Pengaruh Pemberian Berbagai Konsentrasi Indole-3-Butyric Acid (IBA) dan Lama Perendaman terhadap Pertumbuhan Stek Batang Timi (Thymus vulgaris L.)
Abstract
The method of thyme stem cutting needs to be developed to produce better roots to support growth. The study aims to examine the response of thyme stem cuttings to various concentration of IBA and time soaking. The research was carried out on January – March 2020 in screen house PT. INDMIRA, Sleman, Yogyakarta and the Physiology and Plant Breeding Laboratory of the Faculty of Animal and Agricultural Science, Diponegoro University. The method used was a completely randomized design with a factorial 4×3 with 5 replications. The first factor was a various concentration of IBA: A0 (0 ppm), A1 (100 ppm), A2 (250 ppm), and A3 (500 ppm). The second factor was the time soaking: B1 (5 minutes), B2 (10 minutes), and B3 (15 minutes.) The results showed that IBA concentration has no significant effect for root dry weight, while the time soaking has significant effect for all parameters. Soaking thyme stem cuttings at an IBA 250 ppm for 10 minutes gave the best results on plant length, the number of leaves, root length, and root dry weight, but soaking in water (without IBA) for 5 minutes was able to give the best number of percentage of life cuttings (85%) so that giving IBA had no needed.
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Aderounmu, A. F. (2019). Effects of stock age, hormone types and concentrations on rooting and early growth of Vitellaria paradoxa C. F. Gaertn. Stem Cuttings. Journal of Advances in Biology & Biotechnology, 22(2), 1–9. https://doi.org/10.9734/jabb/2019/v22i230109
Alpriyan, D., & Karyawati, A. S. (2018). Pengaruh konsentrasi dan lama perendaman hormon auksin pada bibit tebu (Saccharum officinarum L.) teknik bud chip. Jurnal Produksi Tanaman, 6(7), 1354–1362.
Amri, E., Lyaruu, H. V. M., Nyomora, A. S., & Kanyeka, Z. L. (2010). Vegetative propagation of african blackwood (Dalbergia melanoxylon Guill. & Perr.): effects of age of donor plant, IBA treatment and cutting position on rooting ability of stem cuttings. New Forests, 39(2), 183–194. https://doi.org/10.1007/s11056-009-9163-6
Amri, Ezekiel. (2011). The effect of auxins (IBA , NAA) on vegetative propagation of medicinal plant. Tanzania Journal of Natural and Applied Sciences (TaJONAS), 2(2), 359–366.
Apriliani, A., Noli, Z. aneloi, & Suwiren. (2015). Pemberian beberapa jenis dan konsentrasi auksin untuk menginduksi perakaran pada stek pucuk bayur (Pterospermum javanicum Jungh.) dalam upaya perbanyakan tanaman revegetasi. Jurnal Biologi Universitas Andalas (J. Bio. UA.), 4(3), 178–187.
Arimarsetiowati, R., & Ardiyani, F. (2012). Pengaruh penambahan auxin terhadap pertunasan dan perakaran kopi arabika perbanyakan somatic embryogenesis. Pelita Perkebunan (a Coffee and Cocoa Research Journal), 28(2), 82–90. https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v28i2.201
Arlianti, T., Syahid, S. F., Kristina, N. N., & Rostiana, O. (2014). Pengaruh auksin IAA, IBA, dan NAA terhadap induksi perakaran tanaman stevia (Stevia rebaudiana) secara in vitro. Buletin Penelitian Tanaman Rempah Dan Obat, 24(2), 57–62. https://doi.org/10.21082/bullittro.v24n2.2013.
Arumugam, G., Sinniah, U. R., Swamy, M. K., & Lynch, P. T. (2020). Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) sprengel, an aromatic medicinal plant. In Vitro Cellular and Developmental Biology - Plant, 56(4), 491–503. https://doi.org/10.1007/s11627-020-10056-1
Brondani, G. E., Baccarin, F. J. B., de Wit Ondas, H. W., Stape, J. L., Gonçalves, A. N., & de Almeida, M. (2012). Low temperature, IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings. Journal of Forestry Research, 23(4), 583–592. https://doi.org/10.1007/s11676-012-0298-5
Dauqan, E. M. A., & Abdullah, A. (2017). Medicinal and functional values of thyme (Thymus vulgaris L.) Herb. Journal of Applied Biology & Biotechnology, 5(02), 17–22. https://doi.org/10.7324/jabb.2017.50203
De Almeida, M. R., Aumond, M., Da Costa, C. T., Schwambach, J., Ruedell, C. M., Correa, L. R., & Fett-Neto, A. G. (2017). Environmental control of adventitious rooting in Eucalyptus and Populus cuttings. Trees - Structure and Function, 31(5), 1377–1390. https://doi.org/10.1007/s00468-017-1550-6
Delcheh, K. S., Kashefi, B., & Mohammadhassan, R. (2014). A review optimization of tissue culture medium medicinal plant : Thyme. International Journal of Farming and Allied Sciences, 3(9), 1015–1019.
