THE EFFECT OF MESENCHYMAL SECRETOME STEM CELL THERAPY TOWARD CASPASE-3 AND TNF-α EXPRESION IN HIPPOCAMPUS MICE MODEL OF SEPSIS
Abstract
Introduction: Sepsis is a global health problem that significantly increases morbidity and mortality worldwide. The inflammatory process in sepsis can cause damage, including to the hippocampus in the brain, potentially leading to brain dysfunction. Apoptosis mediated by caspase-3 and neuroinflammation exacerbated by TNF-α are the main mechanisms involved in this damage. Mesenchymal stem cells (MSCs) have the potential as a promising regenerative therapy to reduce brain damage by inhibiting apoptosis and reducing neuroinflammation. This study aims to assess the therapeutic effect of MSC secretome on hippocampus damage, measured through caspase-3 and TNF-α expression, in mice model of sepsis.
Methods: This experimental study used a post-test-only randomized controlled trial (RCT) design with a control group. Male mice of the Mus musculus Balb/C strain were divided into five groups: normal control (NC), sepsis control (SC) induced with LPS, and three treatment groups induced with LPS and given MSC secretome at doses of 150 μL, 300 μL, and 600 μL. Caspase-3 and TNF-α expression levels in the hippocampus were measured using immunohistochemistry. Statistical analysis was performed using ANOVA and Kruskal-Wallis tests, with significance considered at p < 0.05.
Results: Administration of mesenchymal stem cell secretome in the mice sepsis model did not result in a significant reduction in Caspase-3 or TNF-α levels in the hippocampus. Although Caspase-3 and TNF-α levels were lower in the treatment groups compared to the control group, the differences were not statistically significant. The greatest reduction in Caspase-3 occurred in the group receiving LPS and 600 μL secretome, but it still did not show a significant difference from the other groups (p=0.730 for Caspase-3 and p=0.135 for TNF-α).
Conclusion: Mesenchymal stem cell secretome administration reduced Caspase-3 and TNF-α levels in the hippocampus mice model of sepsis, but does not have a statistically significant impact.
Keywords
References
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