Effect of Moringa Leaves Ethanolic Extract (Moringa oleifera, Lam.) on Testicles Histopathological Structure in Metabolic Syndrome Rat Model

Febriagi Bayu Aji, Novan Adi Setyawan, Fikar Arsyad Hakim, Riza Novierta Pesik

Abstract

Introduction: Metabolic syndrome was a collection of various disorders that can significantly increase the risk of experiencing insulin resistance, atherosclerotic cardiovascular disease, vascular complications, and diabetes mellitus. The detrimental effect of this condition has led to the development of various treatments options, such as Moringa leaves extract (Moringa oleifera), which was suspected to have ameliorative effects. Therefore, this study aimed to determine the effect of Moringa leaves extract on the histopathological appearance of the testes of Wistar rats induced with metabolic syndrome.

Methods: This was an experimental design study, with a post-test-only control group, involving 30 Wistar rats (Rattus norvegicus). Metabolic syndrome was induced in the samples through the administration of high-fat and fructose feeds along with streptozotocin and nicotinamide. Furthermore, the moringa leaves extract was administered to the treatment groups in different doses of 150, 250, and 350 mg/kg BW. Testicular histopathological damage scores were then assessed on each group. The Kruskal-Wallis test which followed by Mann-Whitney test was used to determine the significant differences between the groups, while the correlation was analyzed using Spearman test.

Results: There were significant differences in the histopathological scores between the treatment and control groups. Furthermore, the Wistar rats treated with Moringa extract showed better histopathological scores (2.1±0.64) compared to those without the treatment (2.7±0.54), p<0.05. The results also showed that higher doses of the extract significantly correlated with better outcome in testicular histopathological structure of metabolic syndrome rat model (p=0.000, r=-0.396).

Conclusion: Moringa leaves ethanolic extract treatment improved testicular histopathological appearance in Wistar rats induced with metabolic syndrome.

Keywords

metabolic syndrome; moringa leaves; testes; white rat

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References

1. Jun S, Ha K, Chung S, Joung H. Meat and milk intake in the rice-based Korean diet: Impact on cancer and metabolic syndrome. Proc Nutr Soc. 2016;75(3):374–84.

2. Suhaema, Masthalina H. Pola konsumsi dengan terjadinya sindrom metabolik di Indonesia. J Kesehat Masy Nas. 2015;9(4):340–7.

3. Aulia D, Ayu SF, Nefonafratilova N. Hubungan gaya hidup, riwayat penyakit, dan ekonomi, dengan kejadian strok pada suku mandailing di RSUD Kota Padangsidimpuan tahun 2017. Sel J Penelit Kesehat. 2018;5(2):50–8.

4. Saklayen MG. The global epidemic of the metabolic syndrome. Curr Hypertens Rep. 2018;20(2):1–8.

5. Nowotny K, Jung T, Höhn A, Weber D, Grune T. Advanced glycation end products and oxidative stress in type 2 diabetes mellitus. 2015;194–222.

6. Alahmar AT. Role of oxidative stress in male infertility: An updated review. 2019;4–18.

7. Balamuthu K. Evaluation of anti-fertility activity of ethanolic extract of Cassia fistula ( linnaeus ) leaf on male albino rats. Am J Pharmtech Res. 2012;1(5):613–23.

8. Fatoba TA, Faleyimu OI, Adebayo AJ. Effects of increasing aqueous root extract of Moringa oleifera on sperm production of albino rats. Agrosearch. 2013;13(1):29–36.

9. Vergara-jimenez M, Almatrafi MM, Fernandez ML. Bioactive components in Moringa oleifera leaves protect against chronic disease. Antioxidants. 2017;6(91):1–13.

10. Moichela FT, Adefolaju GA, Opuwari CS, Henkel RR. Aqueous leaf extract of Moringa oleifera reduced intracellular ros production , dna fragmentation and acrosome reaction in human spermatozoa in vitro. 2020;1–11.

11. Jannah R, Luh N, Setiasih E, Suastika P, Veteriner LH, Hewan FK, et al. Histopatologi testis tikus penderita diabetes mellitus pasca pemberian ekstrak daun kelor. 2018;10(2):176–82.

12. Nur T, Putri W, Budiani DR. Pengaruh ekstrak etanolik daun kelor ( Moringa oleifera , lam ) terhadap nilai ekspresi TNF- α testis tikus wistar ( rattus norvegicus ) model sindrom metabolik. 2022;1(4):130–8.

