PEMBUATAN BIOETANOL DARI ROTI SISA DENGAN PROSES HIDROLISA ENZIMATIS DAN FERMENTASI

Abstract

Bread waste contains 57% carbohydrate, that can be used as the raw material of bioethanol production. This research aims was to study the producing bioethanol process from bread waste, and to determine the effect of catalyst concentration of enzyme glucoamylase (Aspergillus Niger). The bioethanol production with bread waste consisted of three stages: hydrolysis (liquification and sacharification), fermentation and distillation. Saccharification performed for 6 hours at 30oC and pH 4.5 using a variable catalyst enzyme glucoamylase (Aspergillus Niger), with 6, 8, 10, and 12 ml/200 ml of media. The glucose level value in sacharification process is obtained of 12.85 gr/ml, 137.236 gr/ml, 154.82 gr/ml, and 198.0327 gr/ml. The optimum value of glucose level occurs in the glucoamylase (Aspergillus Niger) enzyme addition of 12 ml. In separated hydrolysis and fermentation (SHF) process, there is a separation of glucose formation from starch (hydrolysis) and glucose reduction into bioethanol (fermentation). The enzyme catalyst (Aspergillus Niger) with 6, 8, 10, and 12 ml/200 ml media, it is obtained the bioethanol reduction value respective conditions of 14.1261%, 18.4909%, 24.7592%, and 28.6993%. The optimum condition is obtained in the glucoamylase (Aspergillus Niger) enzyme addition of 12 ml.