Phenotype of Conditioned Medium (Secretome) Umbilical Cord Mesenchymal Stem Cells and Effects On Nf-Κb Levels in The Pathogenesis of Liver Fibrosis Hepg2 Cell in Vitro

Abstract

Liver fibrosis resulting from an imbalance in the synthesis and degradation of the extracellular matrix (ECM). Hepatoma G2 (HepG2) cells are the most widely used in various in vitro studies. Nuclear factor kappa B (NF-κB) overaction in hepatocytes promotes inflammation in the liver by increasing pro-inflammatory production. The stem cell secretomes contribute to hepatic injury mechanisms by engaging in regenerative mechanisms with actions on hepatocytes, stellate cells, and macrophages. This study aims to determine the phenotypic characteristics and analyze the effect of secretomes on NF-κB levels in the pathogenesis of liver fibrosis HepG2 cells in vitro. Research was conducted at the Prodia Stem Cell Laboratory, Jakarta using the secretome of mesenchymal stem cells of the newborn's umbilical cord and then cultured. AssayNF-κB was performed on HepG2 cells administered with and without a secretome. Isolation of the secretome obtained CD 73+, CD90+, CD105+, and Lin- phenotypes and their parameters included BDNF, SDF-1, FGF, VEGF, PDGF, EGF, NGF, IL- 10, IL-1β, IFN-γ, MCP-1, IL-6, IL-12p70, IL-17A, IL-18, and IL-23. There was a significant difference in 24-hour (p=<0.001) and 48-hour (p=<0.001) levels of NF-kβ between mesenchymal stem cell secretome group and the control group.This study showed a conditioned medium (secretome) phenotype, namely CD 73+, CD90+, and CD105+ and there was a significant effect between cord mesenchymal stem cell secretomes and decreased levels of NF-κB in the pathogenesis of liver fibrosis HepG2 cells in vitro.