Denaxa, N. K., Vemmos, S. N., & Roussos, P. A. (2021). Shoot girdling improves rooting performance of kalamata olive cuttings by upregulating carbohydrates, polyamines and phenolic compounds. Agriculture (Switzerland), 11(1), 1–16. https://doi.org/10.3390/agriculture11010071
Djamhari, S. (2012). Memecah Dormansi Rimpang Temulawak (Curcuma xanthorrhiza Roxb) menggunakan larutan atonik dan stimulasi perakaran dengan aplikasi auksin. Jurnal Sains dan Teknologi Indonesia, 12(1), 66–70. https://doi.org/10.29122/jsti.v12i1.852
Elhaak, M., & Gad, D. (2015). Propagation principles in using Indole-3-Butyric Acid for rooting rosemary stem cuttings. Journal of Horticulture, 02(01). https://doi.org/10.4172/2376-0354.1000121
Fahly, M., Barus, A., & Haryati. (2017). Pengaruh beberapa komposisi media tanam dan konsentrasi IBA (Indole Butiric Acid) terhadap pertumbuhan setek basal daun mahkota tanaman nenas (Ananas comosus L. Merr.). Jurnal Agroteknologi FP USU, 5(4), 854–859.
Hafizah, N. (2014). Pertumbuhan stek mawar (Rosa damascena Mill.) pada waktu perendaman dalam larutan urine sapi. Ziraa’ah, 39(3), 129–135.
Hossain, M. A., Islam, M. A., & Hossain, M. M. (2004). Rooting ability of cuttings of Swietenia macrophylla King and Dillenia indica L. as influenced by exogenous hormone. International Journal of Agriculture and Biology, 6(April 2015), 560–564.
Hussain, K., Qadri, R., Akram, M. T., Nisar, N., Iqbal, A., Yang, Y., Khan, M. M., Imran-Ul-Haq, Khan, R. I., & Iqbal, M. A. (2020). Clonal propagation of olive (Olea europeae) through semi- hardwood cuttings using IBA under shaded polyethylene tunnels (SPTS). Fresenius Environmental Bulletin, 29(9 A), 8131–8137.
Jan, I., Iqbal, A., Bhutta, M., & Ahmad, N. (2015). Effect of various concentra tions of indole butyric acid (IBA) on olive cuttings. mitt Eilungen Klosterneuburg, 65: 49–55.
Javed, H., Erum, S., Tabassum, S., & Ameen, F. (2013). An overview on medicinal importance of Thymus vulgaris. Journal of Asian Scientific Research, 3(10), 974–982.
Juárez-Rosete, C., Aguilar-Castillo, J., & Rodriguez-Mendoza, M. (2014). Fertilizer source in biomass production and quality of essential oils of thyme (Thymus vulgaris L.). European Journal of Medicinal Plants, 4(7), 865–871. https://doi.org/10.9734/ejmp/2014/9169
Kamila, P. K., Das, P. K., Mohapatra, P. K., & Panda, P. C. (2020). Effect of auxins on rooting of stem cuttings in Hypericum gaitii. Journal of Herbs, Spices and Medicinal Plants, 26(4), 423–434. https://doi.org/10.1080/10496475.2020.1749207
Karimi, M., Berrichi, A., & Boukroute, A. (2014). Study of vegetative propagation by cuttings of Thymus satureioides. Journal of Materials and Environmental Science, 5(4), 1320–1325.
Kaushik, S., & Shukla, N. (2020). Effect of IBA and NAA and their combination on the shooting of stem cuttings of african marigold (Tagetes erecta L.) cv. pusa narangi gainda. International Journal of Chemical Studies, 8(3), 1800–1802. https://doi.org/10.22271/chemi.2020.v8.i3y.9462
Kim, S. H., Kim, J. H., Oh, H. J., Kim, S. Y., & Suh, G. U. (2021). Vegetative propagation of Veronica dahurica and Veronica pusanensis by stem cuttings with auxins. Rhizosphere, 17, 100315. https://doi.org/10.1016/j.rhisph.2021.100315
Kosakowska, O., Węglarz, Z., & Bączek, K. (2021). The Effect of open field and foil tunnel on yield and quality of the common thyme (Thymus vulgaris L.), in organic farming. Agronomy, 11(2), 197. https://doi.org/10.3390/agronomy11020197
Kumar, S., Malik, A., Yadav, R., & Yadav, G. (2019). Role of different rooting media and auxins for rooting in floricultural crops: A review. International Journal of Chemical Studies, 7(2), 1778–1783.
