Modification of Media for Banana In Vitro Propagation with Foliar Fertilizer and Coconut Water in cv. Rajabulu

Siti Mardhikasari, Ahmad Yunus, Samanhudi Samanhudi


The use of foliar fertilizers and coconut water is potential; foliar fertilizer is the alternative medium and coconut water is the plant growth regulator (PGR) in the banana tissue culture. This study aimed to examine the ability of foliar fertilizer to substitute Murashige and Skoog (MS) media and coconut water as the main source of cytokine. There are two factors in this research. The first factor is the media, which consist of three levels, i.e. fully MS, ½ MS + ½ foliar fertilizer and fully foliar fertilizer. The second factor is the coconut water with four levels of concentration i.e. 50, 100, 150 and 200 ml l-1. The results show that fully formulated foliar fertilizer had not been able to substitute or even compete with a fully MS media, but conversely foliar fertilizer was used to substitute ½ MS media. This can be seen from the parameters of shoot length, leaf length, root number and root length with the highest yield that was found in MS media (8.7, 4.66, 3.33 and 3.23 cm). The 50-100 ml l-1 concentrations of coconut water showed the best results by giving a significant effect on the number of roots and root length. The acclimatization of plantlets in this study was nearly 100%, indicated by the formation of a complete organ.


banana; coconut water; foliar fertilizer; in vitro; modification of media

Full Text:



Abdalaziz, Z., Aboul-Nasr, M. H., Dokash, M. H., & Metwaly, A. K. (2016). Using Stimufol® as an Alternative to MS Medium to Vrow Garlic in vitro. International Journal of Agriculture and Economic Development, 4(2), 21–28. Global Strategic Management Inc.

Agampodi, V. A., & Jayawardena, B. (2009). Effect of coconut (Cocos nucifera L.) water extracts on adventitious root development in vegetative propagation of Dracaena purplecompacta L. Acta physiologiae plantarum, 31(2), 279–284. Crossref

Agarwal, M. (2015). Tissue culture of Momordica charantia L.: A review. Journal of Plant Sciences Journal of Plant Sciences. Special Issue: Medicinal Plants, 3(1), 24-32. Retrieved from Link

Akhiriana, E., Samanhudi, & Yunus, A. (2019). Coconut Water and IAA Effect on the In Vitro Growth of Tribulus Terrestris L. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 67(1), 9–18. Crossref

Andini, N. (2016). Penggunaan Air Kelapa dan Ekstrak Buah Pisang Terhadap Multiplikasi Tunas Temulawak (Curcuma xanthoriza Roxb.) Secara In Vitro (Thesis). Surakarta: Program Pascasarjana Universitas Sebelas Maret. Retrieved from Link

Avivi, S., Hardjo Soedarmo, S., & Andi Prasetyo, P. (2013). Multiplikasi Tunas dan Aklimatisasi Tiga Varietas Pisang : Raja Nangka, Kepok, dan Mas, Jurnal Hortikultura Indonesia, 4(2), 83–89. Retrieved from Link

Avivi, S., & Ikrarwati. (2004). Mikropropagasi Pisang Abaca (Musa textillis Nee) Melalui Teknik Kultur Jaringan. Ilmu Pertanian, 10(2). 27-32. Retreived from Link

Bairwa, K. C., Singh, A., Jhajaria, A., Singh, H., Goyam, B. K., Lata, M., & Singh, N. (2015). Conventional sucker with tissue culture banana production in Central India-A case study. African Journal of Agricultural Research, 10(14), 1751–1755. Crossref

Bidhari, L. A., Purwanto, E., & Yunus, A. (2018). Effect of utilization of tomato extract and foliar fertilizer as media on shoots multiplication of banana cv Ambon in vitro. IOP Conference Series: Earth and Environmental Science, 142(1), 1–5. Crossref

Dhanalakshmi, S., & Stephan, R. (2014). Low cost media options for the production of banana (Musa paradisiaca L.) through plant tissue culture. Journal of Academia and Industrial Research (JAIR), 2(9), 509–512. Retrieved from Link

Elisama, M.-L. M., Arturo, V.-V. V., Virginia, C.-A., & Luis, C.-S. (2013). Acclimatization of micropropagated Musa cavendischii cultivar roatan plants submitted to doses of fertigation and auxin. African Journal of Agricultural Research, 8(43), 5335–5340. Retrieved from Link

