Response of Barley (Hordeum vulgare L.) Growth, Yield, and Crop Components to Irrigation Frequency and Salicylic Acid Concentrations

Raheem Bohan Al-Nasr-Allah, Muaid Shaker Ali, Dunya Sabeeh Mohammed

Abstract

The irrigation regime is one of the primary factors limiting barley productivity. This study aimed to investigate the effect of salicylic acid on barley performance under varying irrigation conditions. A field experiment was conducted during the winter season of 2024 to 2025 using a split-plot design arranged in a randomized complete block design (RCBD) with 3 replicates. Irrigation frequency (3, 4, and 5 times during the growing season) was assigned to the main plots, while salicylic acid concentrations (0, 100, 200, and 300 mg l-1) were distributed to the subplots. The studied traits included the number of tillers per plant, plant height, flag leaf area, relative water content of leaf, absolute growth rate, biomass duration, 100-grain weight, number of grains per spike, and grain yield. The results showed that increasing irrigation frequency positively enhanced all studied traits except relative water content, with the 5-irrigation treatment resulting in the highest grain yield (1.383 kg m-2). Spraying salicylic acid at a concentration of 300 mg l-1 considerably improved most growth and yield traits, with the highest grain yield (1.370 kg m-2), compared with the control treatment, which recorded the lowest grain yield (0.959 kg m-2). Strong positive correlations were observed among biomass accumulation, yield components, and grain yield. The combined application of 5 irrigations and 300 mg l-1 of salicylic acid resulted in the highest grain yield (1.668 kg m-2). These findings suggest that appropriate irrigation management combined with salicylic acid during barley development can be an effective strategy to enhance barley productivity.

Keywords

drought stress; foliar application; grain yield; physiological traits; water deficit

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References

Abdelaal, K. A., Attia, K. A., Alamery, S. F., El-Afry, M. M., Ghazy, A. I., Tantawy, D. S., ... & Hafez, Y. M. (2020). Exogenous application of proline and salicylic acid can mitigate the injurious impacts of drought stress on barley plants associated with physiological and histological characters. Sustainability, 12(5), 1736. https://doi.org/10.3390/su12051736

Alsahli, A., Mohamed, A-K., Alaraidh, I., Al-Ghamdi, A., Al-Watban, A., El-Zaidy, M., & Alzahrani, S. M. (2019). Salicylic acid alleviates salinity stress through the modulation of biochemical attributes and some key antioxidants in wheat seedlings. Pakistan Journal of Botany, 51(5), 1551–1559. http://dx.doi.org/10.30848/PJB2019-5(12)

Alsamadany, H., Abdulbaki, A. S., & Alzahrani, Y. (2024). Unravelling drought and salinity stress responses in barley genotypes: Physiological, biochemical, and molecular insights. Frontiers in Plant Science, 15, 1417021. https://doi.org/10.3389/fpls.2024.1417021

Ahmad, H., Khan, I., Liaqat, W., Jan, M. F., & Ahmadzai, M. D. (2018). Effect of salicylic acid on yield and yield component of maize under reduced irrigation. International Journal of Environmental Sciences & Natural Resource, 9(2), 76–80. Retrieved from https://juniperpublishers.com/ijesnr/pdf/IJESNR.MS.ID.555763.pdf

Ahmad, A., Aslam, Z., Naz, M., Hussain, S., Javed, T., Aslam, S., ... & Jamal, M. A. (2021). Exogenous salicylic acid-induced drought stress tolerance in wheat (Triticum aestivum L.) grown under hydroponic culture. PLoS ONE, 16(12), e0260556. https://doi.org/10.1371/journal.pone.0260556

Akbari, M., Sabouri, H., Sajadi, S. J., Yarahmadi, S., Ahangar, L., Abedi, A., & Katouzi, M. (2022). Mega meta-QTLs: A strategy for the production of golden barley (Hordeum vulgare L.) tolerant to abiotic stresses. Genes, 13(11), 2087. https://doi/10.3390/genes13112087

Aktas, N., Farouk, S., Al-Ghamdi, A. A. M., Alenazi, A. S., AlMalki, M. A. L., & Dinler, B. S. (2025). Pipecolic acid, a drought stress modulator, boosts chlorophyll assimilation, photosynthetic performance, redox homeostasis, and osmotic adjustment of drought-affected Hordeum vulgare L. seedlings. Plants, 14(13), 1949. https://doi.org/10.3390/plants14131949

ASGIS. (2025). Wheat and barley production report. Authority of Statistics and Geographic Information system of Iraq. Retrieved from https://cosit.gov.iq/ar/

Asma, Hussain, I., Ashraf, M. Y., Saleem, M. H., Ashraf, M. A., Ali, B., … & Yasin, G. (2023). Alleviating effects of salicylic acid spray on stage-based growth and antioxidative defense system in two drought-stressed rice (Oryza sativa L.) cultivars. Turkish Journal of Agriculture and Forestry, 47(1), 79–99. https://doi.org/10.55730/1300-011X.3066

