Banana Cultivars Microshoot Induction and Plantlet Formation Using Cytokinin and Auxin

Sugiyono Sugiyono, Prita Sari Dewi, Rendie Prasetyo

Abstract

Banana is a horticultural plant with very high potentials, which contains carbohydrates and vitamins that are useful in fulfilling people's food and nutritional needs. Hence, this study aims to produce superior banana seedlings and develop a protocol for their mass production using a plant in vitro culture technique. This was a two stage-experiment i.e. microshoot production and plantlet formation. The result showed that Gebyar cultivar produced more shoots than the Kepok Kuning cultivar, with an average of 4.25 microshoots explant-1. However, Kepok Kuning produced more leaves than Gebyar, with an average of 4.64 leaves plantlet-1. Banana shoots cultured on the media containing Indole-3-acetic acid (IAA) at a concentration of 2.5 µM produced the highest leaves number. Meanwhile, those cultured on the media containing 1-Naphthalenesacetic acid (NAA) at a concentration of 7.5 µM produced the highest roots number. A Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) up to 30 µM and the one supplemented with 7.5 μM of NAA are suitable for Kepok Kuning and Gebyar cultivars micropropagation with regard to microshoot induction and plantlet formation, respectively.

Keywords

BAP; Gebyar; in vitro culture; Kepok Kuning; NAA; sword sucker

Full Text:

PDF

References

Ahmed, S., Sharma, A., Singh, A. K., Wali, V. K., & Kumari, P. (2014). In vitro multiplication of banana (Musa sp.) cv. Grand Naine. African Journal of Biotechnology, 13(27), 2696–2703. https://doi.org/10.5897/ajb2014.13750

Ali, A., Sajid, A., Naveed, N. H., Majid, A., Saleem, A., Khan, U. A., Jafery, F. I., & Naz, S. (2011). Initiation, proliferation and development of micropropagation system for mass scale production of banana through meristem culture. African Journal of Biotechnology, 10(70), 15731–15738. Retrieved from https://www.ajol.info/index.php/ajb/article/view/97536

Aloni, R. (2010). The induction of vascular tissues by auxin. In: Davies P.J. (eds) Plant Hormones. Dordrecht: Springer. https://doi.org/10.1007/978-1-4020-2686-7_22

Amoo, S. O., & van Staden, J. (2013). Influence of plant growth regulators on shoot proliferation and secondary metabolite production in micropropagated Huernia hystrix. Plant Cell, Tissue and Organ Culture, 112, 249–256. https://doi.org/10.1007/s11240-012-0230-x

Ashraf, M. F., Aziz, M. A., Kemat, N., & Ismail, I. (2014). Effect of cytokinin types, concentrations and their interactions on in vitro shoot regeneration of chlorophytum borivilianum sant. & fernandez. Electronic Journal of Biotechnology, 17(6), 275–279. https://doi.org/10.1016/j.ejbt.2014.08.004

Basri, A. H. (2016). Kajian pemanfaatan kultur jaringan dalam perbanyakan tanaman bebas virus. Agrica Ekstensia, 10(1), 64–73. Retrieved from https://www.polbangtanmedan.ac.id/pdf/Jurnal%202016/Vol%2010%20No%201/08%20Arie.pdf

Bhende, S. S., & Kurien, S. (2015). Sucker production in banana. Journal of Tropical Agriculture, 53(2), 97–106. Retrieved from http://jtropag.kau.in/index.php/ojs2/article/view/339

Bordoloi, N. D. (2016). Factors affecting in vitro shoot-tip culture of banana. Acta Horticulturae, 1113, 151–156. https://doi.org/10.17660/ActaHortic.2016.1113.22

BPS - Statistics Indonesia. (2020). SIMSPH Online, Survey Pertanian Hortikultura yang mencakup data produksi buah-buahan dan sayuran tahunan per kecamatan di seluruh wilayah Indonesia. Badan Pusat Statistik. Retrieved from https://www.bps.go.id/indicator/55/62/1/produksi-tanaman-buah-buahan.html

Buah, J. N., Danso, E., Taah, K. J., Abole, E. A., Bediako, E. A., Asiedu, J., & Baidoo, R. (2010). The effects of different concentrations cytokinins on the in vitro multiplication of plantain (Musa sp.). Biotechnology, 9(3), 343–347. https://doi.org/10.3923/biotech.2010.343.347