Eriansyah, M., Susiyanti, S., & Putra, Y. (2018). Pengaruh Pemotongan Eksplan dan Pemberian Beberapa Konsentrasi Air Kelapa terhadap Pertumbuhan dan Perkembangan Eksplan Pisang Ketan (Musa paradisiaca) Secara In Vitro. Agrologia, 3(1), 54–61. Crossref

Gbadamosi, I. T., & Sulaiman, M. O. (2012). The Influence of Growth Hormones and Cocos nucifera Water on the In Vitro Propagation of Irvingia gabonensis (Aubry-Lecomte ex O’Rorke) Baill. Nature and Science Nat Sci, 10(9), 53–58. Retrieved from Link

Ge, L., Peh, C. Y. C., Yong, J. W. H., Tan, S. N., Hua, L., & Ong, E. S. (2007). Analyses of gibberellins by capillary electrophoresis–mass spectrometry combined with solid-phase extraction. Journal of chromatography A, 1159(1–2), 242–249. Crossref

Gebeyehu, A. (2013). Effect of different combinations of 6-benzyl amino purine and naphthalene acetic acid on multiple shoot proliferation of plantain (Musa spp.) cv. Matoke from meristem derived explants. African Journal of Biotechnology, 12(7), 71–80. Retrieved from Link

Gobena, M., Hajare, S. T., & Benti, G. (2018). In vitro Root Development System in Two Popular Cultivars of Banana. International Journal of Current Microbiology and Applied Sciences (IJCMAS), 7(1), 3583–3591. Crossref

Hussein, N. (2012). Effects of nutrient media constituents on growth and development of banana (Musa spp.) shoot tips cultured in vitro. African Journal of Biotechnology, 11(37), 9001–9006. Retrieved from Link

Kahia, J., Ndaruhutse, F., Waweru, B., Bonaventure, N., Mutaganda, A., Sallah, P. Y., Kariuki, N. P., et al. (2015). In vitro propagation of two elite cooking banana cultivars-FHIA 17 and INJAGI. International Journal of Biotechnology and Molecular Biology Research, 6(6), 40–47. Crossref

Kozak, D., & Sałata, A. (2011). Effect of cytokinins on in vitro multiplication of rhubarb (Rheum rhaponticum L.) 'Karpow Lipskiego' shoots and ex vitro acclimatization and growth. Acta Scientiarum Polonorum, Hortorum Cultus, 10(4), 75–87. Retrieved from Link

Kumlay, A. M., & Ercisli, S. (2015). Callus induction, shoot proliferation and root regeneration of potato (Solanum tuberosum L.) stem node and leaf explants under long-day conditions. Biotechnology and Biotechnological Equipment, 29(6), 1075–1084. Crossref

Mahmuddin. (2013). Paradigma Pembangunan Pertanian : Pertanian Berkelanjutan Berbasis Petani Dalam Perspektif Sosiologis. Jurnal Sosiologi Universitas Syiah Kuala, 3(1), 59–76. Retrieved from Link

Manawadu, I., Dahanayake, N., Gamini, S., & Senanayake, N. (2014). Effects of Different Natural Organic Additives on in Vitro Shoot Regeneration of Raphanus sativus L. Var. Beeralu. Journal of Agricultural Science and Technology, 4, 219–223. Retrieved from Link

Michael, P. S. (2011). Effects of coconut water on callus initiation and plant regeneration potentials of sweetpotato. Journal and Proceedings of the Royal Society of New South Wales, 144(3-4), 91–101. Retrieved from Link

Muhammad, K., Gul, Z., Jamal, Z., Ahmed, M., Rehman, A., & Khan, Z. U. (2015). Effect of coconut water from different fruit maturity stages, as natural substitute for synthetic PGR in in vitro potato micropropagation. International Journal of Biosciences (IJB), 6(2), 84–92. Crossref

Mumo, N. N., Rimberia, F. K., Mamati, G. E., & Wanjiku, A. (2013). In vitro regeneration of selected Kenyan papaya (Carica papaya L.) lines through shoot tip culture. African Journal of Biotechnology, 12(49), 6826–6832. Retrieved from Link

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3), 473–497. Crossref

Ngomuo, M., Mneney, E., & Ndakidemi, P. A. (2014). The in vitro propagation techniques for producing banana using shoot tip cultures. American Journal of Plant Sciences, 5(11), 1614–1622. Crossref

Ogero, K. O., Mburugu, G. N., Mwangi, M., Ngugi, M. M., & Ombori, O. (2012). Low Cost Tissue Culture Technology in the Regeneration of Sweet Potato (Ipomoea batatas (L) Lam). Research Journal of Biology, 2(2), 51–58. Retrieved from Link

