Seleksi dan Optimasi Karakter Fisik Bakteri Penghasil Fitase yang Diisolasi dari Sumber Air Panas di Guci, Tegal

Catur Suci Purwati, Sajidan Sajidan, Adi Ratriyanto, Adi Magna Patriadi Nuhriawangsa

Abstract

The aimed of this study to isolate, select and characterize physically phytase-producing bacteria
from hot springs in the Guci, Tegal. Isolation and enrichment of bacteria using Lurya Betani media
with 2% of phytic acid substrate. Extraction of extracellular phytase thus obtained extract coarse and
optimization by loking at the relative activity at the difference in the resulting product. Phytaseproducing
bacteria can be isolated from the hot springs of the Guci, Tegal with the highest relative
activity in AG2 and AG2-1 colonies. The highest relative activity at a temperature of 55°C, pH 6, the
incubation time of 90 minutes, the substrate concentration of 3% and a metal cofactor Ca2+ at a
concentration of 10-4M. Phytase-producing bacteria can be isolated from a hot spring in Guci, Tegal
and have certain physical characteristics.

Keywords

Bacteria, hot spring, physical characteristics, phytase, relative activity

Full Text:

PDF

References

Berikten, D. and M. Kivanc. 2014.

Optimization of solid-state

fermentation for phytase production

by Thermomyces lanuginosus using

response surface methodology.

Journal Preparative Biochemistry and

Biotechnology. 44(8): 834-848.

Sains Peternakan Vol. 13 (2), 2015

Cowieson, A. J., P. Wilcock and M. R.

Bedford. 2011. Super-dosing effects

of phytase in poultry and other

monogastrics. World's Poultry

Science Journal. 67(2011): 225-235.

Kanpiengjai, A., K. Unban, R.

Prathanaphon and C. Khanongnuch.

Optimal medium and

conditions for phytase production by

thermophilic bacterium,

Anoxybacillus sp. MHW14. Food and

Applied Bioscience Journal. 1(3):

-189.

Kusumadjaja, A. P., T. Budiati, N. N. T.

Puspaningsih dan Sajidan. 2009.

Screening Mikroorganisme

Termofilik Penghasil Enzim Fitase

yang Tumbuh di Kawah Ijen

Banyuwangi. Indonesian Journal of

Chemistry. 9(3):500-504.

Mehta, R., P. Singhal, H. Singh, D. Damle

and A. K. Sharma. 2016. Insight into

thermophiles and their widespectrum

applications.

Biotechnology. 6:81(1-9).

Mittal, A., G. Singh, V. Goyal, A. Yadav, K.

R. Aneja, S. K. Gautam, N. K.

Aggarwal. 2011. Isolation and

biochemical characterization of

acido-thermophilic extracellular

phytase producing bacterial strain for

potential application in poultry feed.

Jundishapur Journal of

Microbiology. 4(4):273-282.

Sajidan. 2002. Molekulare Characterisierung

einer Phytase (Myo-inositol

Hexakifosfate Hydrolase) und von

Fosfatasen aus Bakterieisolaten

Indoneschicher Reisfelder (Klebsiella

pneumoniae). Dissertation. Institut

fuer Biologie. Humboldt Universitat

zu Berlin. Deutschland (Germany).

Sajidan, A. M. P. Nuhriawangsa, A.

Ratriyanto and R. Greiner. 2010.

Isolation and Characterization of

Phytase-Producing Bacteria from

Extreme Regions in Indonesia.

Laporan Hibah Kolaborasi

Internasional. FKIP, UNS, Surakarta.

Spring, P., K. E. Newman, C. Wenk, R.

Messikommer, and M. V. Vranjes.

Effect of pelleting temperature

on the activity of different enzymes.

Poultry Science. 75:357-361

Wulandari, R., Sajidan dan Suranto. 2011.

Analisis Gen 16s rRNA pada Bakteri

Penghasil Enzim Fitase. Naskah

Publikasi Thesis. Program Studi

Magister Biosains, Program Pasca

Sarjana, Universitas Sebelas Maret,

Surakarta.

Zahoor, S., H. M. Javed and M. E. Babar.

Characterization of a novel

hydrolytic enzyme producing

Thermophilic bacterium isolated

from the hot spring of Azad

Kashmir-Pakistan. Brazilian

Archives of Biology and

Technology. 59(2016):1-13.

Zeller, E., M. Schollenberger, M. Witzig, Y.

Shastak, I. Kuhn, L. E. Hoelzle and

M. Rodehutscord. 2015. Interactions

between supplemented mineral

phosphorus and phytase on phytate

hydrolysis and inositol phosphates in

the small intestine of broilers. Poultry

Science. 94:1018-1029.

DOI: https://doi.org/10.20961/sainspet.v13i2.11482

Refbacks

  • There are currently no refbacks.