13. Priya PH, Reddy P. Effect of restraint stress on lead-induced male reproductive toxicity in rats. J Exp Zool Part A Ecol Genet Physiol. 2012;317(7):455–65.

14. Suman KR, Mohanty IR, Borde MK, Maheshwari U, Deshmukh YA. Development of an experimental model of nephrotoxicity co-existing with obesity in rats. Indian J Pharm Sci. 2018;80(5):844–51.

15. Nolan PB, Carrick-Ranson G, Stinear JW, Reading SA, Dalleck LC. Prevalence of metabolic syndrome and metabolic syndrome components in young adults: A pooled analysis. Prev Med Reports [Internet]. 2017;7:211–5. Available from: http://dx.doi.org/10.1016/j.pmedr.2017.07.004

16. Martins AD, Majzoub A, Agawal A. Metabolic syndrome and male fertility. World J Mens Health. 2019;37(2):113–27.

17. Soiza RL, Donaldson AIC, Myint PK. Metabolic syndrome: Pathophysiology, management, and modulation by natural compounds. Ther Adv Vaccines. 2017;11(8):215–25.

18. Welty FK, Alfaddagh A, Elajami TK. Targeting inflammation in metabolic syndrome. 2019;176(5):139–48.

19. Ogunlade B, Jeje SO, Adelakun SA, Akingbade GT. Moringa oleifera restored semen quality, hormonal profile, and testicular morphology against highly active antiretroviral therapy-induced toxicity in adult male wistar rats. J Bras Reprod Assist. 2022;26(1):3–12.

20. Sugiantari IAP, Suaskara IBM, Suarni NMR. Konsentrasi spermatozoa dan ketebalan tubulus seminiferus tikus putih jantan setelah pemberian seduhan daun kelor (Moringa oleifera l.). Metamorf J Biol Sci. 2020;7(2):97.

21. Prabsattroo T, Wattanathorn J, Iamsaard S, Somsapt P, Sritragool O, Thukhummee W, et al. Moringa oleifera extract enhances sexual performance in stressed rats. J Zhejiang Univ Sci B. 2015;16(3):179–90.

22. Carresi C, Gliozzi M, Musolino V, Scicchitano M, Scarano F, Bosco F, et al. The effect of natural antioxidants in the development of metabolic syndrome: focus on bergamot polyphenolic fraction. Nutrients. 2020;12(5):1–24.

23. Dhakad AK, Ikram M, Sharma S, Khan S, Pandey V V., Singh A. Biological, nutritional, and therapeutic significance of Moringa oleifera Lam. Phyther Res. 2019;33(11):2870–903.

24. Mbikay M. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: A review. Front Pharmacol. 2012;3(24):1–12.

25. Elekofehinti OO. Saponins: Anti-diabetic principles from medicinal plants - A review. Pathophysiology [Internet]. 2015;22(2):95–103. Available from: http://dx.doi.org/10.1016/j.pathophys.2015.02.001

26. Moghimipour E, Handali S. Saponin: Properties, methods of evaluation and applications. Annu Res Rev Biol. 2015;5(3):207–20.

27. Fidrianny I, Kanapa I, Singgih M. Phytochemistry and pharmacology of Moringa tree: An overview. Biointerface Res Appl Chem. 2021;11(3):10776–89.

28. Kolodziej H, Burmeister A, Trun W, Radtke OA, Kiderlen AF, Ito H, et al. Tannins and related compounds induce nitric oxide synthase and cytokines gene expressions in Leishmania major-infected macrophage-like RAW 264.7 cells. Bioorganic Med Chem. 2005;13(23):6470–6.

29. Udeh NE, Anaga A, Asuzu IU. Effects of Gnetum africanum (Welw) methanol leaf extract on weight and haematological profile of wistar rats following chronic oral administration. Sokoto J Vet Sci. 2018;16(3):49.

30. Alkafafy ME, Sayed SM, El-Shehawi AM, El-Shazly S, Farouk S, Alotaibi SS, et al. Moringa oleifera ethanolic extract ameliorates the testicular dysfunction resulted from HFD-induced obesity rat model. Andrologia. 2021;53(8):1–12.

DOI: https://doi.org/10.13057/smj.v6i1.69243

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