Daniells, J. (2007). Global banana disease management - Getting serious with sustainability and food security. Acta Horticulturae, 828, 411–416. https://doi.org/10.17660/ActaHortic.2009.828.43

Feng, J., Shi, Y., Yang, S., & Zuo, J. (2017). Cytokinins. In J. Li, C. Li, S.M. Smith (Ed.), Hormone metabolism and signaling in plants, pp. 77-106. Academic Press. https://doi.org/10.1016/B978-0-12-811562-6.00003-7

Feng, J., Yuan, L., & Bao-zhong, H. (2012). Overview of plant shooting branch. Journal of Northeast Agricultural University (English Edition), 19(2), 74–85. https://doi.org/10.1016/s1006-8104(13)60042-2

Ghag, S. B., Shekhawat, U. K. S., & Ganapathi, T. R. (2015). Fusarium wilt of banana: biology, epidemiology and management. International Journal of Pest Management, 61(3), 250–263. https://doi.org/10.1080/09670874.2015.1043972

Goggin, D. E., Emery, R. J. N., Kurepin, L. V., & Powles, S. B. (2015). A potential role for endogenous microflora in dormancy release, cytokinin metabolism and the response to fluridone in Lolium rigidum seeds. Annals of Botany, 115(2), 293–301. https://doi.org/10.1093/aob/mcu231

Govindaraju, S., Saravanan, J., Jayanthi, B., Nancy, D., & Indra Arulselv, P. (2012). In vitro propagation of Banana (Musa sp.-Rasthali variety) from sword suckers for its commercial production. Research in Plant Biology, 2(5), 1–6. Retrieved from https://updatepublishing.com/journal/index.php/ripb/article/view/2533

Hossain, M. A., Rubel, M. H., Nasiruddin, K. M., & Evamoni, F. Z. (2016). Influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars. Journal of Bioscience and Agriculture Research, 6(2), 553–564. https://doi.org/10.18801/jbar.060216.66

Hu, B., Cao, J., Ge, K., & Li, L. (2016). The site of water stress governs the pattern of ABA synthesis and transport in peanut. Scientific Reports, 6, 1–11. https://doi.org/10.1038/srep32143

Hui, A. V., Bhatt, A., & Keng, C. L. (2012). Micropropagation of Musa acuminata x Musa balbisiana cv. Pisang Awak (ABB genome) and three other cultivars. Pakistan Journal of Botany, 44(2), 777–780. Retrieved from http://www.pakbs.org/pjbot/PDFs/44(2)/46.pdf

Kadhimi, A. A., Alhasnawi, A. N., Mohamad, A., Yusoff, W. M. W., & Zain, C. R. C. M. (2014). Tissue culture and some of the factors affecting them and the micropropagation of strawberry. Life Science Journal, 11(8), 484–493. Retrieved from http://www.lifesciencesite.com/lsj/life1108/063_23503life110814_484_493.pdf

Kebrom, T. H. (2017). A growing stem inhibits bud outgrowth – The overlooked theory of apical dominance. Frontiers in Plant Science, 8, 1–7. https://doi.org/10.3389/fpls.2017.01874

Keller, C. P., Grundstad, M. L., Evanoff, M. A., Keith, J. D., Lentz, D. S., Wagner, S. L., Culler, A. H., & Cohen, J. D. (2011). Auxin-induced leaf blade expansion in Arabidopsis requires both wounding and detachment. Plant Signaling and Behavior, 6(12), 1997–2007. https://doi.org/10.4161/psb.6.12.18026

Kindimba, G., & Msogoya, T. (2014). Effect of benzylaminopurine on in vivo multiplication of French plantain (Musa spp. AAB) cv. ‘Itoke sege.’ Journal of Applied Biosciences, 74(1), 6086–6090. https://doi.org/10.4314/jab.v74i1.1

Lathyfah, U., & Dewi, E. R. S. (2016). Pengaruh variasi konsentrasi Indole Acetid Acid (IAA) terhadap pertumbuhan tunas pisang barangan (Musa acuminata L. triploid AAA.) dalam kultur in vitro. Bioma, 5(1), 32–42. https://doi.org/10.26877/bioma.v5i1.1492

Méndez-Hernández, H. A., Ledezma-Rodríguez, M., Avilez-Montalvo, R. N., Juárez-Gómez, Y. L., Skeete, A., Avilez-Montalvo, J., De-La-Peña, C., & Loyola-Vargas, V. M. (2019). Signaling overview of plant somatic embryogenesis. Frontiers in Plant Science, 10, 1–15. https://doi.org/10.3389/fpls.2019.00077