Barr, H. D., & Weatherly, P. E. (1962). A re-examination of the relative turgidity techniques for estimating water deficits in leaves. Australian Journal of Biological Sciences, 15(3), 431–428. https://doi.org/10.1071/BI9620413

Black, C. A. (1965). Methods of soil analysis. Part I, American Society of Agronomy, p. 1572. Madison, Wisconsin, USA. Retrieved from https://archive.org/details/methodsofsoilana0002blac/page/n7/mode/2up

Chen, S., Zhao, C. B., Ren, R. M., & Jiang, J. H. (2023). Salicylic acid had the potential to enhance tolerance in horticultural crops against abiotic stress. Frontiers in Plant Science, 14, 1141918. https://doi.org/10.3389/fpls.2023.1141918

dos Santos, T. B., Ribas, A. F., de Souza, S. G. H., Budzinski, I. G. F., & Domingues, D. S. (2022). Physiological responses to drought, salinity, and heat stress in plants: A review. Stresses, 2(1), 113–135. https://doi.org/10.3390/stresses2010009

FAO. (2023). FAOSTAT database. Food and Agriculture Organization of the United Nations. Retrieved from https://www.fao.org/faostat/

Farouk, S., Arafa, S. A., & Nassar, R. M. A. (2018). Improving drought tolerance in corn (Zea mays L.) by foliar application with salicylic acid. International Journal of Environment, 7(3), 104–123. Retrieved from https://www.curresweb.com/ije/ije/2018/104-123.pdf

Ganj-Abadi, F., Rad, A. H. S., Sani, B., & Mozafari, H. (2021). Grain yield and qualitative of rapeseed genotypes change in response to exogenous application of salicylic acid and planting density. Gesunde Pflanzen, 73(3), 335–344. https://doi.org/10.1007/s10343-021-00558-2

Geng, L., Li, M., Zhang, G., & Ye, L. (2022). Barley: A potential cereal for producing healthy and functional foods. Food Quality and Safety, 6, fyac012. https://doi.org/10.1093/fqsafe/fyac012

Hanif, S., Mahmood, A., Javed, T., Bibi, S., Zia, M. A., Asghar, S., ... & Ali, B. (2024). Exogenous application of salicylic acid ameliorates salinity stress in barley (Hordeum vulgare L.). BMC Plant Biology, 24(1), 270. https://doi.org/10.1186/s12870-024-04968-y

Ignatenko, A. A., Batova, Y. V., Kholoptseva, E. S., & Kaznina, N. M. (2023). Influence of presowing treatment of seeds with salicylic acid on growth and photosynthetic apparatus of barley with different zinc contents in substrate. Russian Journal of Plant Physiology, 70(3), 35. https://doi.org/10.31857/S001533032370001X

Kalra, G. S., & Dhiman, S. D. (1977). Determination of leaf area of wheat plants by rapid method. Journal of the India Botanical Society, 58, 261–264. Retrieved from https://scholar.google.com/scholar?cites=14259368820988124268&as_sdt=2005&sciodt=0,5&hl=en

Kathwal, R., Thakral, S. K., Kumar, P., Sharma, K. D., Sharma, K. Sh., Jindal, Y., & Kumar, A. (2022). Effect of plant regulators on growth, yield attributes, yield and economics in wheat under restricted irrigation. Annals of Agri-Bio Research, 27(1), 17–22. Retrieved from https://agribiop.com/wp-content/uploads/2019/11/AOABR-27-1-17-22.pdf

Keshavarz, Y., Alizadeh, O., Sharfzade, S., Zare, M., & Bazrafshan, F. (2019). Experimental analysis for affecting the exogenous salicylic acid on drought tolerance in maize in Iran. Revista Turismo Estudos e Práticas-RTEP/UERN, 1, 1–8. Retrieved from http://geplat.com/rtep/index.php/tourism/article/view/234

Keshavarz, Y., Alizadeh, O., Sharfzade, Sh., Zare, M., & Bazrafshan, F. (2020). The effect of biological fertilizer and salicylic acid on the physiological and biological characteristics of corn under drought stress. EurAsian Journal of Biosciences, 14(1), p1603. Retrieved from https://openurl.ebsco.com/EPDB%3Agcd%3A16%3A32733696/detailv2?sid=ebsco%3Aplink%3Ascholar&id=ebsco%3Agcd%3A146289660&crl=c&link_origin=scholar.google.com

Khalvandi, M., Siosemardeh, A., Roohi, E., & Keramati, S. (2021). Salicylic acid alleviated the effect of drought stress on photosynthetic characteristics and leaf protein pattern in winter wheat. Heliyon, 7(1), e05908. https://doi.org/10.1016/j.heliyon.2021.e05908

