Identifikasi dan Penentuan Indeks Hidrolisis Protein pada Bakteri Proteolitik dari Tanah Mangrove di Margomulyo, Balikpapan
Abstract
of aquatic fauna. The decaying mangrove stems contain vegetable protein and mixed with animal protein
derived from dead animals in mangrove soil. Proteolytic bacteria play a role in the process of protein
degradation into more simple and soluble compounds. This research aims to: 1) identify species of proteolytic
bacteria in mangrove soil, 2) count the Protein Hydrolysis Index on each species of proteolytic bacteria, 3)
determine the most potential proteolytic bacteria species to decompose the protein. Soil samples were taken
from 10 points of mangrove area Margomulyo, Balikpapan. After that, soil samples were diluted in 10-1 to 10-
10 and 0,1 mL of each level dilution is inoculated to the NA medium, incubated at 37°C for 1 x 24 hours. Each
colony sorts were isolated, identified and determined the proteolytic bacteria isolates, then the Protein
Hydrolysis Index on the Skim Milk Agar medium were count. The research results showed that: 1) there are 7
species of proteolytic bacteria from mangrove soil, 2) the Protein Hydrolysis Index of each species of
proteolytic bacteria ranged from 1.62 to 2.94, and 3) Vibrio parahaemolyticus is the most potential proteolytic
bacteria with the Protein Hydrolysis Index is 2,94
Keywords
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