Müller, D., & Leyser, O. (2011). Auxin, cytokinin and the control of shoot branching. Annals of Botany, 107(7), 1203–1212 https://doi.org/10.1093/aob/mcr069

Mondal, B., Ray, S. K., Misra, D. K., & Khatua, D. C. (2012). Bacterial wilt of banana in West Bengal, India. International Journal of Plant Protection, 5(2), 227–231. Retrieved from http://researchjournal.co.in/online/IJPP/IJPP%205(2)/5_A-227-231.pdf

Müller, D., Waldie, T., Miyawaki, K., To, J. P. C., Melnyk, C. W., Kieber, J. J., Kakimoto, T., & Leyser, O. (2015). Cytokinin is required for escape but not release from auxin mediated apical dominance. Plant Journal, 82(5), 874–886. https://doi.org/10.1111/tpj.12862

Ngomuo, M., Mneney, E., & Ndakidemi, P. (2014). The effect of bud splitting on suppression of apical dominance and inducing multiple buds development in banana shoot tip cultures of cv. ‘Yangambi’ (AAA) in Tanzania. American Journal of Experimental Agriculture, 4(12), 1853–1860. https://doi.org/10.9734/ajea/2014/7290

Novak, S. D., & Whitehouse, G. A. (2013). Auxin regulates first leaf development and promotes the formation of protocorm trichomes and rhizome-like structures in developing seedlings of Spathoglottis plicata (Orchidaceae). AoB PLANTS, 5, 1–12. https://doi.org/10.1093/aobpla/pls053

Nuraini, A., Sumadi, S., & Pratama, R. (2016). Aplikasi sitokinin untuk pematahan dormansi benih kentang G1 (Solanum tuberosum L.). Kultivasi, 15(3), 202–207. https://doi.org/10.24198/kultivasi.v15i3.11765

Pamungkas, S. S. T. (2015). Pengaruh konsentrasi NAA dan BAP terhadap pertumbuhan tunas eksplan tanaman pisang cavendish (Musa paradisiaca L.) melalui kultur in vitro. Gontor AGROTECH Science Journal, 2(1), 31–45. https://doi.org/10.21111/agrotech.v2i1.295

Pillay, M., & Tenkouano, A. (2011). Banana breeding progress and challenges. CRC Press.

Ploetz, R. C. (2015). Management of Fusarium wilt of banana: A review with special reference to tropical race 4. Crop Protection, 73, 7–15. https://doi.org/10.1016/j.cropro.2015.01.007

Prasetyo, R., Sugiyono, & Prayoga, L. (2020). Induksi tunas mikro pisang kultivar ambon nangka (Musa sp.) secara in vitro. Vigor: Jurnal Ilmu Pertanian Dan Subtropika, 5(2), 45–50. Retrieved from https://jurnal.untidar.ac.id/index.php/vigor/article/view/3044

Prayoga, L., & Sugiyono, S. (2010). Uji perbadaan media dan konsentrasi BAP terhadap pertumbuhan tunas pisang raja secara kultur in vitro. Agritech, 12(2), 89–99. Retrieved from http://jurnalnasional.ump.ac.id/index.php/AGRITECH/article/view/990

Qiu, Y., Guan, S. C., Wen, C., Li, P., Gao, Z., & Chen, X. (2019). Auxin and cytokinin coordinate the dormancy and outgrowth of axillary bud in strawberry runner. BMC Plant Biology, 19(1), 1–16. https://doi.org/10.1186/s12870-019-2151-x

Rahman, S., Biswas, N., Hassan, M. M., & Ahmed, M. G., Mamun, A. N. K., Islam, M. R., Moniruzzaman, M., & Haque, M. E. (2013). Micro propagation of banana (Musa sp.) cv. Agnishwar by in vitro shoot tip culture. International Research Journal of Biotechnology, 4(4), 83–88. Retrieved from https://www.interesjournals.org/articles/micro-propagation-of-banana-musa-sp-cv-agnishwar-by-in-vitro-shoot-tip-culture.pdf

Remakanthan, A., Menon, T. G., & Soniya, E. V. (2014). Somatic embryogenesis in banana (Musa acuminata AAA cv. Grand Naine): Effect of explant and culture conditions. In vitro Cellular and Developmental Biology - Plant, 50(1), 127–136. https://doi.org/10.1007/s11627-013-9546-4

Ren, T., Weraduwage, S. M., & Sharkey, T. D. (2019). Prospects for enhancing leaf photosynthetic capacity by manipulating mesophyll cell morphology. Journal of Experimental Botany, 70(4), 1153–1165. https://doi.org/10.1093/jxb/ery448

Satuhu, S., & Supriyadi, A. (2007). Pisang: budi daya, pengolahan, dan prospek pasar. Jakarta: Penebar Swadaya.

