Mesenchymal Stem Cell-Derived Secretome Modulates Apoptotic And Inflammatory Markers In The Hippocampus Of Septic Mice

Rahmat Nugroho, Arifin Arifin, Ratih Arianita Agung

Abstract

Introduction: Sepsis is a global health problem that significantly increases morbidity and mortality worldwide. The inflammatory process in sepsis can cause damage to the hippocampus, leading to brain dysfunction mediated by apoptosis (Caspase-3) and neuroinflammation (TNF-α). Mesenchymal stem cells (MSCs) have potential as a regenerative therapy to reduce brain damage. This study aims to assess the therapeutic effect of MSC secretome on hippocampus damage, measured through Caspase-3 and TNF-α expression, in a mice model of sepsis.

Methods: This experimental study used a post-test-only randomized controlled trial (RCT) design. Male mice (Mus musculus Balb/C) were divided into five groups: normal control (NC), sepsis control (SC) induced with LPS, and three treatment groups induced with LPS and given MSC secretome at doses of 150 μL, 300 μL, and 600 μL. Caspase-3 and TNF-α expression levels in the hippocampus were measured using immunohistochemistry (IHC). Statistical analysis was performed using ANOVA and Kruskal-Wallis tests (p < 0.05).

Results: Administration of MSC secretome did not result in a statistically significant reduction in Caspase-3 (p=0.730) or TNF-α (p=0.135) levels in the hippocampus. Although average values were lower in the treatment groups compared to the sepsis control group, the differences were not statistically significant. The greatest reduction in Caspase-3 occurred in the group receiving 600 μL secretome , while the lowest average TNF-α was observed in the 600 μL dose group.

Conclusion: Mesenchymal stem cell secretome administration reduced Caspase-3 and TNF-α levels in the hippocampus of a mice sepsis model, but the impact was not statistically significant.

Keywords

Secretome, mesenchymal stem cells, Caspase-3, TNF-α, hippocampus, sepsis

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References

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DOI: https://doi.org/10.20961/tscm.v3i1.95057

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