Response of Growth and Yield of Mint (Mentha spicata) Cuttings to Auxin and Composition of Planting Media

Eddy Triharyanto, Bambang Pujiasmanto, Pardono Pardono, Aisyah Taqiyyah Fa’izah

Abstract

Consumption of herbal medicines by Indonesian people is increasing because of the price and ease of obtaining them. One of the main ingredients of herbal medicine is mint leaves because they contain tannin and flavonoid secondary metabolites. However, the generative mint plant is very slow and needs vegetative cuttings propagation. This study aims to examine the effect of auxin-type growth regulators and the composition of the growing media on the growth and yield of mint plant cuttings. This study used a complete randomized block design with two factors. The first factor was the auxin growth regulator with four levels: without growth regulator, IBA 500 ppm, IAA 500 ppm, NAA 500 ppm. The second factor was the composition of the planting medium with three levels, namely soil, husk charcoal, and goat manure 3:3:1; 3:2:2, and 3:1:3. Three repetitions were carried out. Auxin affects the number and area of leaves. Applying 500 ppm NAA encouraged the highest leaf growth, with the number of leaves at 163.38. Growth regulator NAA 500 ppm increased the number of leaves and leaf area of mint plants, i.e., by 0.01% and 1.16% compared to the control. The growing media composition affected the crown fresh weight, root biomass, and crown root ratio. The media composition of 3:3:1 produced a crown fresh weight of 41.07 g, and the media composition 3:3:3 produced the highest biomass of 5.97 g. The media composition 3:1:3 showed the highest root crown ratio, 2.69%. Auxin and the composition of the growing media synergistically promote the growth and yield of mint cuttings.

Keywords

6-Benzyl Amino Purine; Crown root ratio; Indole Acetic Acid; Naphthalene Acetic Acid; Root biomass

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References

Adewoyin JA, Arimoro FO. 2023. Animal manure as a biostimulant in bioremediation of oil-contaminated soil: the role of earthworms. Environ Monit Assess. 195(2). https://doi.org/10.1007/s10661-022-10884-1.

Ahmad I, Song X, Hussein Ibrahim ME, Jamal Y, Younas MU, Zhu G, Zhou G, Adam Ali AY. 2023. The role of melatonin in plant growth and metabolism, and its interplay with nitric oxide and auxin in plants under different types of abiotic stress. Front Plant Sci. 14(February):1–12. https://doi.org/10.3389/fpls.2023.1108507.

Anyanwu OA, Naumova EN, Chomitz VR, Zhang FF, Chui K, Kartasurya MI, Folta SC. 2022. The Effects of the COVID-19 Pandemic on Nutrition, Health and Environment in Indonesia: A Qualitative Investigation of Perspectives from Multi-Disciplinary Experts. Int J Environ Res Public Health. 19(18). https://doi.org/10.3390/ijerph191811575.

Boeckx P, Bauters M, Dewettinck K. 2020. Poverty and climate change challenges for sustainable intensification of cocoa systems. Curr Opin Environ Sustain. 47:106–111. https://doi.org/10.1016/j.cosust.2020.10.012.

Brahmi F, Lounis N, Mebarakou S, Guendouze N, Yalaoui-Guellal D, Madani K, Boulekbache-Makhlouf L, Duez P. 2022. Impact of Growth Sites on the Phenolic Contents and Antioxidant Activities of Three Algerian Mentha Species (M. pulegium L., M. rotundifolia (L.) Huds., and M. spicata L.). Front Pharmacol. 13(June):1–13. https://doi.org/doi:10.3389/fphar.2022.886337

Budiastuti MTS, Supriyono, Manurung IR, Setyaningrum D, Nurmalasari AI, Arista NID. 2021. The role of organic fertilizer from natural dye waste and mycorrhizal inoculation on the growth of Indigofera tinctoria L. IOP Conf Ser Earth Environ Sci. 905(1):012011. https://doi.org/doi:10.1088/1755-1315/905/1/012011.

