PENGARUH KONSENTRASI KATALIS ENZIM GLUKOAMILASE (ASPERGILLUS NIGER) TERHADAP KONSENTRASI PRODUK GLUKOSA PADA KINETIKA REAKSI SIMULTAN SAKARIFIKASI DAN FERMENTASI BIOETANOLDARI SORGUM (SORGHUM BICOLOR L.)

Abstract

Sorghum(Sorghum bicolor L.) is one of raw materials to make bioethanol containing carbohydrate which can be hydrolysis becomeglucose. Making of bioethanol is started by liquification and continued by simultaneous saccharification and fermentation (SSF) by adding glucoamylase (aspergillus niger) enzyme and dry yeast (Saccharomyces cerevisiae). This research aim to know influence of enzyme catalyst concentration inSSF reaction to make bioethanol from sorghum. Reaction time of SSF 6 hours at 30oC by adding glucoamilase enzyme and 8 grams dry yeast with velocity of shaker at 8 scale, after liquification in 2 hours at80oC by adding 2 ml alpha-amilase enzyme. From this research earned relation between concentration of glucoamylase enzyme with concentration of glucose product. Ever greater concentration of glucoamylase enzyme applied, ever greater also concentration of glucose product. From this research, it can be concluded that reaction of SSF with the range of glucoamylase enzyme are 4 ml, 8 ml, 10 ml, and 12 ml for 12% substrate concentration, earned optimum condition is using concentration of glucoamylase enzyme at 8 ml. Michaelis-Menten constant (Km) from this research for 12% substrate concentration and 8 ml glucoamylase enzyme is 11.6875 g/l.hours and maximum velocity of reaction (Vmax) is 12.5 g/l.hours.