Pengaruh Teknik Sterilisasi dan Komposisi Medium terhadap Pertumbuhan Tunas Eksplan Sirsak Ratu

Rico Hutama Sulistiyo, Zayyan Luthfiyyah, Buana Susilo, Lengga Nurullah Dalimartha, Eko Chandra Wiguna, Nuniek Yuliana, Nugroho Prasetyo

Abstract

Successful tissue culture of soursop plants requires proper sterilization techniques and hormone levels to
produce good shoot growth. This study aims to determine the effect of sterilization techniques and medium
composition of soursop explants, using factorial completely randomized design with 2 factors: sterilizing agents
and medium composition, repeated 3 times. Hydrogen Peroxide, Mercury and Sodium Hypoclorite used as
sterilizing agents. The medium composition used in this study consisted of 3 levels: MS 0, MS 0.5 ppm BAP
+ 0.05 ppm NAA and MS 1 ppm BAP + 0.1 ppm NAA. The best treatment is MS medium 0.5 ppm BAP + 0.05
ppm NAA with hydrogen peroxide sterilizing agent which produces the highest propagation level and lowest
contamination level.

Keywords

Ratu soursop, sterilization, BAP, NAA

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