KINETIKA PERTUMBUHAN BAKTERI BACILLUS sp. DALAM MEDIUM GLUKOSA SEBAGAI SUMBER KARBON DAN AMONIUM SULFAT SEBAGAI SUMBER NITROGEN

Margono Margono

Abstract

Carbon and nitrogen compounds are macro nutrients for bacterial growth. Nevertheless, its high concentration is potential to inhibit bacterial growth. The objective of this research is to study the effects of gucose and ammonium concentrations to the growth of Bacillus sp. producing polyhydroxybutyrate. The experiments was conducted in shaken flask of 250 mL with working volume of 70 mL. Fermentation was incubated in incubator shaker at environmental temperature, initial pH of 7.0, and shaking speed of 130 rpm. The best growth was happened at glucose concentration of 10 g/L and ammonium concentration of 1.4 g/L. The kinetics constants are μm of 0.148 hour-1, Ks (glucose) of 0.413 g/L, and Ks (ammonium) of 0.038 g/L. At high concentration, glucose and ammonium tended to inhibit growth of the bacteria by different pattern. The inhibition constants of glucose and ammonium are 291.022 g/L and 32.913 g/L respectively.

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References

Ahn, W.S., Park, S.J., and Lee, S.Y., 2000, Production of Poly(3-Hydroxybutyrate) by Fed-Batch Culture of Recombinant Escherichia coli with a Highly Concentrated Whey Solution, Appl. Environ. Microbiol. , 66(8), 3624-3627.

Ahn, W.S., Park, S.J., and Lee, S.Y., 2001, Production of Poly(3-Hydroxybutyrate) from Whey by Cell Recycle Fed-Batch Culture of Recombinant Escherichia coli, Biotechnology Letters, 23, 235-240. Andrews, J.F., 1968, A Mathematical Model for the Continuous Culture of Microorganisms Utilizing Inhibitory Substrates, dalam Bailey, J.E. and Ollis, D.F., 1986, Biochemical Engineering Fundamentals, 2nd ed., p.392, McGraw-Hill Book Company, New York.

Bailey, J.E. and Ollis,D.F., 1986, Biochemical Engineering Fundamentals, 2nd ed., McGraw-Hill Book Co., New York.

Grothe, E. and Chisti, Y., 2000, Poly(β- hydroxybutyric acid) Thermoplastic Production by Alcaligenes latus: Behaviour of Fed-batch Cultures, Bioprocess Engineering, 22, 441-449.

Khanna, S. and Srivastava, A.K., 2005, A Simple Structured Mathematical Model for Biopolymer (PHB) Production, Biotechnol. Prog., 21(3), 830-838.

Nawrath, C., Poirier, Y., and Somerville, C., 1994, Targetting of The Polyhydroxybutyrate Biosynthetic Pathway to The Plastids of Aerobidopsis Thaliana Results in High Levels of Polymer Accumulation, Proc. Natl. Acad. Sci. USA, 91, 12760.

Park, S.J., Park, J.P., and Lee, S.Y., 2002, Production of Poly(3-hydroxybutyrate) from Whey by Fed-batch Culture of Recombinant Escherichia coli in A Pilot- Scale Fermenter, Biotechnology Letters, 24, 185-189.

Reddy, C.S.K., Ghai, R., Rashmi, and Kalia, V.C., 2003, Polyhydroxyalkanoates: an Overview, Bioresource Technology, 87, 137-146.

Shuler, M.L. and Kargi, F., 2002, Bioprocess Engineering Basic Concepts, 2nd ed., Prentice Hall PTR, Upper Saddle River.

Wang, F. and Lee, S.Y., 1997, Poly(3- Hydroxybutyrate) Production with High Productivity and High Polymer Content by a Fed-Batch Culture of Alcaligenes latus under Nitrogen Limitation, Appl. Environ. Microbiol., 63(9) 3703-3706.

DOI: https://doi.org/10.20961/ekuilibrium.v9i2.49539

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