Sri Widadi, Linayanti Darsana, Sumijati Sumijati


Black rot disease which is caused by Xanthomonas campestris pv campetris is animportant disease on cabbage and so far could not be controlled satisfactory yet.Recently, people getting conscious the negative effect of applicating synthetic pesticidesand the importance of using biocontrol agents fo controlling pests because they arerelatively save and environmentally friendly. Bacteriophage is viruse that infectsbacteria. The use of phages for disease control is a fast expanding area of plantprotection with great potential to replace the chemical control and now prevalent.Phages can be used effectively as part of integrated disease management strategies.The relative ease of preparing phage treatments and low cost of production of theseagents make them good candidates for widespread use in developing countries as well.So far, in Indonesia bacteriophage exploration for development as biocontrol agents hasnot been conducted yet. On the other hand, Indonesia is one of view countries having alot of biodiversity resourches in the world. So it can be predicted that it is a lot ofbacteriophages strains naturally, which could be developed for biocontrol agents ofblack rot disease in cabbage. This research was aimed to isolate some bacteriophagevirulent to Xanthomonas campestris pv campetris from field. X. campestris pv campetris has been isolated from diseased cabbage in Blumbang, Tawangmangu.Biological characterization assay of 10 diseased cabbage leaf samples showed that allsample were indicated innfected by X. campestris pv campetris. Whereas plaque assayof 10 samples taken from fields showed indication of plague formation. This researchstill be continued to explore more samples form Tawangmangu, and also from othercabbage field in Central Java.


Bacteriophage; exploration; biocontrol; Xanthomonas; cabbage

Full Text:



Balogh B, Jones JB, Momol MT, OlsonSM, Obradovic A. 2003. Improvedefficacy of newly formulatedbacteriophages for management ofbacterial spot on tomato. Plant Dis.87:949 54

Balogh, B. 2002. Strategies for improvingthe efficacy of bacteriophages forcontrolling bacterial spot of tomato.Mster Thesis. Graduate School of theUniversity of Florida.

Civerolo, E.L and Keil, H.L. 1969.Inhibition of bacterial spot of peachfoliage by Xanthomonas prunibacteriophage. Phytopathology59:1966 67

Flaherty JE, Jones JB, Harbaugh BK,Somodi GC, Jackson LE. 2000.Control of bacterial spot on tomato in

the greenhouse and .eld with Hmutant bacteriophages. HortScience35:882 84

Jones, J.B., L.E. Jackson. B. Balogh, A.Obradovic, F.B. Iriarte, and M.T.Momol. 2007. Bacteriophages forPlant Disease Control.. Annu. Rev.Phytopathol..45:245-262.

Momol MT, Jones JB, Olson SM,Obradovic A, Balogh B, King P.2002. Integrated management ofbacterial spot on tomato in Florida. Rep. PP110, EDIS, Inst. Food Agric.Sci., Univ. FL. Online.

Obradovica A., Jones, JB, Momol MT,Olson SM, and Jackson LE. 2005.Integration of biological controlagents and systemic acquiredresistance inducers against bacterialspot on tomato. Plant Dis. 89:712 16

Obradovic A, Jones JB, Momol MT,Balogh B, and Olson SM. 2004.Management of tomato bacterial spotin the .eld by foliar applications ofbacteriophages and SAR inducers.

Plant Dis. 88:736 40

Sambrook, J. and D.W. Russel. 2001.Molecular Cloning. A. LaboratoryManual. Third Edition. Cold SpringHarbor Laboratory Press. Cold SpringHarbor, New York. p2.25-3.49.

Semangun, H. 1996. Pengantar IlmuPenyakit Tumbuhan. Gadjah MadaUniversity Press. Yogyakayta.

Streets, R.B. 1972. Diagnosis of PlantDiseases. The University of ArizonaPress, USA.


  • There are currently no refbacks.