Morphometric characterization and effect of growth hormone (GH) gene polymorphism on growth traits of Kerinci duck (Anas platyrhynchos)

Salsabila Salsabila, Depison Depison, Silvia Erina

Abstract

Objective: To determine the association of GH gene diversity with growth traits and to obtain the morphometric characteristics in Kerinci ducks.

Methods: The research method is an experiment using 96 blood samples of Kerinci duck blood. The methods included data collection on quantitative characteristics (body weight, body weight gain, body measurements), blood samples from Kerinci ducks, DNA extraction activities, PCR amplification and restriction with AluI enzyme. Data analysis included t-test, T2-Hotelling, principal component analysis, genotype frequency, allele, Hardy-Weinberg balance, heterozygosity, and PIC.

Results: The quantitative characteristics of male Kerinci ducks were significantly different (P<0.05) higher than female Kerinci ducks. Analysis of the Kerinci duck GH|AluI gene was polymorphic. The population of Kerinci ducks was in Hardy-Weinberg equilibrium (P>0.05). Diversity Value of Kerinci Duck Ho<He. Quantitative characteristics of GH gene of Kerinci duck genotype +/+ were significantly different (P<0.05) higher than genotype +/- and -/-.

Conclusions: The quantitative characteristics of male Kerinci ducks were higher than female Kerinci ducks. Characteristics of the body size of Kerinci duck are the length of sternum, length of shank, and circumference of shank, and identifier of body shape is length of wings. The Kerinci duck GH|AluI gene is polymorphic and has associations with quantitative characteristics, with the best genotype being the +/+ genotype.