Caren GJ, Iskandar D, Pitaloka DAE, Abdulah R, Suwantika AA. 2022. COVID-19 Pandemic Disruption on the Management of Tuberculosis Treatment in Indonesia. J Multidiscip Healthc. 15:175–183. https://doi.org/doi:10.2147/JMDH.S341130.

Chun Wang, Mei Qi, Jiameng Guo, Chengxu Zhou, Xiaojun Yan , Roger Ruan PC. 2022. Efficient Detection , and Their Adversity Resistance Applications. Molecules.:1–15.

Damodaran S, Strader LC. 2019. Indole 3-butyric acid metabolism and transport in Arabidopsis thaliana. Front Plant Sci. 10(July):1–9. https://doi.org/doi:10.3389/fpls.2019.00851

Deswiniyanti NW, Lestari NKD, Astarini IA, Hardini Y. 2020. In Vitro Propagation of Lily (Lilium longiflorum Thunb.) with Growth Regulators BAP and NAA. J Hortik Indones. 11(2):131–139. https://doi.org/doi:10.29244/jhi.11.2.131-139

Fauk NK, Seran AL, Raymond C, Merry MS, Tahir R, Asa GA, Ward PR. 2022. Why Do We Not Follow Lifesaving Rules? Factors Affecting Nonadherence to COVID-19 Prevention Guidelines in Indonesia: Healthcare Professionals’ Perspectives. Int J Environ Res Public Health. 19(14). https://doi.org/doi:10.3390/ijerph19148502

Gomes GLB, Scortecci KC. 2021. Auxin and its role in plant development: structure, signalling, regulation and response mechanisms. Plant Biol. 23(6):894–904. https://doi.org/doi:10.1111/plb.13303

Gorjian H, Mihankhah P, Khaligh NG. 2022. Influence of tween nature and type on physicochemical properties and stability of spearmint essential oil (Mentha spicata L.) stabilized with basil seed mucilage nanoemulsion. J Mol Liq. 359:119379. https://doi.org/10.1016/j.molliq.2022.119379.

Graceson A, Hare M, Monaghan J, Hall N. 2013. The water retention capabilities of growing media for green roofs. Ecol Eng. 61(PA):328–334. http://dx.doi.org/10.1016/j.ecoleng.2013.09.030.

Harahap FS, Walida H, Rahmaniah R, Rauf A, Hasibuan R, Nasution AP. 2020. Pengaruh Aplikasi Tandan Kosong Kelapa Sawit dan Arang Sekam Padi terhadap beberapa Sifat Kimia Tanah pada Tomat.

Hussain MJ, Abbas Y, Nazli N, Fatima S, Drouet S, Hano C, Abbasi BH. 2022. Root Cultures, a Boon for the Production of Valuable Compounds: A Comparative Review. Plants. 11(3). https://doi.org/doi:10.3390/plants11030439.

Iftikhar J, Lyu M, Liu Z, Mehmood N, Munir N, Ahmed MAA, Batool W, Aslam MM, Yuan Y, Wu B. 2020. Sugar and hormone dynamics and the expression profiles of sut/suc and sweet sweet sugar transporters during flower development in petunia axillaris. Plants. 9(12):1–19. https://doi.org/doi:10.3390/plants9121770

de Jesús Romo-Paz F, Folgado R, Delgado-Aceves L, Zamora-Natera JF, Portillo L. 2021. Tissue culture of Physalis angulata L. (Solanaceae): techniques for micropropagation and germplasm long-term preservation. Plant Cell Tissue Organ Cult. 144(1):73–78. doi:10.1007/s11240-020-01970-8. https://doi.org/10.1007/s11240-020-01970-8

Jha RK, Thapa R, Shrestha AK. 2022. Effect of GA3 and NAA on tomato production under protected cultivation in Kaski, Nepal. J Agric Food Res. :100450. https://doi.org/10.1016/j.jafr.2022.100450

Kapczyńska A, Kowalska I, Prokopiuk B, Pawłowska B. 2020. Rooting media and biostimulator goteo treatment effect the adventitious root formation of pennisetum ‘vertigo’ cuttings and the quality of the final product. Agric. 10(11):1–12. https://doi.org/doi:10.3390/agriculture10110570

