Evaluasi pakan suplemen terproteksi berdasarkan kecernaan bahan kering, kecernaan bahan organik dan pH secara In Vitro

Ahmad Pramono, Yulia Arnia Sari, Sahrul Romadhon, Muhammad Cahyadi, Dimas Fajar Nugroho

Abstract

Objective: The research was conducted to evaluate protected feed supplement made of soybean groats and worm flour (Lumbricus rubellus) based on in vitro dry matter and organic matter digestibility and pH rumen fluids as a single feed or concentrate.

Methods: The method used in vitro two-stage with observation of stage I (Rumen) and stage II (Total). Completely Randomized Design (CRD) with 6 treatments and 5 replications was used. The treatments were P1 = soybeans groats, P2 = protected soybeans groats, P3 = worm flour, P4 = protected worm flour, P5 = concentrate (50% rice bran + 50% pollard) and P6 = 75% concentrate + 20% protected soybeans groats + 5% protected worm flour. The 37% formaldehyde was used to protect the soybean groats. The addition was determined at 1% from total dry matter. The data obtained were analyzed by Analysis of Variance with Orthogonal Counter Test.

Results: The result obtained for P2 was markedly lower than that of P1 on rumen KcBK and KcBO, while no significant effect was seen concerning the total KcBK and KcBO. Accordingly, similar trend was observed for P4 that was significantly lower in comparison to that of P3. Further, P1 and P2 when compared to P3 and P4 possessed the lowest score among variables. Meanwhile, no effect on variables was seen when P5 compared to P6. Additionally, With regard to P6, the result on KcBK and KcBO were significantly higher compared to remaining treatments.

Conclusions: The results could be concluded that the 37% formaldehyde was used to protect the soybean groats. The addition was determined at 1% from total dry matter can reduce dry matter digestibility and organic matter digestibility of soybean groats and worm flour (Lumbricus rubellus) in the rumen and it can be degraded and digested in the abomasum to the small intestine. Therefore, pH of rumen fluid is optimal, so it does not interfere with the fermentation in the rumen.