Oumar, S., Modeste, K. K., Oi, K., Samuel, K., & Edmond, K. K. (2018). Improved in vitro shoot proliferation and rooting of two banana varieties (FHIA-21 and PITA-3). European Journal of Biotechnology and Bioscience, 6(1), 24–29. Retrieved from Link

Prabowo, H., Yuniastuti, E., Samanhudi, & Yunus, A. (2018). Effects of Media Combination With Concentration Of AB-Mix Nutrient On Growth Of Banana Shoots On In Vitro. Bulgarian Journal of Agricultural Science, 24(3), 404–410. Retrieved from Link

Růžička, K., Šimášková, M., Duclercq, J., Petrášek, J., Zažímalová, E., Simon, S., Friml, J., Van Montagu, M. C. E., & Benková, E. (2009). Cytokinin regulates root meristem activity via modulation of the polar auxin transport. Proceedings of the National Academy of Sciences, 106(11), 4284–4289. Crossref

Saad, A. I. M., & Elshahed, A. M. (2012). Plant tissue culture media. Recent advances in plant in vitro culture. Crossref

Samanhudi, Yunus, A., Pujiasmanto, B., & Saras, A. (2018). In Vitro Propagation of Temulawak (Curcuma xanthorrhiza Roxb.). Jurnal Jamu Indonesia, 2(2), 37–42. Crossref

Shekarriz, P., Kafi, M., Deilamy, S. D., & Mirmasoumi, M. (2014). Coconut Water and Peptone Improve Seed Germination and Protocorm Like Body Formation of Hybrid Phalaenopsis. Agriculture Science Developments, 3(10), 317–322. Retrieved from Link

Souza, R. A. V. de, Braga, F. T., Setotaw, T. A., Vieira Neto, J., Azevedo, P. H. de, Azevedo, V. H. de, & Cançado, G. M. de A. (2013). Effect of coconut water on growth of olive embryos cultured in vitro. Ciência Rural, 43(2), 290–296. Crossref

Sridhar, T. M., & Aswath, C. R. (2014). RETRACTED: Review on Medicinal Plants Propagation: A Comprehensive Study on Role of Natural Organic Extracts in Tissue Culture Medium. American Journal of Plant Sciences, 5(20), 3073–3088. Crossref

Sudarshana, M. S., Niranjan, M. H., & Rajashekar, N. (2012). Borassus pulp as substitute for sucrose and vitamins for in vitro cultures of some indian medicinal plants. International Journal of Pharma and Bio Sciences, 3(1), B414–B420. Retrieved from Link

Tafazoli, M., Nasr, S. M. H., Jalilvand, H., & Bayat, D. (2013). Plant regeneration through indirect organogenesis of chestnut (Castanea sativa Mill.). African Journal of Biotechnology, 12(51), 7063–7069. Retrieved from Link

Tian, S., Lu, L., Xie, R., Zhang, M., Jernstedt, J. A., Hou, D., Ramsier, C., et al. (2015). Supplemental macronutrients and microbial fermentation products improve the uptake and transport of foliar applied zinc in sunflower (Helianthus annuus L.) plants. Studies utilizing micro X-ray florescence. Frontiers in Plant Science, 5, 808. Crossref

Tripathi, J. N., Oduor, R. O., & Tripathi, L. (2015). A High-Throughput Regeneration and Transformation Platform for Production of Genetically Modified Banana. Frontiers in Plant Science, 6, 1025. Crossref

Valmayor, R. V., Jamaluddin, S. H., Silayoi, B., Kusumo, S., Danh, L. D., Pascua, O. C., & Espino, R. R. C. (2000). Banana cultivar names and synonyms in Southern Asia. International Network for the Improvement of Banana and Plantain (INIBAP) - Asia and the Pacific Office, Los Banos Laguna, Philippines. Retrieved from Link

Yuniastuti, E., Widodo, C. E., Samanhudi, & Delfianti, M. N. I. (2018). Effect of benzyl amino purine and indole-3-acetic acid on propagation of Sterculia foetida in vitro. IOP Conference Series: Earth and Environmental Science, 142(1), 012011. Crossref

Zinabu, D., Gebre, E., & Daksa, J. (2018). Explants Sterilization Protocol for In-vitro Propagation of Elite Enset (Ensete ventricosum (Welw.) Chessman) Cultivars. Asian Journal of Plant Science and Research, 8(4), 1–7. Retrieved from Link


  • There are currently no refbacks.