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Responses of Dry Matter Digestibility, Organic Matter Digestibility, Gas Production, and Methane Emission of Rice Straw to Ammoniation-Fermentation Methods and Fermentation Duration
Abstract
This study aimed to evaluate the effect of Amofer methods and fermentation duration on the nutritional quality, digestibility, and methane production of rice straw. Two Amofer methods were compared: direct (A1) and indirect (A2), combined with four fermentation periods (0, 7, 14, and 21 days). The results showed that both factors significantly affected dry matter digestibility (DMD), organic matter digestibility (OMD), and gas production (P<0.05). The indirect Amofer method (A2), which involved ammoniation prior to microbial fermentation, produced higher DMD (43.10%) and OMD (44.52%) than the direct method (A1). The best results were obtained from A2B2 (indirect Amofer, 14 days), with the highest DMD (55.96%) and OMD (59.37%), as well as efficient gas production (103.7 mL/g DM) and lower methane emission (1.48 mL CH₄/g DM). Improved digestibility under A2B2 was associated with the breakdown of lignocellulosic bonds during ammoniation and enhanced microbial activity during fermentation. In contrast, extended fermentation (21 days) decreased digestibility and increased methane output. These findings indicate that the indirect Amofer method with 14 days of fermentation optimizes rice straw utilization by enhancing feed digestibility, stimulating rumen microbial efficiency, and reducing methane emissions, contributing to sustainable ruminant nutrition systems.
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Badan Pusat Statistik (BPS). 2024. Produksi Padi (ton). Aceh dalam angka. BPS Provinsi Aceh. [2] Sarnklong, C., Cone, J. W., Pellikaan, W., & Hendriks, W. H. (2010). Utilization of rice straw and different treatments to improve its feed value for ruminants: A review. Asian-Australasian Journal of Animal Sciences, 23(5), 680–692. [3] Wanapat, M., Kang, S., Polyorach, S., & Cherdthong, A. (2013). Role of rumen microbes and fermentation on fibrous feeds with emphasis on rice straw utilization. Tropical Animal Health and Production, 45(7), 1809–1817. [4] Nguyen, T. V., & Ledin, I. (2001). Effects of urea treatment and supplementation with molasses and cassava hay on intake, digestibility and N retention in crossbred growing cattle fed rice straw. Asian-Australasian Journal of Animal Sciences, 14(4), 470–476. [5] Moss, A. R., Jouany, J. P., & Newbold, J. (2000). Methane production by ruminants: Its contribution to global warming. Annales de Zootechnie, 49(3), 231–253. https://hal.science/hal-00889894v1/document. [6] Tilley, J. M. A., & Terry, R. A. (1963). A two-stage technique for the in vitro digestion of forage crops. Journal of the British Grassland Society, 18(2), 104–111. [7] Van Soest, P. J. (2006). Nutritional ecology of the ruminant (2nd ed.). Cornell University Press. [8] McDonald, P., Edwards, R. A., Greenhalgh, J. F. D., Morgan, C. A., Sinclair, L. A., & Wilkinson, R. G. (2010). Animal nutrition (7th ed.). Pearson Education Limited. [9] Wina, E., Susana, I. W. R., & Tangendjaja, B. (2010). Upaya meningkatkan kualitas jerami padi melalui perlakuan kimia dan biologis. Jurnal Wartazoa, 20(2), 55–66. [10] Herath, H. M. I. K., Wickramasinghe, W. M. D. B., & Mapa, R. B. (2004). Use of effective micro-organisms (EM) and urea in accelerating the decomposition of rice straw. Tropical Agricultural Research and Extension, 7, 114–120. https://doi.org/10.4038/tare.v7i0.5418. [11] Muhr, M. J., et al. (2022). Effects of urea-treated rice straw on nutrient digestibility and rumen fermentation in ruminants. Livestock Research for Rural Development, 34(2), Article 34-10. [12] Khan, N. A., Habib, G., & Ullah, G. (2011). Effect of urea-ammoniation on chemical composition and digestibility of wheat straw and maize stover. Pakistan Veterinary Journal, 31(3), 211–214. [13] Wanapat, M., Kang, S., Polyorach, S., & Cherdthong, A. (2013). Role of rumen microbes and fermentation