Lakehal, A., Chaabouni, S., Cavel, E., Le Hir, R., Ranjan, A., Raneshan, Z., Novák, O., Păcurar, D. I., Perrone, I., Jobert, F., Gutierrez, L., Bakò, L., & Bellini, C. (2019). A molecular framework for the control of adventitious rooting by TIR1/AFB2-Aux/IAA-Dependent auxin signaling in Arabidopsis. Molecular Plant, 12(11), 1499–1514. https://doi.org/10.1016/j.molp.2019.09.001
Martínez, M. T., Vieitez, F. J., Solla, A., Tapias, R., Ramírez-Martín, N., & Corredoira, E. (2020). Vegetative propagation of Phytophthora cinnamomi-tolerant holm oak genotypes by axillary budding and somatic embryogenesis. Forests, 11(8). https://doi.org/10.3390/F11080841
Mehta, S., Singh, K., Singh Harsana, A., & Mehta, C. S. (2018). Effect of IBA concentration and time of planting on rooting in pomegranate (Punica granatum) cuttings. Journal of Medicinal Plants Studies JMPS, 25053(61), 250–253. http://www.plantsjournal.com/archives/2018/vol6issue1/PartD/6-1-30-120.pdf
Mishra, P., Roggen, A., Ljung, K., & Albani, M. C. (2020). Natural variation in adventitious rooting in the alpine perennial arabis alpina. Plants, 9(2), 1–9. https://doi.org/10.3390/plants9020184
Mulyani, C., & Ismail, J. (2015). Pengaruh konsentrasi dan lama perendaman rootone f terhadap pertumbuhan stek pucuk jambu air (Syzygium semaragense) Pada Media Oasis. Agrosamudra, 2(2), 1–9.
Mulyono, D. (2012). Pengaruh zat pengatur tumbuh auksin: indole butiric acid (IBA) dan sitokinin: benzil amino purine (BAP) dan kinetin dalam elongasi pertunasan gaharu (Aquilaria beccariana). Jurnal Sains dan Teknologi Indonesia, 12(1), 1–7. https://doi.org/10.29122/jsti.v12i1.842
Muthulakshmi, V., Vijayam, C. V., Bachpai, V. K. W., Sivakumar, V., Muthulakshmi, E., Shanmugavel, S., Muneera Parveen, A. B., Yasodha, R., Rajasugunasekar, D., Nagarajan, B., Mayavel, A., & Ghosh Dasgupta, M. (2020). Genetic control of adventitious rooting traits in bi-parental pedigree of Eucalyptus tereticornis × E. camaldulensis. New Forests, 0123456789. https://doi.org/10.1007/s11056-020-09810-5
Netopilova, M., Houdkova, M., Urbanova, K., Rondevaldova, J., & Kokoska, L. (2021). Validation of qualitative broth volatilization checkerboard method for testing of essential oils: Dual‐column gc–fid/ms analysis and in vitro combinatory antimicrobial effect of origanum vulgare and thymus vulgaris against staphylococcus aureus in liquid and vapor phases. Plants, 10(2), 1–23. https://doi.org/10.3390/plants10020393
Nguyen, T. Q., Nguyen, B. H., Thi, D. H. T., Thi, O. D., Nguyen, Q. N., & Vo, C. C. (2020). Rooted cuttings by IBA (indole butyric acid) and NAA (Naphthalene acetic acid) in black pepper (piper nigrum) - A case study in Vietnam. International Journal of Chemical Studies, 8(3), 1880–1885. https://doi.org/10.22271/chemi.2020.v8.i3z.9480
Okafor, C. U., Njoku, E. U., Ike, F. C., & Onyekwuluje, C. C. (2020). Indole-3-Butyric acid induces plant regeneration from stem cuttings of three medicinal plants. Nigerian Journal of Biotechnology, 37(1), 109–121. https://doi.org/10.4314/njb.v37i1.12
Pacholczak, A., & Nowakowska, K. (2020). The effect of biostimulators and indole-3-butyric acid on rooting of stem cuttings of two ground cover roses. Acta Agrobotanica, 73(1), 1–9. https://doi.org/10.5586/aa.7314
Paradiković, N., Zeljković, S., Tkalec, M., Vinković, T., Dervić, I., & Marić, M. (2013). Influence of rooting powder on propagation of sage (Salvia officinalis L.) and rosemary (Rosmarinus officinalis L.) with green cuttings. Poljoprivreda, 19(2), 10–15.