Keywords

Nutrient digestibility; Protected feed supplement; pH rumen fluid

References

  1. Widyawati, S. D. 2008. Efek perbedaan sumber protein dan rasio urea-molases dalam pakan suplemen yang ditambahkan dalam ransum terhadap produksi mikrobia rumen secara in vitro. Sains Peternakan. 6(1):34–41. Doi: 10.20961/sains pet.v6i1.4942
  2. Pramono, A., Kustono, D. T. Widayati, P. P. Putro, dan H. Hartadi. 2016. Evaluasi pakan suplemen minyak Ikan Lemuru dan hidrolisat darah terproteksi berdasarkan kecernaan bahan kering dan kecernaan bahan organik di dalam rumen dan pasca rumen. Sains Peternakan. 14(1):36–42. Doi: 10.20961/sainspet.v14i1.8776
  3. Istiqomah, L., A. Sofyan, E. Damayanti, and H. Julendra. 2009. Amino acid profile of earthworm and earthworm meal (Lumbricus rubellus) for animal feedstuff. J. Indones. Trop. Anim. Agric. 34(4):253–257. Doi: 10.14710/jitaa.34.4.253-257
  4. Trisnawati, Y., Suminto, dan A. Sudaryono. 2014. Pengaruh kombinasi pakan buatan dan cacing tanah (Lumbricus rubellus) terhadap efisiensi pemanfaatan pakan, pertumbuhan dan kelulushidupan Lele Dumbo (Clarias gariepinus). Journal of Aquaculture Management and Technology. 3(2):86–93.
  5. Suhartanto, B., R. Utomo, Kustantinah, I. G. S. Budisatria, L. M. Yusiati, dan B. P. Widyobroto. 2014. Pengaruh penambahan formaldehid pada pembuatan undegraded protein aktivitas mikrobia rumen secara in vitro. Buletin Peternakan. 38(3):141–149. Doi: 10.21059/buletinpeternak.v38i3.5249
  6. Wahyono, T., E. Jatmiko, Firsoni, S. N. W. Hardani, dan E. Yunita. 2019. Evaluasi nutrien dan kecernaan in vitro beberapa spesies rumput lapangan tropis di Indonesia. Sains Peternakan. 17(2):17-23. Doi: 10.20961/sainspet.v%vi%i.29776
  7. Utomo, R. 2010. Modifikasi metode penetapan kecernaan in vitro bahan kering atau bahan organik. Bulletin Sintesis. 5:0853–9812.
  8. Mattjik, A. A. dan I. M. Sumertajaya. 2002. Perancangan Percobaan, Jilid I Edisi Kedua. IPB, Bogor.
  9. Aldis, R. E. 2017. Nilai degradasi dan karakteristik fermentasi rumen secara in vitro bahan pakan sumber protein yang diproteksi dengan lama pemanasan berbeda. Universitas Gadjah Mada, Yogyakarta.
  10. Widodo, W. F. dan Sutrisno. 2012. Kecernaan bahan kering, kecernaan bahan organik, produksi VFA dan NH3 pakan komplit dengan level jerami padi berbeda secara in vitro. Animal Agriculture Journal. 1(1):215–230.
  11. Sihombing, G., W. Pratitis, dan G. A. Dewangga. 2010. Pengaruh penggunaan tepung cacing tanah (Lumbricus rubellus) terhadap kecernaan bahan kering dan bahan organik ransum domba lokal jantan. Caraka Tani. 25(1):79–86. Doi: 10.20961/ carakatani.v25i1.15746
  12. Yamashita, S. A., R. D. Rachmat, A. R. Tarmidi, B. Ayuningsih, dan I. Hernaman. 2020. Kecernaan ransum yang mengandung limbah roti pada domba. Jurnal Ilmu dan Teknologi Peternakan Tropis. 7(1):47-51. Doi: 10.33772/jitro.v7i1.9701
  13. Pramono, A., A. Yusuf, S. D. Widyawati, dan H. Hartadi. 2018. Pengaruh suplementasi lemak terproteksi terhadap konsumsi dan kecernaan nutrien sapi perah Friesian Holstein. Sains Peternakan. 16(1):34. Doi: 10.20961/sainspet.v16i1.19700
  14. Pramono, A., R. F. Hadi, J. Sutrisno, and M. Cahyadi. 2019. The effect of protected soybean oil and soybean groats base on in vitro dry matter digestibility, in vitro organic matter digestibility in the rumen and post rumen. IOP Conf. Ser. Earth and Environ. Sci. 347(1). Doi: 10.1088/1755-1315/347/1/012016
  15. Bhorgin, A. J. and K. Uma. 2014. Antimicrobial activity of earthworm powder (Lampito mauritii). Int. J. Curr. Microbiol. App. Sci. 3(1):437-443.
  16. Tulung, Y. L. R., A. F. Pendong, dan B. Tulung. 2020. Evaluasi nilai biologis pakan lengkap berbasis tebon jagung dan rumput campuran terhadap kinerja produksi sapi Peranakan Ongole (PO). Zootec. 40(1):363–379.
  17. Pramono, A., Lutojo, Prayitno, and M Cahyadi. 2019. The effect of protected soybean groats and soybean oil as feed supplement on total gas production. IOP Conf. Ser. Earth Environ. Sci. 250:012027. Doi: 10.1088/1755-1315/250/1/012027
  18. Rustiyana, E., Liman, dan F. Fathul. 2016. Pengaruh substitusi rumput gajah (Pennisetum purpureum) dengan pelepah daun sawit terhadap kecernaan protein kasar dan kecernaan serat kasar pada kambing. Jurnal Ilmiah Peternakan Terpadu. 4(2): 161-165.
  19. Febrina, D. 2012. Kecernaan ransum sapi Peranakan Ongole berbasis limbah perkebunan kelapa sawit yang diamoniasi urea. Jurnal Peternakan. 9(2):68–74.
  20. Suparwi, D. Santoso, dan M. Samsi. 2017. Kecernaan bahan kering dan bahan organik, kadar amonia dan VFA total in vitro suplemen pakan domba. Prosiding Seminar Nasional Pengembangan dan Call of Papers VII. 750–757.
  21. Siti, N.W., N. M. Witariadi, N. K. Mardewi, K. N. N. Candrasih, I. M. Mudita, N. G. K. Roni, I G. L. O. Cakra, dan N. M. S. Sukmawati. 2013. Utilisasi nitrogen dan komposisi tubuh kambing Peranakan Etawah yang diberi pakan hijauan rumput lapangan dengan suplementasi dedak padi. Majalah Ilmiah Peternakan. 16(1).
  22. Wanapat, M., P. Gunun, N. Anantasook, and S. Kang. 2014. Changes of rumen pH, fermentation and microbial population as influenced by different ratios of roughage (rice straw) to concentrate in dairy steers. Journal of Agricultural Science. 152:675–685. Doi:10.1017/S0021859613000658
  23. Nurjana, D. J. 2012. Pengaruh suplementasi asam fulvat terhadap karakteristik fermentasi rumen dan populasi protozoa in vitro. Institut Pertanian Bogor.

DOI: https://doi.org/10.20961/lar.v20i3.64468

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