Scarpella, E., Barkoulas, M., & Tsiantis, M. (2010). Control of leaf and vein development by auxin. Cold Spring Harbor Perspectives in Biology, 2(1), a001511. https://doi.org/10.1101/cshperspect.a001511

Schaller, G. E., Street, I. H., & Kieber, J. J. (2014). Cytokinin and the cell cycle. Current Opinion in Plant Biology, 21, 7–15. https://doi.org/10.1016/j.pbi.2014.05.015

Singh, W. R., Singh, S. S., & Karuna, S. (2014). Analysis of banana genome groups of wild and cultivated cultivars of Manipur, India using sScore card method. Advances in Applied Science Research, 5(1), 35–38. Retrieved from https://www.imedpub.com/articles/analysis-of-banana-genome-groups-of-wild-and-cultivated-cultivars-ofmanipur-india-using-sscore-card-method.pdf

Sinha, S. K., & Deka, A. C. (2016). Effect of osmotic stress on in vitro propagation of Musa sp. (Malbhog variety). African Journal of Biotechnology, 15(12), 465–471. https://doi.org/10.5897/ajb2015.14446

Skupa, P., Opatrny, Z., & Petrasek, J. (2014). Auxin Biology: Applications and the Mechanisms Behind. In Nick, P., Opatrny Z. (Ed.) Applied Plant Cell Biology: Cellular Tools and Approaches for Plant Biotechnology. Berlin, Heidelberg: Springer. https://doi.org/10.1007/978-3-642-41787-0_3

Sun, J., Wang, M., Lyu, M., Niklas, K. J., Zhong, Q., Li, M., & Cheng, D. (2019). Stem and leaf growth rates define the leaf size vs. number trade-off. AoB PLANTS, 11(6), plz063. https://doi.org/10.1093/aobpla/plz063

Tréhin, C., Planchais, S., Glab, N., Perennes, C., Tregear, J., & Bergounioux, C. (1998). Cell cycle regulation by plant growth regulators: Involvement of auxin and cytokinin in the re-entry of Petunia protoplasts into the cell cycle. Planta, 206(2), 215–224. https://doi.org/10.1007/s004250050393

Viljoen, A., Kunert, K., Kiggundu, A., Escalant, J. V., & Bornman, C.H. (2004). Biotechnology for sustainable banana and plantain production in Africa: The South African contribution. South African Journal of Botany, 70(1), 67–74. https://doi.org/10.1016/S0254-6299(15)30308-2

Vishnevetsky, J., White, T. L., Palmateer, A. J., Flaishman, M., Cohen, Y., Elad, Y., Velcheva, M., Hanania, U., Sahar, N., Dgani, O., & Perl, A. (2011). Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. Grand Nain. Transgenic Research, 20, 61–72. https://doi.org/10.1007/s11248-010-9392-7

Xiong, Y., & Jiao, Y. (2019). The diverse roles of auxin in regulating leaf development. Plants, 8(7), 1–14. https://doi.org/10.3390/plants8070243

Yatim, H. (2016). Multiplication of Raja Bulu banana (Musa paradisiaca L. AAB group) on several Benzyl Amino Purine (BAP) concentration by using in vitro method. Agroekoteknologi, 4(3), 1989–1995. Retrieved from https://www.neliti.com/publications/107456/multiplication-of-raja-bulu-banana-musa-paradisiaca-l-aab-group-on-several-benzy

Zazımalova, E., Murphy, A. S., Yang, H., Hoyerova, K., & Hosek, P. (2010). Auxin transporters-why so many? Cold Spring Harbor Laboratory Press, 2(3), a001552. https://dx.doi.org/10.1101/cshperspect.a001552

Zhao, Y. (2014). Auxin biosynthesis. The Arabidopsis Book, 12(12), e0173. https://doi.org/10.1199/tab.0173

Refbacks

  • There are currently no refbacks.