Isolasi dan Karakterisasi Fitase dari Mikroba yang Terdapat pada Pupuk Kompos, Rumen Sapi, Ragi dan Tanah Sawah

Prof. Dr.rer.nat. Sajidan

Abstract

 

            This research was aimed to isolate and characterize  phytase from different sources of higger phosphat complex compounds (compost, cattle rumen, and yeast). Bacteria of phytase was isolated on Luria Bertani (LB) medium and it was incubated on 37 oC for 16 hours. Microbe from yeast was isolated on Pottato Dextrose Agar (PDA) medium. Crude enzyme from supernatant was extracted with centrifuge on 5.000 rpm for 5 minutes. Crude enzyme was characterized for optimum pH, temperature, and influence of matalo ion efector on relative activity of enzyme. B1 isolate from P88 compost had optimum relative activity on pH 5, temperature 50 oC and activator Zn2+ (10-3 and 10-4 M). B2 Isolate from cattle rumen had optimum relative activity on pH 5, temperature 50 oC and activator Zn2+ (10-3 M) and Mg2+ (10-4 M). B3 isolated from soy sauce yeast had optimum relative activity on pH 5, temperature 60 oC, and  activators Mg2+ (10-3 M) and, Fe2+ (10-4 M). B4 isolated from tempeh yeast had optimum relative activity on pH 5, temperature 50 oC and activators Mg2+ (10-3 M) and Ca2+ (10-4 M).

 

Key words: phytase, optimum pH, optimum temperature, metalo ion efector, isolate.


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References

Applegate, T. J. dan R. Angel 2004. Phytase: Basics of Enzme Function. E-book: Farm Animal Management at Purdue. Departement of Animal Science. Purdue University.

Bergman, E. L., K. Autio dan A. S. Sandberg, 2000. Optimal conditions for phytate degradation, estimation of phytase activity and localization of phytate in barley (Cv. blenheim). J. Agric. Food Chem. 48:4647-4655.

Dionysius, D. A., K. S. Hoek, J. M. Milne dan S. L. Slattery, 1993. Trypsin like enzyme from Sand Crab (Portunus pelagicus): Purification and characterization. J.Food Sci. 58:780 784.

Golovan, S., G. Wang, J. Zhang dan C. W. Forsberg, 2000. Characterization and overproduction of the E. coli appA encoded bifunctional enzyme that exhibits both phytase and acid phosphatase activities. J. Can. Microbiol. 46:59-71.

Greiner, R., E. Haller, U. Konietzny, and K.D. Jany. 1993. Purification and characterization of two phytases from Escherichia coli. Arch. Biochem. Biophys. 303: 107-113.

Greiner, R., E. Haller, U. Konietzny, and K.D. Jany. 1997. Purification and characterization of a phytase from Klebsiella terrigena. Arch. Biochem. Biophys. 341: 201-206.

Ismadi, H. M., 1987. Metoda Analisis Enzimatis. PAU Bioteknologi, Universitas Gadjah Mada, Yogyakarta.

Linder, M., Fanni, M. Parmentier, M. Sergent dan R. P. T. Luu, 1995. Protein recorvery from veal bones by enzymatic hydrolysis. J. Food Sci. 60: 949 952.

Maenz, D. D., 2005. Enzymatic Characteristic of Phytases as They Relate to Their Use in Animal Feeds. In E-Book: Enzyme in Animal Nutrition. M. R. Bedford dan G. G. Partridge, Eds. CABI Pub., UK.

Nelson, D. L. dan M. M. Cox, 2000. Lehninger Principles of Biochemistry. 3rd ed. Worth Pub., New York.

Oh, B. C., B. S. Chang, K. H. Park, N. C. Ha, H. K. Kim, B. H. Oh, and T. K., Oh, 2001. Calcium-Dependent Catalytic Activity of a Novel Phytase from Bacillus amyloliquefaciens DS11. J. Biochemistry. 40: 9669-9676.

Pasamontes, L., M. Haiker, M. Wyss, M. Tessier dan Van Loon, 1997. Gene cloning, purification and characterization of a heat-stable phytase from fungus A. fumigatus. J. App. Envi. Microbiol. 63: 1696-1700.

Rahayu, K., 1991. Teknologi Enzim. Pusat Antar Universitas Pangan dan Gizi, Universitas Gadjah Mada, Yogyakarta.

Reed, G., 1975. Enzmes in Food Processing. Academic Press, New York.

Sajidan. 2002. Molekulare Characterisierung einer Phytase (Myo-inositol Hexakiphosphate Hydrolase) und von Phosphatasen aus Bakterieisolaten Indoneschicher Reisfelder (Klebsiella pneumoniae). Dissertation. Institut fuer Biologie. Humboldt Universität zu Berlin. Deutschland (Germany).

Sajidan, 2004. Aplikasi Enzim Fitase untuk Campuran Pakan Ternak Unggas. Dalam: Seminar Nasional Sosialisasi dan Promosi Hasil Penelitian. UNS, Surakarta.

Sajidan, A.M.P. Nuhriawangsa dan A. Ratriyanto, 2004. Aplikasi Bakteri Penghasil Fitase (non rekombinan) pada Pakan Campuran Wheat Pollard terhadap Performan Ayam Broiler. Buletin Peternakan UGM . Vol. 28(3): 114-121.

Sajidan, A. M. P. Nuhriawangsa dan A. Ratriyanto, 2005. Aplikasi Eschericia coli dan Klebsiella pneumoniae Penghasil Fitase dan Kombinasinya pada Pakan Campuran Wheat Pollard terhadap Performan Ayam Broiler. Dalam: Proseding Seminar Nasional Pengembangan Usaha Peternakan Berdaya Saing di Lahan Kering. UGM, Yogyakarta: 186-192.

Shin, S., N.C. Ha, B.C. Oh, T.K. Oh, and B.H. Oh. 2001. Enzyme mechanism and catalytic property of  propeller phytase. Structure. 9:851-858.

Suharsono, 1990. Enzimologi. PAU Pangan dan Gizi, Universitas Gadjah Mada, Yogyakarta.

Volfova, O., J. Dvorakova, A. Hanzlikova dan A. Jandera, 1994. Phytase from A. niger. J. Folia Microbiology. 39:481-484.

Volk W.A. dan M.F. Wheeler, 1984. Basic Mikrobiology. Edisi ke-5. Penterjemah: Markham. Penerbit Erlangga, Jakarta.

Whyss., M., R. Brugger, A. Kronenbegger, R. Remy, R. Fimbel, G. Oesterhelt dan M. Lehmann, 1999. Biochemical characterization of fungal phytase (myo-inositolhexakisphosphat phosphohydro-lase: Catalytic properties. J. App. Environ. Mirobiology. 65:367-373.

Yankee, L. J., L. B. Selinger, and K. J. Cheng, 1999. Phytase activity of Selenomonas ruminantium: a preliminary character-ization. J. App. Microbiology. 29: 20-25.

DOI: https://doi.org/10.20961/sainspet.v7i1.1018

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