Li Y, Huang J, Yu C, Mo R, Zhu Z, Dong Z, Hu X, Zhuang C, Deng W. 2023. Physiological and Transcriptome Analyses of Photosynthesis in Three Mulberry Cultivars within Two Propagation Methods (Cutting and Grafting) under Waterlogging Stress. Plants. 12(11). https://doi.org/doi:10.3390/plants12112066

Liu H, Bai Y, Huang C, Wang Y, Ji Y, Du Y, Xu L, Yu DG, Bligh SWA. 2023. Recent Progress of Electrospun Herbal Medicine Nanofibers. Biomolecules. 13(1). https://doi.org/doi:10.3390/biom13010184

Luo C, Wang Y, Zhang X, Zhang W, Liu H. 2022. Spatial prediction of soil organic matter content using multiyear synthetic images and partitioning algorithms. Catena. 211(January):106023.. https://doi.org/10.1016/j.catena.2022.106023

Ma Q, Wang M, Zheng G, Yao Y, Tao R, Zhu M, Ding J, Li C, Guo W, Zhu X. 2021. Twice-split application of controlled-release nitrogen fertilizer met the nitrogen demand of winter wheat. F Crop Res. 267:108163. https://doi.org/10.1016/j.fcr.2021.108163.

Mokhtarikhah G, Ebadi MT, Ayyari M. 2022. Agro-morphological and phytochemical studies of spearmint landraces (Mentha spicata L.) in Iran. Ind Crops Prod. 176:114367. https://doi.org/10.1016/j.indcrop.2021.114367

Nardi S, Schiavon M, Francioso O. 2021. Chemical structure and biological activity of humic substances define their role as plant growth promoters. Molecules. 26(8). https://doi.org/doi:10.3390/molecules26082256.

Nezhad M, Alibeigi B, Estaji A, Gruda N, Aliniaeifard S. 2022. Growth, Biomass Partitioning, and Photosynthetic Performance of Chrysanthemum Cuttings in Response to Different Light Spectra. Plants. 3337(11).

Pasternak T, Palme K, Pérez-Pérez JM. 2023. Role of reactive oxygen species in the modulation of auxin flux and root development in Arabidopsis thaliana. Plant J. 114(1):83–95. doi:10.1111/tpj.16118.

Perico C, Tan S, Langdale JA. 2022. Developmental regulation of leaf venation patterns: monocot versus eudicots and the role of auxin. New Phytol. 234(3):783–803. https://doi.org/doi:10.1111/nph.17955

Rehman A, Qamar R, Safdar ME, Rehman AU, Ahmad S, Nadeem MA, Hassan MU. 2022. Role of Plant Growth Promoters on Sugarcane Production Propagated Through Budchips in Semiarid Region of Pakistan. J Plant Environ. 3(2):137–146. https://doi.org/doi:10.33687/jpe.003.02.3768

Ren X, Wang Z, Zhao M, Xie J, Zhang Z, Yang F, Wang Q, Ding Y. 2022. Role of selenite on the nitrogen conservation and greenhouse gases mitigation during the goat manure composting process. Sci Total Environ. 838(May):155799. https://doi.org/10.1016/j.scitotenv.2022.155799.

Sardar H, Waqas M, Naz S, Ejaz S, Ali S, Ahmad R. 2022. Evaluation of different growing media based on agro-industrial waste materials for the morphological, biochemical and physiological characteristics of stevia. Clean Waste Syst:100038. https://doi.org/10.1016/j.clwas.2022.100038.

Shekhawat JK, Rai MK, Shekhawat NS, Kataria V. 2021. Synergism of m-topolin with auxin and cytokinin enhanced micropropagation of Maytenus emarginata. Vitr Cell Dev Biol - Plant. 57(3):418–426. https://doi.org/doi:10.1007/s11627-020-10132-6.