on fibrous feeds with emphasis on rice straw utilization. Tropical Animal Health and Production, 45(7), 1809–1817. [14] Leng, R. A. (2017). Ruminant nutrition and production in the tropics and subtropics. Penambul Books. [15] Torell, R., Bruce, B., & Riggs, W. (2010). Improving the feeding value of wheat straw for beef cattle. University of Nevada Cooperative Extension Fact Sheet, FS-10-23. [16] Rafiee, H., Tahmasbi, A. M., Naserian, A. A., & Valizadeh, R. (2016). Effect of chemical treatment on nutritive value of rice straw. Journal of Animal Science Research, 26(2), 101–109. https://www.tandfonline.com/doi/full/10.1080/15440478.2023.2228486. [17] Muthia, D., Ridla, M., Laconi, E. B., Ridwan, R., Fidriyanto, R., Abdelbagi, M., Harahap, R. P., & Jayanegara, A. (2021). Effects of ensiling, urea treatment and autoclaving on nutritive value and in-vitro rumen fermentation of rice straw. Advances in Animal and Veterinary Sciences, 9(5), 655–661. https://doi.org/10.17582/journal.aavs/2021/9.5.655.661. [18] Getachew, G., Blümmel, M., Makkar, H. P. S., & Becker, K. (2004). In vitro gas measuring techniques for assessment of nutritional quality of feeds: A review. Animal Feed Science and Technology, 72, 261–281. https://www.sciencedirect.com/science/article/abs/pii/S0377840197001892. [19] Ungerfeld, E. M., & Pitta, D. (2024). Review: Biological consequences of the inhibition of rumen methanogenesis. Animal, 26, 101170. https://doi.org/10.1016/j.animal.2024.101170. [20] Akbar, M., Suriyanti, H. S., & Nontji, M. (2022). Pengaruh pemberian dosis Starbio dan lama fermentasi limbah jerami padi terhadap kualitas pakan ternak sapi Bali. Jurnal AgrotekMAS, 3(2), 68–74. https://jurnal.fp.umi.ac.id/index.php/agrotekmas/article/view/248. [21] Xu, Y., Aung, M., Sun, Z., Zhou, Y., Cheng, Y., Hao, L., Padmakumar, V., & Zhu, W. (2022). Bio-Fermentation Improved Rumen Fermentation and Decreased Methane Concentration of Rice Straw by Altering the Particle-Attached Microbial Community. Fermentation, 8(2), 72. https://doi.org/10.3390/fermentation8020072 [22] Blümmel, M., & Ørskov, E. R. (1993). Comparison of in vitro gas production and nylon bag degradability of roughages in predicting feed intake in cattle. Animal Feed Science and Technology, 40(2–3), 109–119. [23] Johnson, K. A., & Johnson, D. E. (1995). Methane emissions from cattle. Journal of Animal Science, 73(8), 2483–2492. https://academic.oup.com/jas/article-abstract/73/8/2483/4632901. [24] Ma, Y., Chen, X., Zahoor Khan, M., Xiao, J., Liu, S., Wang, J., & Cao, Z. (2020). The impact of ammoniation treatment on the chemical composition and in vitro digestibility of rice straw in Chinese Holsteins. Animals, 10(10), 1854. https://doi.org/10.3390/ani10101854. [25] Rosas-Vega, F. E., Pozzan, R., Martínez-Burgos, W. J., Letti, L. A. J., de Mattos, P. B. G., Ramos-Neyra, L. C., … & Soccol, C. R. (2025). Enzymes Produced by the Genus Aspergillus Integrated into the Biofuels Industry Using Sustainable Raw Materials. Fermentation, 11(2), 62. https://doi.org/10.3390/fermentation11020062. [26] Prihartini, I., & Hariska, V. A. (2019). The effect of supplementing lignolitic probiotic in rice straw on in vitro gas production, concentration of ammonia (NH₃), and volatile fatty acid (VFA). International Journal of Engineering & Technology, 8(1.9), 139–143. https://doi.org/10.14419/ijet.v8i1.9.26387. [27] Sasongko, W. T., Larasati, T. R. D., Mulyana, N., & Wahyono, T. (2019) In vitro gas and methane production from fermented rice straw using Trichoderma viride and Phanerochaete chrysosporium inoculant. Dalam IOP Conference Series: Materials Science and Engineering, 546, 022023. https://doi:10.1088/1757-899X/546/2/022023. [28] Jayanegara, A., Krisnawan, N., Widyawati, Y., & Sudarman, A. (2017). Ammoniation of rice straw and supplementation of Paraserianthes falcataria and Sapindus rarak on in vitro rumen fermentation and methane production. Buletin Peternakan, 41(4), 420–430. https://doi.org/10.21059/buletinpeternak.v41i4.25549.
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