Rahardjo, R., Darwati, I., & Nurhayati, H. (2020). Pengaruh pemupukan terhadap pertumbuhan, produksi, dan mutu tanaman timi (Thymus vulgaris L.). Jurnal Penelitian Tanaman Industri, 20(4), 195. https://doi.org/10.21082/jlittri.v20n4.2014.195-202
Rao, G. S. K., Bisati, I. A., Sharma, A., Kosser, S., Bhat, S. A., & Bisati, A. (2020). Effect of IBA concentration and cultivars on number of leaves, leaf area and chlorophyll content of leaf in Pomegranate (Punica granatum L.) cuttings under tempera conditions of Kashmir. Journal of Pharmacognosy and Phytochemistry, SP6, 86–90. http://www.phytojournal.com
Sabatino, L., D’Anna, F., & Iapichino, G. (2014). Cutting type and IBA treatment duration affect Teucrium fruticans adventitious root quality. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(2), 478–481. https://doi.org/10.1583/nbha4229611
Saha, R., Ginwal, H. S., Chandra, G., & Barthwal, S. (2021). A comparative study on grey relational analysis and C5.0 classification algorithm on adventitious rhizogenesis of Eucalyptus. Trees - Structure and Function, 35(1), 43–52. https://doi.org/10.1007/s00468-020-02008-4
Salehi, B., Mishra, A. P., Shukla, I., Sharifi-Rad, M., Contreras, M. del M., Segura-Carretero, A., Fathi, H., Nasrabadi, N. N., Kobarfard, F., & Sharifi-Rad, J. (2018). Thymol, thyme, and other plant sources: Health and potential uses. Phytotherapy Research, 32(9), 1688–1706. https://doi.org/10.1002/ptr.6109
Setiawan, E. (2017). Efektivitas pemberian IAA, IBA, NAA, dan root-up pada pembibitan kesemek. Jurnal Hortikultura Indonesia, 8(2), 97. https://doi.org/10.29244/jhi.8.2.97-103
Sevik, H., & Guney, K. (2013). Effects of IAA, IBA, NAA, and GA3 on rooting and morphological features of melissa officinalis L. stem cuttings. The Scientific World Journal, 2013(2001). https://doi.org/10.1155/2013/909507
Shalini Kaushik, & Neeraj Shukla. (2020). A review on effect of IBA and NAA and their combination on the rooting of stem cuttings of different ornamental crops. Journal of Pharmacognosy and Phytochemistry, 9(3), 1881–1885. http://www.phytojournal.com/archives/?year=2020&vol=9&issue=3∂=AE&ArticleId=11588
Suarmi, F., Sukerta, I., & Ananda, K. (2020). Penggunaan zat perangsang tumbuh indole butyric acid (IBA) pada stek kembang kertas ( Bougainvillea spectabilis ). Agrimeta, 10(19), 38–41.
Tustiyani, I. (2017). Pertumbuhan stek kopi the effect of various natural plant growth regulator on growth cuttings of coffee. Jurnal Pertanian, 8, 46–50. file:///F:/Jurnal Kopi/565-1984-2-PB.pdf
Wiraswati, S. F., & Badami, K. (2018). Pengaruh pemberian IBA dan asal stek terhadap pertumbuhan vegetatif kumis kucing. Agrovigor: Jurnal Agroekoteknologi, 11(2), 65–70. https://doi.org/10.21107/agrovigor.v11i2.4392
Wulandari Fitria, Murti Astiningrum, & Tujiyanta. (2017). Pengaruh jumlah daun dan macam media tanam pada pertumbuhan stek jeruk nipis (Citrus aurantifolia Swingle). Jurnal Ilmu Pertanian Tropika Dan Subtropika, 2(2), 48–51. https://translate.google.com/translate?hl=en&sl=id&u=http://jurnal.untidar.ac.id/index.php/vigor/article/view/487/394&prev=search
Yunus, A., Rahayu, M., Samanhudi, Pujiasmanto, B., & Riswanda, H. (2016). Respon kunir putih (Kaempferia rotunda) terhadap pemberian IBA dan BAP pada kultur in vitro. Agrosains : Jurnal Penelitian Agronomi, 18(June), 44–49.
Zaman, M. A. K., Azzeme, A. M., Ramle, I. K., Normanshah, N., Ramli, S. N., Shaharuddin, N. A., Ahmad, S., & Abdullah, S. N. A. (2020). Induction, multiplication, and evaluation of antioxidant activity of polyalthia bullata callus, a woody medicinal plant. Plants, 9(12), 1–21. https://doi.org/10.3390/plants9121772