Silva BN, Cadavez V, Caleja C, Pereira E, Calhelha RC, Añibarro-Ortega M, Finimundy T, Kostić M, Soković M, Teixeira JA, et al. 2023. Phytochemical Composition and Bioactive Potential of Melissa officinalis L., Salvia officinalis L. and Mentha spicata L. Extracts. Foods. 12(5):1–15. https://doi.org/doi:10.3390/foods12050947

Singh AK, Singh R, Kumar R, Gupta AK, Kumar H, Rai A, Kanawjia A, Tomar KS, Pandey G, Singh B, et al. 2023. Evaluating Sustainable and Environment Friendly Growing Media Composition for Pot Mum (Chrysanthemum morifolium Ramat.). Sustain. 15(1). https://doi.org/doi:10.3390/su15010536.

Small CC, Degenhardt D. 2018. Plant growth regulators for enhancing revegetation success in reclamation: A review. Ecol Eng. 118(April):43–51. https://doi.org/10.1016/j.ecoleng.2018.04.010.

Sodiq AH, Setiawati MR, Santosa DA, Widayat D. 2019. The potency of bio-organic fertilizer containing local microorganism of Cibodas village, Lembang-West Java. In: IOP Conference Series: Earth and Environmental Science. Vol. 383. Institute of Physics Publishing.

Sosnowski J, Malinowska E, Jankowski K, Król J, Redzik P. 2019. An estimation of the effects of synthetic auxin and cytokinin and the time of their application on some morphological and physiological characteristics of Medicago x varia T. Martyn. Saudi J Biol Sci. 26(1):66–73. https://doi.org/doi:10.1016/j.sjbs.2016.12.023.

Suharjo, Nadja RA. 2021. Correlation between carbohydrate content, protein, and fat with compatibility of durian seed grafting (durio zibethinus. Murr). IOP Conf Ser Earth Environ Sci. 681(1). https://doi.org/doi:10.1088/1755-1315/681/1/012013.

Tan CW, Zhang PP, Zhou XX, Wang ZX, Xu ZQ, Mao W, Li WX, Huo ZY, Guo WS, Yun F. 2020. Quantitative monitoring of leaf area index in wheat of different plant types by integrating NDVI and Beer-Lambert law. Sci Rep. 10(1):1–10. https://doi.org/doi:10.1038/s41598-020-57750-z.

Uchida Y, Moriizumi M, Shimotsuma M. 2019. Effects of rice husk biochar and soil moisture on the accumulation of organic and inorganic nitrogen and nitrous oxide emissions during the decomposition of hairy vetch (Vicia villosa) mulch. Soil Sci Plant Nutr. 65(4):409–418. https://doi.org/10.1080/00380768.2019.1624139

Vives-Peris V, de Ollas C, Gómez-Cadenas A, Pérez-Clemente RM. 2020. Root exudates: from plant to rhizosphere and beyond. Plant Cell Rep. 39(1):3–17. https://doi.org/10.1007/s00299-019-02447-5

Wang H, Ouyang Q, Yang C, Zhang Z, Hou D, Liu H, Xu H. 2022. Mutation of OsPIN1b by CRISPR/Cas9 Reveals a Role for Auxin Transport in Modulating Rice Architecture and Root Gravitropism. Int J Mol Sci. 23(16). https://doi.org/doi:10.3390/ijms23168965.

Xing N, Huang W, Xie Q, Shi Y, Ye H, Dong Y, Wu M, Sun G, Jiao Q. 2020. A transformed triangular vegetation index for estimating winter wheat leaf area index. Remote Sens. 12(1):1–19. https://doi.org/doi:10.3390/RS12010016.

Yu J, Zhang Y, Ning S, Ye Q, Tan H, Chen R, Bu Q, Zhang R, Gong P, Ma X, et al. 2019. Molecular cloning and metabolomic characterization of the 5-enolpyruvylshikimate-3-phosphate synthase gene from Baphicacanthus cusia. BMC Plant Biol. 19(1):1–11. https://doi.org/doi:10.1186/s12870-019-2035-0

Zhang Q, Gong M, Xu X, Li H, Deng W. 2022. Roles of Auxin in the Growth, Development, and Stress Tolerance of Horticultural Plants. Cells. 11(17). https://doi.org/doi:10.3390/cells11172761

DOI: https://doi.org/10.20961/agrotechresj.v7i1.76700

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