Khan, M. I. R., Poor, P., & Janda, T. (2022). Salicylic acid: A versatile signaling molecule in plants. Journal of Plant Growth Regulation, 41(5), 1887–1890. https://doi.org/10.1007/s00344-022-10692-4

Kvent, J., Svobal, J., & Fiala, K. (1969). Canopy development in stands of Typha latifolia L. and Phragmites communis Trin. in South Moravia. Hydrobiologia, 10, 63–75. Retrieved from https://scholar.google.com/scholar?cites=16748681115891474727&as_sdt=2005&sciodt=0,5&hl=en

Kopecká, R., Kameniarová, M., Černý, M., Brzobohatý, B., & Novák, J. (2023). Abiotic stress in crop production. International Journal of Molecular Sciences, 24(7), 6603. https://doi.org/10.3390/ijms24076603

Langridge, P. (2018). Economic and academic importance of barley. The barley genome (pp. 1–10). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-319-92528-8_1

Liu, J., Qiu, G., Liu, C., Li, H., Chen, X., Fu, Q., … & Guo, B. (2022). Salicylic acid, a multifaceted hormone, combats abiotic stresses in plants. Life, 12(6), 886. https://doi.org/10.3390/life12060886

Maqsood, M. F., Shahbaz, M., Zulfiqar, U., Saman, R. U., Rehman, A., Naz, N., ... & Haider, F. U. (2023). Enhancing wheat growth and yield through salicylic acid-mediated regulation of gas exchange, antioxidant defense, and osmoprotection under salt stress. Stresses, 3(1), 372–386. https://doi.org/10.3390/stresses3010027

Moustakas, M., Sperdouli, I., Adamakis, I-DS., Moustaka, J., İşgören, S., & Şaş, B. (2022). Harnessing the role of foliar applied salicylic acid in decreasing chlorophyll content to reassess photosystem II photoprotection in crop plants. International Journal of Molecular Sciences, 23(13), 7038. https://doi.org/10.3390/ijms23137038

Oguz, M. C., Aycan, M., Oguz, E., Poyraz, I., & Yildiz, M. (2022). Drought stress tolerance in plants: Interplay of molecular, biochemical and physiological responses in important development stages. Physiologia, 2(4), 180–197. https://doi.org/10.3390/physiologia2040015

Page, A. L., Miller, R. H., & Keeney, D. R. (1982). Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties. American Society of Agronomy. In Soil Science Society of America, Vol. 1159. Retrieved from https://scholar.google.com/scholar?cites=6608438278023448955&as_sdt=2005&sciodt=0,5&hl=en

Statista. (2024). World barley production from 2008/2009 to 2024/2025. Retrieved from http://www.statista.com/statistics/271973/world-barley-production

Sharma, A., Kohli, S. K., Khanna, K., Ramakrishnan, M., Kumar, V., Bhardwaj, R., ... & Zheng, B. (2023). Salicylic acid: A phenolic molecule with multiple roles in salt-stressed plants. Journal of Plant Growth Regulation, 42(8), 4581–4605. https://doi.org/10.1007/s00344-022-10902-z

Shemi, R., Wang, R., Gheith, E. S. M., Hussain, H. A., Cholidah, L., Zhang, K., ... & Wang, L. (2021). Role of exogenous-applied salicylic acid, zinc and glycine betaine to improve drought-tolerance in wheat during reproductive growth stages. BMC Plant Biology, 21(1), 574. https://doi.org/10.1186/s12870-021-03367-x

Tesfaye, E. L., & Bayih, T. (2024). Four Ethiopian barley (H. vulgare) varieties with a range of tolerance to salinity and water stress. Rhizosphere, 29, 100841. https://doi.org/10.1016/j.rhisph.2023.100841

USDA. 2025. Iraq Production, Supply and Distribution (PSD) Online Database. United States Department of Agriculture. Retrieved from https://apps.fas.usda.gov/psdonline/

Wassie, M., Zhang, W., Zhang, Q., Ji, K., Cao, L., & Chen, L. (2020). Exogenous salicylic acid ameliorates heat stress-induced damages and improves growth and photosynthetic efficiency in alfalfa (Medicago sativa L.). Ecotoxicology and Environmental Safety, 191, 110206. https://doi.org/10.1016/j.ecoenv.2020.110206

Yakubu, Y., Aliyu, Z. Q., Usman, A., & Evans, O. (2020). Split-plot central composite experimental design method for optimization of cake height to achieve desired texture. Nigerian Journal of Basic and Applied Sciences, 28(1), 30–39. http://dx.doi.org/10.4314/njbas.v28i1.5

Zinati, Z., Sazegari, S., Tahmasebi, A., & Delavari, A. (2021). A comprehensive meta-analysis to identify transcriptional signatures of abiotic stress responses in barley (Hordeum vulgare). Cereal Research Communications, 49(3), 385–391. https://doi.org/10.1007/s42976-020-00107-z

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