Sensitivity of Pseudomonas sp., from Ettawa Crossbreed Goat (PE) in Special Region of Yogyakarta (DIY) against antibiotic

Widodo Suwito, Widagdo Sri Nugroho

Abstract

Objective: Pseudomonas sp., is bacteria that subclinical mastitis cause in Ettawa crossbreed goat (PE) in special region of Yogyakarta (DIY). Subclinical mastitis in PE goat can be treated with antibiotic during dry period or one week after the milking. The aim of these study was to determined sensitivity Pseudomonas sp., isolate from PE goat subclinical mastitis in DIY against antibiotic that use in the field.

Methods: A total of 23 Pseudomonas sp., isolate on nutrient agar slope were used in this study. All Pseudomonas sp., isolate on nutrient agar slope were cultured in Brain Heart Infusion (BHI) medium and identified based on biochemical reaction. The sensitivity of Pseudomonas sp., isolate against antibiotic tested by diffusion agar with paper discs antibiotic with determine the concentration.

Results: Based on the Minimum Inhibition Concentration (MIC), the Pseudomonas sp., isolate was sensitive tetracycline, oxytetracycline, streptomycin, and resistant sulfamethoxazole.

Conclusions: The PE goat subclinical mastitis disease in DIY which caused by Pseudomonas sp., can be treated with tetracycline, oxytetracycline, and streptomycin.

Keywords

Ettawa crossbreed goat; Subclinical mastitis; Pseudomonas sp; Antibiotic

Full Text:

PDF

References

  1. Marogna, G., C. Pilo, A. Vidili, S. Tola, G. Schianchiand, and S. G. Leori. 2012. Comparison of clinical findings, microbiological results, and farming parameters in goat herds affected by recurrent infectious mastitis. Small Ruminant Res. 102:74-83. Doi:10.1016/j.smallrumres.2011.08.013
  2. Persson, Y. and I. Olofsson. 2011. Direct and indirect measurement of somatic cell count as indicator of intramammary infection in dairy goats. Acta. Vet. Scan. 53:1-5. Doi:10.1186/1751-0147-53-15
  3. Abdalhamed, A. B., G. S. G. Zeedan, and H. A. A. A. Zeina. 2018. Isolation and identification of bacteria causing mastitis in small ruminants and their susceptibility to antibiotics, honey, essential oils, and plant extracts. Veterinary World. Mar; 11:355–362. Doi:10.14202/vetworld.2018.355-362
  4. Suwito, W., Andriani, and W. S. Nugroho. 2019. Isolasi dan identifikasi bakteri dari susu kambing Peranakan Ettawa (PE) terjangkit mastitis subklinis di Kemiri Kebo, Sleman, Yogyakarta. J. Ilmu. Pet. 29:56-64.
  5. Suwito, W., W. S. Nugroho, B. Sumiarto, and A. E. T. H. Wahyuni. 2014. Fakror-faktor risiko mastitis subklinis pada kambing peranakan Ettawah di kabupaten Sleman, Yogyakarta. J. Vet. 15:130-138.
  6. Sela, S., O. Hammer-Muntz, R. Pinto, L. Weisblit, and G. Leitner. 2007. Phenotypic and genotypic characterization of Pseudomonas aeruginosa strains isolated from mastitis outbreaks in dairy herds. J. Dairy Res. 74:425-429. Doi:10.1017/s0022029907002610
  7. Henry, D. and Speert, D. 2011. Pseudomonas. In: Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D, editors. Man. of Clinic. Microbiol. 10th ed. ASM press, Washington, DC. p. 677-691.
  8. CLSI (Clinical and Laboratory Standards Institute). 2012. performance standards for antimicrobial susceptibility testing. Twenty-Second Informational Supplement 32:70-78.
  9. Ikiz, S., B. Basaran, E. B. Bingol, O. Cetin, G. Kasikci, N. Y. Ozgur, M. Ucmak, O. Yilmaz, M. C. Gunduz, and A. Sabuncu. 2013. Presence and antibiotic susceptibility patterns of contagious mastitis agents (Staphylococcus aureus and Streptococcus agalactiae) isolated from milks of dairy cows with subclinical mastitis. Turk. J. Vet. Anim. Sci. 37:569-574. Doi:10.3906/vet-1302-63
  10. Demirci, H., F. Murphy, E. Murphy, T. Steven, Gregory, E. Albert, Dahlberg, and G. Jogl. 2013. A structural basis for streptomycin-induced misreading of the genetic code. Nat. Commun. 4:1355. Doi:10.1038/ncomms2346
  11. Suwito, W., A. E. T. H. Wahyuni, W. S. Nugroho, and B. Sumiarto. 2013. Isolasi dan identifikasi bakteria mastitis klinis pada kambing peranakan Ettawah. Jurnal Sain Veteriner. 31:49-54.
  12. Contreras, A., D. Sierra, A. Sanchez, J. C. Corrales, J. C. Marco, M. J. Paape, and C. Gonzalo. 2007. Mastitis in small ruminants. Small Ruminant Res. 68:145-153. Doi: /10.1016/j.smallrumres.2006.09.011
  13. Sahasranaman, A., and J. L. WoolfordJr. 2013. in Encyclopedia of biological chemistry (2nd ed). Academic Press, Cambridge, Massachusset. p. 116-121. Doi:10.1016/B978-0-12-378630-2-00259-0
  14. Koop, G., C. A. Collar, N. Toft, M. Nielen, T. V. Werven, D. Bacon, and I. A. Gardner. 2013. Risk factors for subclinical intramammary infection in dairy goats in two longitudinal field studies evaluated by bayesian logistic regression. Prev. Vet. Med. 108:304-312. Doi:10.1016/j.prevetmed.2012.11.007
  15. McDougal, S. and F. Anniss. 2005. Efficacy of antibiotic treatment at drying-off in curing existing infections and preventing new infections in dairy goats. In: Hogeveen, H. (Ed.), mastitis in dairy production. Academic Press, Wageningen. p. 523-528.
  16. Petridis, I. G. and G. C. Fthenakis. 2014. Administration of antibiotics to ewes at the beginning of the dry-period. J. Dairy Res. 81:9-15. Doi:10.1017/S0022029913000472
  17. Munita, J. M. and C. A. Arias. 2016. Mechanisms of Antibiotic Resistance. Microbiol. Spectr. 4:0016-2015. Doi:10.1128/microbiolspec.VMBF-0016-2015
  18. Villa, D. F., M. R. Aguilar, and L. Rojo. 2019. Folic Acid Antagonists: Antimicrobial and Immunomodulating Mechanisms and Applications. Int. J. Mol. Sci. 20:2-30. Doi:10.3390/ijms20204996
  19. Indijah, S. W. and P. Fajri. 2016. Farmakologi. Modul bahan cetak ajar farmasi. Kementrian Kesehatan Republik Indonesia, Jakarta.
  20. Tudó, G., E. Rey, S. Borrell, F. Alcaide, G. Codina, and P. Coll. 2010. Characterization of mutations in streptomycin-resistant Mycobacterium tuberculosis clinical isolates in the area of Barcelona. J. Antimicrob. Chemother. 65:2341-2346. Doi:10.1093/jac/dkq322
  21. Ozturk, C. E., A. Sanic, D. Kaya, and I. Ceyhan. 2005. Molecular analysis of isoniazid, rifampin and streptomycin resistance in Mycobacterium tuberculosis isolates from patients with tuberculosis in Duzce, Turkey. Jpn. J. Infect. Dis. 58:309-312. Doi:10.7883/yoken.JJID.2005.309
  22. Scaccabarozzi, L., L. Leoni, A. Ballarini, A. Barberio, C. Locatelli, A. Casula, V. Bronzo, G. Pisoni, O. Jousson, S. Morandi, L. Rapetti, A. García-Fernández, and P. Moroni. 2015. Pseudomonas aeruginosa in dairy goats: genotypic and phenotypic comparison of intramammary and environmental isolates. PloS One. 25:1-23. Doi: 10.1371/journal.pone.0142973
  23. Todar, K. 2008. Bacterial resistance to antibiotics online. [internet]. Bacteriology. [cited 2014 Apr 1]. Available from: http://textbookbacteriology/resistanceantimicrobial.

DOI: https://doi.org/10.20961/lar.v18i2.42928

Refbacks

  • There are currently no refbacks.