Keywords

Association; Characterization; Growth Hormone (GH) gene; Kerinci duck

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References

  1. Hartatik, T., D. E. Putra, S. D. Volkandari, T. Kanazawa, and Sumadi. 2018. Genotype analysis of partial growth hormone gene (GH891|MspI) in pesisir cattle and simmental- pesisir crossbred cattle. J. Indonesian Trop. Anim. Agric. 43(l):l-8. Doi: 10.14710/jitaa.43.1.1-8
  2. Brown, T. A. 2016. Gene cloning and DNA analysis: an introduction. hoboken: John Wiley & Sons.
  3. Anjalia, K. M., A. Mandala, B. Gunalanb, L. Rubana, E. Anandajothia, D. Thineshsanthara, T. G. Manojkumara, and S. Kandan. 2019. Identification of six grouper species under the genus Epinephelus (Bloch, 1793) from Indian waters using PCR-RFLP of cytochrome c oxidase I (COI) gene fragment. Food Control. 101(1):39-44. Doi: 10.1016/j.foodcont.2019. 02.024
  4. Moradian C, N. Mohamadi, S. A. R. Sheshdeh, A. Hajihosseinlo, and F. Ashrafi. 2013 Effects of genetic polymorphismat the growth hormone gene on growth traits in Makooei sheep. Eur. J. of Exp. Biol. 3(3):101-105.
  5. Yurnalis, Husmaini, and Sabrina. 2017. Polymorphisms of Growth Hormone Gene Exon 1 and their Associations with Body Weight in Pitalah and Kumbang Janti Ducks. Int. J. Poult. Sci. 16(5):203-208. Doi: 10.392 3/ijps.2017.203.208
  6. Mazurowski, A., A. Frieske., D. Kokoszyñski., S. Mroczkowski., Z. Bernacki, and A. Wilkanowska. 2015. Examination of growth hormone (GH) gene polymorphism and its association with body weight and selected body dimensions in ducks. Folia Biol. 63(1):44-50. Doi: 10.3409/fb63_1.43
  7. Wu, X., Yan, M. J., Lian, S. Y., Liu, X. T. and Li, A. 2014 . GH gene polymorphisms and expression associated with egg laying in muscovy ducks (Cairinamoschata). Hereditas 151:14-19. Doi: 10.1111/j.1601-5223.2013.00016
  8. Wongngam, W., T. Mitani, S. Katayama, S. Nakamura, and J. Yongsawatdigul. 2020. Production and characterization of chicken blood hydrolysate with antihypertensive properties. Poult Sci. 99:5163-5174. Doi: 10.1016/j.psj.2020.07.006
  9. Putri, A. B. S. R. N., D. Gushairiyanto, and D. Depison. 2020. Bobot badan dan karakteristik morfometrik beberapa galur ayam lokal. J. Indonesian Trop. Anim. Agric. 7(3):256-260. Doi: 10.33772/jitro.v7i3.12150
  10. Gaspersz, V. 2006. Teknik analisis dalam pe nelitian percobaan. Penerbit Tarsito. Bandung.
  11. Nei, M. and S. Kumar. 2000. Molecular evolution and phylogenetics. Oxford University Press, New York.
  12. Setiyono, E., and R. P. Bekti. 2019. Karakteristik morfologi dan perkembangan testis itik alabio (anas platyrhynchos borneo) periode grower. Life Sci. 8(2):170-180. Doi: 10.15294/ lifesci.v8i2.37104
  13. Asiamah, C., Y. Xue, L. Lu, K. Zou, Z. Zhao, and Y. Su, 2020. Evaluation of growth performance on family breeding of the Leizhou Black Duck: A preliminary study. Vet. Med. Sci. 6:500-510. Doi: 10.10 02/vms3.263
  14. Khanza, K. N., Gushariyanto, and Depison. 2021. Hubungan antara karakteristik telur dengan bobot telur dan bobot day old duck (dod) dengan bobot badan itik kerinci pada berbagai tingkat umur. Jurnal Ilmu dan Industri Peternakan. 7(2):159-174. Doi: 10.24252/jiip.v7v2.22352
  15. Putra. A., Rukmiasih, and R. Afnan. 2015. Persentase dan kualitas karkas itik cihateup- alabio (ca) pada umur pemotongan yang berbeda. Departemen Ilmu Produksi dan Teknologi Peternakan. 03(1):27-32. Doi: 10.29 244/3.1.27-32
  16. Febrianto, F., Ismoyowati, M. Mufti, Prayitno, and D. Purwantini. 2018. Polymorphism gene GH and morphological characteristic of anas plathyrhyncos and cairina moschata. Anim. Prod. 20(1):17-27. Doi: 10. 20884/1.jap.2018.20.1.665
  17. Prawira, R. Depison, Gushariyanto, and S. Erina. 2021. Hubungan morfologi telur dengan bobot telur dan bobot DOC dengan bobot badan ayam Kampung F1. Jurnal Ilmu Peternakan Terapan. 5(1):19-30. Doi: 10.25047/jipt.v5i1.2728
  18. Rahayu, F. F., Depison, and Gushairiyanto, 2021. Performance of Kampung Super chicken and Bangkok chicken first generation (G1) until the age of 12 weeks. Livest. Anim. Res. 19(3):326-336. Doi: 10.20961/lar. v19i3.52535
  19. Irmaya, D., Depison, and Gushairiyanto, 2021. Quantitative characteristic of Indonesian native chickens at the age of 4 months. Livest. Anim. Res. 19(1):108-119. Doi: 10.20 961/lar.v19i1.43150
  20. Primawati, N., L. O. Nafiu., dan R. Badarudin. 2021. Karakteristik ukuran-ukuran tubuh ayam lokal umur 3-10 minggu pada strain berbeda. Jurnal Ilmiah Peternakan Halu Oleo. 3(1):62-66.
  21. Sitanggang, E. N., Hasnudi, dan Hamdan. 2016. Keragaman sifat kualitatif dan morfometrik antara ayam kampung, ayam bangkok, ayam katai, ayam birma, ayam bagon dan magon di medan. Jurnal Peternakan Integratif. 3(2):167-189. Doi: 10.327 34/jpi.v3i2.2753
  22. Sarbaini, Yurnalis, Hendri, and R. Dahnil, 2018. Analisis keragaman exon-1 gen hormon pertumbuhan pada itik lokal (bayang) sumatera barat menggunakan metoda PCR- RFLP. Jurnal Peternakan Indonesia. 20 (2):124-129. Doi: 10.25077/jpi.20.2.124-129.2018
  23. Nova, T., D. Yurnalis, and A. K. Sari. 2016. Keragaman genetik gen hormon pertumbuhan (gh|mboii) pada itik sikumbang janti menggunakan penciri pcr-rflp. Jurnal Peternakan Indonesia. 18(1):44-52. Doi: 10.25077/jpi.18.1.44-52.2016
  24. Rahmat, M., D. Depison, and E. Wiyanto. 2022. Association of growth hormone gene polymorphism with bodyweight Kampung chicken. Livest. Anim. Res. 20(1):1-10. Doi: 10.20961/lar.v20i1.53736
  25. Suhartati, L., I. Khaerunnisa, A. Gunawan, Rukmiasih, S. Darwati, C. Sumantri, and R. Rizqan. 2020. Short communication: identi fication of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in indonesian kampung and broiler chicken. Biodiversitas J. of Biol. Diver. 21(8):3893-3897. Doi: 10.130 57/biodiv/d210860
  26. Sutopo, S., D. A. Lestari, A. Setiaji, and E. Kurnianto, 2021. Keragaman genotipik entok (Cairina moschata) berdasarkan polimorfisme protein darah. Livest. Anim. Res. 19(3):247-254. Doi: 10.20961/lar.v19i3.54921
  27. Sarbani, A., Jamsari, Arnim, and Yurnalis. 2017. Association of growth hormone gene polymorphism with quantitative characteristic of thin-tailed sheep using pcr-rflp in jambi province. Afr. J. Biotechnol. 16(20):1159-1167. Doi: 10.5897/AJB2016.15783
  28. Akramullah, M., C. Sumantri, N. Ulupi, dan M. A. Pagala. 2020. Identifikasi keragaman gen TGF-Β2 dan asosiasinya dengan sifat pertumbuhan pada ayam tolaki. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan. 8(1):22-29. Doi: 10.29244/jipthp.8.1.22-29
  29. Molee, A., P. Kuadsantia and P. Kaewnakian, 2018. Gene Effects on Body Weight, Carcass Yield, and Meat Quality of Thai Indigenous Chicken. J. Poult. Sci. 55(2):94-102. Doi: 10.2141/jpsa.0160159
  30. Edward, O., D. E. Berryman, E. A. Jensen, P. Kulkarni, S. McKenna, and J. J. Kopchick. 2019. New insights of growth hormone (GH) actions from tissue-specific gh receptor knockouts in mice. Arch Endocrinol Metab. 63(6):557-567. Doi: 10.20945/2359-3997000000185

DOI: https://doi.org/10.20961/lar.v20i3.62609

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