Sensitivity of Pseudomonas sp., from Ettawa Crossbreed Goat (PE) in Special Region of Yogyakarta (DIY) against antibiotic

Widodo Suwito, Widagdo Sri Nugroho

Abstract

Objective: Pseudomonas sp., is bacteria that subclinical mastitis cause in Ettawa crossbreed goat (PE) in special region of Yogyakarta (DIY). Subclinical mastitis in PE goat can be treated with antibiotic during dry period or one week after the milking. The aim of these study was to determined sensitivity Pseudomonas sp., isolate from PE goat subclinical mastitis in DIY against antibiotic that use in the field.

Methods: A total of 23 Pseudomonas sp., isolate on nutrient agar slope were used in this study. All Pseudomonas sp., isolate on nutrient agar slope were cultured in Brain Heart Infusion (BHI) medium and identified based on biochemical reaction. The sensitivity of Pseudomonas sp., isolate against antibiotic tested by diffusion agar with paper discs antibiotic with determine the concentration.

Results: Based on the Minimum Inhibition Concentration (MIC), the Pseudomonas sp., isolate was sensitive tetracycline, oxytetracycline, streptomycin, and resistant sulfamethoxazole.

Conclusions: The PE goat subclinical mastitis disease in DIY which caused by Pseudomonas sp., can be treated with tetracycline, oxytetracycline, and streptomycin.

Keywords

Ettawa crossbreed goat; Subclinical mastitis; Pseudomonas sp; Antibiotic

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References

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