IDENTIFIKASI KERAGAMAN GENETIK KERBAU LOKAL POPULASI JAWA TIMUR DAN NUSA TENGGARA BARAT BERBASIS MIKROSATELIT SEBAGAI MODEL PENGEMBANGAN KONSERVASI KERBAU SECARA EX SITU

Abstract

In Indonesia, local buffalo (Bubalus bubalis) is one type of germplasm that a major contribution in the national meat supply of animal protein. However, this germplasm will be reduced of its number.  If this condition continues we will loss the germplasm . One effort to this is conservatuin based on  a molecular marker mikrosatellite . Selected marker locus are HEL09 , INRA23 and ISLTS05 . Sampling area of it research in East Java and West Nusa Tenggara . The results showed that in both are and all loci is polymorphic, allele frequencies ranging from 0.125 to 0.875 and heterozygosity values in the range 35-65 % . These results illustrate that the two populations have genetic diversity that is still quite varied genotype .

Key Words: alele frequency, heterozigosity, local buffalo, microsatellite