Pertanian. Yogyakarta.

White, L.M., W.E. Beal, J.H. Bame. R.G. Saacke and C.E. Marshall. 1984. Characteristic of bovine spermatozoa after migration through a bovine serum albumin gradient. J. Anim. Sci., 59: 454-459.

DOI: https://doi.org/10.20961/sainspet.v9i2.4792

Refbacks

  • There are currently no refbacks.