Analisis Fenotipik dan Genotipik pada Abnormalitas Klon-klon Kelapa Sawit (Elaeis guineensis Jacq.) Hasil Kultur Jaringan

Endang Yuniastuti


Research to analyze phenotype and genotype of oil palm clone developed from tissue culture which showed normal fruits and abnormal fruits through SDS-PAGE protein technique, and RAPD technique. Result indicated that protein with molecular weight of 100 kDa could differentiate genotype with normal fruit and abnormal fruit from all clones observed. Study on DNA through RAPD technique revealed primers which differentiated genotypes (male flowered genotype, normal fruited genotype, and abnormal fruited genotype) within clone and interclonal, i.e. OPC-07, OPN-16, and SC 10-56. Molecular weight of DNA on zymogram bands pattern were different on each clone.

Genetic similarity among clones and among genotypes were high, more than 85% indicated close genetic distance. Dendogram based on matrix of genetic similarity indicated grouping on each genotype.


phenotype; genotype; tissue culture

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Beer, S.C., Goffreda, J., Phillips, T.D., Murphy, J.P. and Sorrells, M.E., 1993. Assessment of Genetic Variation in Avena sterilis using Morphologicall Traits, Isozymes, and RFLPs. Crop Science, 33(6): 1386-1393.

Direktorat Bina Produksi Perkebunan. 2003. Produksi, Luas Areal, dan Produktivitas Perkebunan di Indonesia 1998-2002 (Online). Available at (verified 20 Agustus 2003).

Durand-Gasselin, T., Duval, Y., Baudouin, L., Maheran, A.B., Konan, E.K. and Noiret, J.M. 1993. Description and degree of the mantled flowering abnormality in oil palm (Elaeis guineensis Jacq.) Clones produced using the ORSTOM-CIRAD procedure. p.33-47. In. V. Rao, I.E. Henson, and Rajanaidu (Ed.). Proc. 1993. ISOPB int. Symp. Recent Dev. In Oil Palm Tissue Culture and Biotechnology. Kuala Lumpur, 24-25 September. 1993.

Ginting, G., R.A. Lubis, dan A.U. Lubis. 1991. Kultur Jaringan Kelapa Sawit (Elaeis guineensis) di Pusat Penelitian Perkebunan Marihat. Seminar Bioteknologi Perkebunan dan Lokakarya Biopolimer PAU-IPB Bogor. p. 115-119.

Grattapaglia, D., Chaparro, J., Wilcox, P., McCord, S., Werner, D., Emmerson, H., McKeand, S., Bridgwater, F., Whetten, R., O’malley, D. and Sederoff, R. 1992. Mapping in woody plants with RAPD markers: applications to breeding in forestry and horticulture. P. 37-40. In: Applications of RAPD Technology to Plant Breeding”. Crop Science Society of America, American Society of Horticultural Science, American Genetic Association.

International Contact Business System (ICBS) Inc. 1997. Studi Tentang Perkebunan dan Pengawasan Minyak Sawit di Indonesia. ICBS. Jakarta.

JICA. 1996. Manual of Protein and DNA Analysis. National Institute of Agrobiological Resources . ministry of Agriculture Forestry and Fisheries. Tsukuba. Japan. 57p.

Jones, L.H. 1991. Endogenous Cytokinins in Oil Palm (Elaeis guineensis L.) Callus, Embryoids and Regerant Plants Measured by Radioimmunoassay. Plant Cell Tissue Organ Cult. 20: 201-210.

Karp, A. 1995. Somaclonal Variation As A Tool for Crop Improvement. Euphytica 85: 295-302.

Kemala, S., dan Wahyudin. 2000. Peluang Investasi Perbenihn Kelapa Sawit. Pusat Penelitian Sosial Ekonomi. Dirjen Kehutanan Dan Perkebunan. Jakarta.

Lubis, A.U. 1992. Kelapa Sawit di Indonesia. Pusat Penelitian Perkebunan Marihat. Bandar Kuala. Sumatra Utara. 435p.

Nei, M. 1987. Estimation of Average Heterozygosity and Genetic Distance from a Small Number of Individuals: Genet. 89: 583-590.

Nienhuis, J., J. Tivang, and P. Skroch. 1994. Analysis of Genetic Relationship Among Genotypes Based on Molecular Marker data. P. 4-8. In: Analysis Molecular Marker Data. Joint Plant Breeding Symposia Series. Corvalis, Or 5-6 August, 1994. CSSA Am Soc. Hort. Sci. And Am Genet. Assoc.

Rohlf, F.J. 1993. NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System Version 1.80. exerter Software. New York.

Tondok, A.R. 1998. Production and Marketing of Indonesia Palm Oil: Past, Present and Future. 1998. Int. Oil palm Conf. Nusa Dua Bali. September 23-25. 1998. Indonesia.

Toruan-Mathius, N, Haris, N., and Ginting, G. 1998. Use of biomolecular techniques in studies of abnormalities in oil palm clones. International Oil Palm Conference. Nusa Dua Bali. 23-25 Sep 1998. Indonesia.

Toruan-Mathius, N, Haris, N., Hutabarat, T., and Ginting, G. 1998. Analisis SDS-PAGE, IEF Protein dan RAPD pada Daun dan Buah Tanaman Kelapa Sawit Abnormal. Laporan Intern Unit Penelitian Bioteknologi Perkebunan. 10p.


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