Isolasi Fungi Tanah Muara Desa Katialada Gorontalo Lokasi Tiga dan Skrining Aktivitas Antibakteri terhadap Staphylococcus aureus

Nafa Rosyida Zanuba, Muhammad Zainul Arifin, Saeful Akhmad Tauladani, Gani Ahmad Muharram, Asia Asia, Bawon Triatmoko, Lestyo Wulandari, Ari Satia Nugraha

Abstract

Penyalahgunaan antibiotik sebagai agen terapi pada infeksi dapat memberikan ancaman resistensi antibiotik yang mengakibatkan efektifitas pengobatan menjadi kurang optimal. Untuk mengatasi persoalan tersebut, banyak dilakukan penelitian untuk mencari agen antibakteri baru dari bahan alam seperti fungi tanah sebagai pengujian antibakteri. Penelitian ini bertujuan untuk mengetahui potensi antibakteri dari metabolit sekunder fungi tanah terhadap bakteri Staphylococcus aureus yang ditunjukkan melalui nilai persen penghambatan. Penelitian ini diawali dengan mengkultur sampel fungi tanah yang diambil dari desa Katialada, Gorontalo lokasi tiga pada media PDA dan diisolasi pada media PDA baru hingga didapatkan 11 isolat tunggal. Skrining awal aktivitas antibakteri dilakukan dengan metode uji antagonis pada 11 isolat dengan hasil enam isolat diantaraya memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus. Keenam isolat tersebut akan dilakukan fermentasi dan ekstraksi menggunakan etil asetat untuk mendapatkan ekstrak yang akan digunakan pada pengujian antibakteri menggunakan metode mikrodilusi. Hasil pengujian menunjukkan keenam isolat memiliki aktivitas antibakteri terhadap bakteri uji dan direpresentasikan melalui persen penghambatan pada masing-masing isolat sebesar IS2-BTG6-1-1 sebesar 34,6 ± 2,4%; IS3-BTG6-1-3-2 sebesar 42,8 ± 3,1%; IS2-BTG6-3-3 sebesar 41,1 ± 4,4%; IS3-BTG6-1-2-1 sebesar 41,8 ± 2,9%; IS3-BTG6-1-2-2 sebesar 47,8 ± 2,6%; IS2-BTG6-3-3 sebesar 59,2 ± 4,8%.

Keywords

Fungi Tanah Muara; Antibakteri; Staphylococcus aureus

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DOI: https://doi.org/10.20961/jpscr.v10i2.91460

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